A5101931380- Autophagy in Disease and Therapy
- Chronic Kidney Disease and Diabetes
- Metabolism, Diabetes, and Cancer
- Renal Diseases and Glomerulopathies
- Cancer-related molecular mechanisms research
- Calcium signaling and nucleotide metabolism
- TGF-β signaling in diseases
- Sirtuins and Resveratrol in Medicine
- Inflammatory Biomarkers in Disease Prognosis
- Diabetes Treatment and Management
- Berberine and alkaloids research
- Advanced Glycation End Products research
- RNA modifications and cancer
- Mesenchymal stem cell research
University of Science and Technology of China
2018-2022
Shandong University
2019
Anhui Provincial Hospital
2019
Background Previous studies have shown that a variety of biomarkers are closely related to the occurrence and development early-stage diabetic nephropathy (DN) in patients. The aim this study was evaluate role multiple sera urinary diagnosis DN patients with type 2 diabetes. Methods We enrolled 287 diabetes, who were classified into normoalbuminuria ( n = 144), microalbuminuria 94), or macroalbuminuria 49) groups based on their urine albumin creatinine ratios (UACR), along 42 healthy...
Background Berberine (BBR) has therapeutic effect on diabetic nephropathy (DN), but its molecular mechanism is not completely clear. Methods The DN model was established to observe the of BBR. expression levels lncRNA Gas5 were detected by PCR. transcriptional regulation CCAAT enhancer binding protein beta (C/EBPβ) analyzed chromatin immunoprecipitation quantitative PCR (ChIP-qPCR) and luciferase reporter gene assay. targeted between miR-18a-5p C/EBPβ 3′-untranslated region (3′-UTR) also...
Abstract Objectives Metformin (MET) has protective effect on diabetic nephropathy (DN). This study aims to demystify the mechanism of MET function in DN. Methods Mouse glomerular membrane epithelial cell line SV40-MES-13 was treated with normal or high glucose combined without MET. The relationships among H19, miR-143-3p and TGF-β1 were evaluated by luciferase reporter assay. MTT assay performed detect proliferation. levels inflammatory factors investigated enzyme-linked immunosorbent...
Hepatic gluconeogenesis is the major contributor to hyperglycemia in diabetes. Long noncoding RNA (lncRNA) maternally expressed gene 3 (MEG3) has been shown promote hepatic insulin resistance; however, underlying mechanism involving remains unclear. This study aims investigate potential role of MEG3 gluconeogenesis. Mouse primary hepatocytes were used this study. Cell transfection was performed for overexpression or knockdown specific genes. Expressions MEG3, miR-302a-3p, CREB-regulated...
Abstract The aim of this study was to investigate the role metformin in high glucose‐induced mesangial cell proliferation, inflammation and extracellular matrix (ECM) accumulation elucidate underlying mechanism function. An MTT assay used examine rat (RMC) proliferation. levels TNF‐α, IL‐6 TGF‐β RMCs were determined by ELISA. protein expression fibronectin, collagen IV autophagy‐related proteins (Beclin‐1, LC3‐I LC3‐II) detected using western blot. Fluorescence microscopy analysis carried...