A5051949154- Luminescence and Fluorescent Materials
- Nanoplatforms for cancer theranostics
- Polymer Surface Interaction Studies
- Supramolecular Chemistry and Complexes
- Crystallization and Solubility Studies
- Supramolecular Self-Assembly in Materials
- X-ray Diffraction in Crystallography
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Molecular Sensors and Ion Detection
- Advanced Polymer Synthesis and Characterization
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Click Chemistry and Applications
- Epigenetics and DNA Methylation
- 3D Printing in Biomedical Research
- Advanced biosensing and bioanalysis techniques
- Pluripotent Stem Cells Research
- Immune Cell Function and Interaction
- Phonetics and Phonology Research
- CAR-T cell therapy research
- Surface Modification and Superhydrophobicity
- Electrospun Nanofibers in Biomedical Applications
- Nanoparticle-Based Drug Delivery
University of Rochester Medical Center
2025
Huazhong Agricultural University
2022-2024
Tongji University
2014-2024
Zhongnan Hospital of Wuhan University
2024
Wuhan University
2024
University at Buffalo, State University of New York
2024
East China Normal University
2023
University of Hong Kong
2017-2022
Hong Kong University of Science and Technology
2017-2022
Wuhan Union Hospital
2021
Acrylonitriles with aggregation-induced emission (AIE) characteristics have been found to show promising applications in two-photon biomedical imaging. Generally, elaborate synthetic efforts are required achieve different acrylonitriles distinct functionalities. In this work, we first reported the synthesis of two group-functionalized AIE-active (TPAT-AN-XF and 2TPAT-AN) obtained simply by mixing same reactants at temperatures using a facile transition metal-free method. These AIE luminogens...
Abstract Bacterial infections pose a serious threat to human health. Photodynamic therapy is an effective medical treatment solve the problems raised by antibiotic resistant bacteria. But it not easy have photosensitizers (PSs) that can simultaneously produce efficient fluorescence and reactive oxygen species. Traditional PSs show compromised performances due aggregation‐caused quenching effect in aqueous media, however, luminogens with aggregation‐induced emission (AIE) inherently achieve...
The efficient bioconjugation of functional groups/molecules to targeted matrix and bio-related species drives the great development material science biomedicine, while dilemma metal catalysis, uneasy premodification, limited reaction efficiency in traditional has restricted booming some extent. Here, we provide a strategy for metal-free click at diverse levels based on activated alkynes. As proof-of-concept, abundant native groups including amine, thiol, hydroxyl can directly react with...
An AIE-active ratiometric probe for the first time achieved long-term quantification of lysosomal pH during medaka larva's caudal fin regeneration.
Abstract Background Although chimeric antigen receptor T (CAR-T) cells have been proven to be an effective way of treating B cell malignancies, a lot patients could not benefit from it because failure in CAR-T manufacturing, disease progression, and unaffordable price. The study aimed explore universal products extend the clinical accessibility. Methods antitumor activity CRISPR/Cas9-edited allogeneic anti-CD19 (CAR-T19) was assessed vitro, animal models, with relapsed/refractory (R/R) acute...
We describe here a simple fluorometric assay for the highly sensitive detection of caspase-3 activities on basis inner-filter effect gold nanoparticles (AuNPs) CdTe quantum dots (QDs). The method takes advantage high molar absorptivity plasmon band as well large absorption shift from 520 to 680 nm upon nanoparticle aggregation. When labeled with peptide possessing cleavage sequence (DEVD), monodispersed Au-Ps (peptide-modified AuNPs) exhibited tendency aggregate when exposed caspase-3, which...
An antibody-AIEgen conjugate is designed and developed as a "turn-on" fluorescent probe for wash-free specific cancer cell imaging. The cetuximab-conjugated AIEgen shows red fluorescence only when it internalized accumulated in cells with overexpressed epidermal growth factor receptor through endocytosis. first lights up the lysosomes. After hydrolysis, its residue mitochondria, making them highly emissive long retention time. Compared conventional "always-on" probes, exhibits very good...
Fast surface immobilization of native bioconjugates through a spontaneous amino-yne click reaction is realized.
Abstract Immunogenic cell death (ICD) plays a crucial role in triggering the antitumor immune response tumor microenvironment (TME). Recently, considerable attention has been dedicated to ferroptosis, type of ICD that is induced by intracellular iron and demonstrated change desert status TME. However, among cancers are characterized an desert, such as prostate cancer, strategies for inducing high levels ferroptosis remain limited. Radiated cell-derived microparticles (RMPs) radiotherapy...
Abstract Vaccine‐based therapeutics for cancers face several challenges including lack of immunogenicity and tumor escape pathways single antigen targets. It has been reported that radiotherapy an in situ vaccine effect provides antigens following irradiation, helping to activate antigen‐presenting cells (APCs). Herein, a new approach is developed by combining genetically engineered irradiated cell debris (RTD) hyaluronic acid (HA), termed HA@RTD. A cancer line overexpresses...
Developing surfaces with antithrombotic properties is of great interest for the applications blood‐contacting biomaterials and medical devices. It promising to coimmobilize two or more biomolecules different complementary functions improve blood compatibility. However, general one‐pot strategy usually adopted by previous studies suffers problems inevitable competition between diverse uncontrollability relative quantities immobilized biomolecules. To solve these problems, a new sequential...
Efficient control of the self-renewal and pluripotency maintenance embryonic stem cell (ESC) is a prerequisite for translating technologies to clinical applications. Surface topography one most important factors that regulates behaviors. In present study, micro/nano topographical structures composed gold nanoparticle layer (GNPL) with nano-, sub-micro-, microscale surface roughnesses were used study roles these in regulating behaviors mouse ESCs (mESCs) under feeder-free conditions. The...
Embryonic stem cells (ESCs) can be induced to differentiate into nerve cells, endowing them with potential applications in the treatment of neurological diseases and neural repair. In this work, we report for first time that sulfated chitosan promote differentiation ESCs. As a type glycosaminoglycan analog, well-defined sulfation sites controlled degree (DS) were prepared through simple procedures influence on ESCs was investigated. Compared other sites, 6-O-sulfated showed most optimal...
A lipid-droplet-specific AIEgen was used to evaluate the inhibitory efficacy of HIF-1-targeting drugs by assessing lipid-droplet levels.
Abstract Brain metastases (BRM) are common in advanced lung cancer. However, their treatment is challenging due to the blood‐brain barrier (BBB) and immunosuppressive tumor microenvironment (ITME). Microparticles (MPs), a type of extracellular vesicle, can serve as biocompatible drug delivery vehicles that be further modulated with genetic engineering techniques. MPs prepared from cells induced different insults compared it found radiation‐treated cell‐released microparticles (RMPs) achieve...
Two Gram-negative, rod-shaped, motile, aerobic bacterial strains designated HY-14(T) and HY-24 were isolated from the sludge of an anaerobic ammonium-oxidizing bioreactor. The could not grow with 5 % (w/v) NaCl, did produce acid d-glucose or utilize d-glucose, gluconate citrate as a sole carbon source. Summed feature 3 C(16 : 0) most abundant fatty acids; hydroxyl acids C(12 3-OH C(10 also present. Fatty 2-OH was absent. DNA G+C contents 50.6+/-0.5 mol%. Phylogenetic analyses based on 16S...
It is well known that adsorbed proteins play a major role in cell adhesion. However, it has also been reported cells can adhere to protein-resistant surface. In this work, the behavior of L02 and BEL-7402 on protein-resistant, 3D topographical surface was investigated. The gold nanoparticle layer (GNPL) surfaces were prepared by chemical plating, topography described roughness parameters acquired from multiscale analysis. Both smooth Au GNPL modified with POEGMA polymer brushes using...
Well-controlled polymerization of N-vinylpyrrolidone (NVP) on Au surfaces by surface-initiated atom transfer radical (SI-ATRP) was carried out at room temperature a silanization method. Initial attempts to graft poly(N-vinylpyrrolidone) (PVP) layers from initiators attached alkanethiol monolayers yielded PVP films with thicknesses less than 5 nm. The combined factors the difficulty in controllable NVP and instability led controlled surfaces. Therefore, method employed form an adhesion layer...
Synthetic polymerization and supramolecular with sequence control are far from an easy task. Herein, a narcissistic self-sorting polymer is prepared of (−AA–BB−)n by using cucurbit[8]uril (CB[8])-based ternary complexes as monomers, which spontaneously formed heteroditopic AB-type guest CB[8]. Supramolecular the structural changes at each stage have been successfully demonstrated NMR, UV–vis, fluorescence spectra. The starts second step after formation different supra-monomers. dynamic...