A5016107770- Plant Reproductive Biology
- Plant Molecular Biology Research
- Polysaccharides and Plant Cell Walls
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Toxin Mechanisms and Immunotoxins
- Plant tissue culture and regeneration
- Botany and Plant Ecology Studies
- Advanced Fluorescence Microscopy Techniques
- Monoclonal and Polyclonal Antibodies Research
- Plant Stress Responses and Tolerance
- Microtubule and mitosis dynamics
- Polysaccharides Composition and Applications
- Tree Root and Stability Studies
- Transgenic Plants and Applications
- Cellular Mechanics and Interactions
Czech Academy of Sciences, Institute of Experimental Botany
2014-2025
Charles University
2014-2021
Czech Academy of Sciences
2008
Plant cell morphogenesis involves concerted rearrangements of microtubules and actin microfilaments. We previously reported that FH1, the main Arabidopsis thaliana housekeeping Class I membrane-anchored formin, contributes to dynamics microtubule stability in rhizodermis cells. Here we examine effects mutations affecting FH1 (At3g25500) on above-ground organ development seedlings, as well cytoskeletal organization dynamics, using a combination confocal variable angle epifluorescence...
Plant cells respond to a wide range of stimuli through intracellular calcium (Ca2+) signaling. Cyclic nucleotide-gated channels (CNGCs) are major class plant Ca2+ channels, with 20 homologs in Arabidopsis. These tetrameric plasma membrane proteins act downstream diverse signals, such as phytohormones, extracellular damage, cell wall integrity or temperature. Here, we identify plant-specific proteins, Armadillo Repeat Only (ARO), essential regulators possibly all CNGCs. Abrogation functional...
The ARP2/3 complex and formins are the only known plant actin nucleators. Besides their actin-related functions, both systems also modulate microtubule organization dynamics. Loss of main housekeeping Arabidopsis thaliana Class I membrane-targeted formin FH1 (At3g25500) is to increase cotyledon pavement cell lobing, while mutations affecting subunits exhibit an opposite effect. Here we examine role subunit ARPC5 (At4g01710) in epidermal morphogenesis with focus on cells trichomes using a...
Cytoskeleton can be observed in live plant cells situ with high spatial and temporal resolution using a combination of specific fluorescent protein tag expression advanced microscopy methods such as spinning disc confocal (SDCM) or variable angle epifluorescence (VAEM). Existing for quantifying cytoskeletal dynamics are often either based on laborious manual structure tracking, depend costly commercial software. Current automated also do not readily allow separate measurements lifetime,...
Development of the plant aerial organs epidermis involves a complex interplay cytoskeletal rearrangements, membrane trafficking-dependent cell surface expansion, and intra- intercellular signaling, resulting in pattern perfectly interlocking pavement cells. While recent detailed vivo observations convincingly identify microtubules rather than actin as key players at early stages development lobes Arabidopsis, mutations affecting actin-nucleating ARP2/3 are long known to reduce lobing,...
Abstract Keyhole limpet hemocyanin (KLH)‐conjugated peptides are routinely used to raise polyclonal antibodies for biochemical or immunolocalization studies. Rats suitable producing antisera against plant antigens as they often lack non‐specific response towards materials. We attempted obtain rat derived from several proteins. However, most recognized the same background KLH‐related antigen (KRAP) in Arabidopsis and tobacco. characterized KRAP with respect size cellular localization examined...
Abstract Formins are evolutionarily conserved eukaryotic proteins engaged in actin nucleation and other aspects of cytoskeletal organization. Angiosperms have two formin clades with multiple paralogs; typical plant Class I formins integral membrane that can anchor structures to membranes. For the main Arabidopsis housekeeping formin, FH1 (At3g25500), plasmalemma localization was documented heterologous expression overexpression studies. We previously showed loss function increases cotyledon...