A5080999953- 3D Printing in Biomedical Research
- Receptor Mechanisms and Signaling
- Microfluidic and Bio-sensing Technologies
- Gene Regulatory Network Analysis
- Innovative Microfluidic and Catalytic Techniques Innovation
- Single-cell and spatial transcriptomics
- Fibroblast Growth Factor Research
- Inhalation and Respiratory Drug Delivery
- Microfluidic and Capillary Electrophoresis Applications
- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- Advanced Fluorescence Microscopy Techniques
- Electrowetting and Microfluidic Technologies
- Sepsis Diagnosis and Treatment
- Synthesis and properties of polymers
- Neuroscience and Neural Engineering
- Blood transfusion and management
- Digital Imaging for Blood Diseases
- Nanopore and Nanochannel Transport Studies
- Nanofabrication and Lithography Techniques
- Cellular transport and secretion
- Protease and Inhibitor Mechanisms
- Pluripotent Stem Cells Research
- Angiogenesis and VEGF in Cancer
- Platelet Disorders and Treatments
Massachusetts Institute of Technology
2017-2023
Seoul National University
2014-2019
Vassar College
2019
UPMC Montefiore
2017
University of Pittsburgh
2017
University of Seoul
2017
Article30 November 2015Open Access Source Data Frequency modulation of ERK activation dynamics rewires cell fate Hyunryul Ryu School Mechanical and Aerospace Engineering, Seoul National University, Seoul, Korea Institute Advanced Machinery Design, Search for more papers by this author Minhwan Chung Maciej Dobrzyński System Biology Ireland, University College Dublin, Belfield, Ireland Dirk Fey Yannick Blum Department Biomedicine, Basel, Switzerland Sung Sik Lee Biochemistry, Zurich, Matthias...
Abstract The three canonical Rho GTPases RhoA, Rac1 and Cdc42 co-ordinate cytoskeletal dynamics. Recent studies indicate that all are activated at the leading edge of motile fibroblasts, where their activity fluctuates subminute time micrometer length scales. Here, we use a microfluidic chip to acutely manipulate fibroblast dynamics by applying pulses platelet-derived growth factor (PDGF) or kinase inhibitor Y-27632 (which lowers contractility). This induces acute robust membrane protrusion...
We used 3D cell printing to emulate an airway coupled with a naturally-derived blood vessel network in vitro. Decellularized extracellular matrix bioink derived from porcine tracheal mucosa (tmdECM) was encapsulate and print endothelial cells fibroblasts within designated polycarprolactone (PCL) frame. Providing niche that emulates conditions vivo, tmdECM gradually drives re-orientation, which leads the formation of lumen network. A fully-differentiated vitro model assembled printed vascular...
Understanding the mechanism behind cancer metastasis is a major challenge in biology. Several vitro models have been developed to mimic microenvironment by engineering cancer-endothelial cell (EC) and cancer-stromal interactions. It has challenging realistically angiogenesis, intravasation, extravasation using macro-scale approaches but recent progress microfluidics technology begun yield promising results. We present chip that produce microvessels, where EC stromal cells can be patterned...
We introduce a novel microfluidic device to co-culture blood vessel network and cell tissues in an <italic>in vivo</italic>-like niche.
A fully-automated and field-deployable blood leukocyte separation platform based on a new type of inertial microfluidic device, multi-dimensional double spiral (MDDS) which achieves sample focusing in single device without sheath flow.
Assessment of airway secretion cells, both for research and clinical purposes, is a highly desired goal in patients with acute chronic pulmonary diseases. However, lack proper cell isolation enrichment techniques hinder downstream evaluation characterization cells found secretions. Here, we demonstrate novel method to capture immune-related from secretions using closed-loop separation spiral inertial microfluidics (C-sep). By recirculating the output focusing stream back input reservoir...
In blood samples from patients with viral infection, it is often important to separate particles human cells, for example, minimize background in performing whole genome sequencing. Here, we present a microfluidic device that uses spiral inertial microfluidics continuous circulation host cells and free nucleic acid. We demonstrate this effectively reduces white red platelets both plasma excellent recovery of Furthermore, separation leads greater coverage depth, highlighting an application...
Acute lung injury is characterized by excessive extracellular matrix proteolysis and neutrophilic inflammation. A major risk factor for bacterial pneumonia. However, host factors that protect against pathogen-induced host-sustained proteolytic following infection are poorly understood. Pseudomonas aeruginosa (PA) a cause of nosocomial pneumonia secretes proteases to amplify tissue injury. We show thrombospondin-1 (TSP-1), matricellular glycoprotein released during inflammation,...
Article19 November 2019Open Access Source DataTransparent process Temporal perturbation of ERK dynamics reveals network architecture FGF2/MAPK signaling Yannick Blum Institute Cell Biology, University Bern, Switzerland Search for more papers by this author Jan Mikelson Department Biosystems Science and Engineering, ETH Zurich, Basel, Maciej Dobrzyński orcid.org/0000-0002-0208-7758 Hyunryul Ryu Advanced Machinery Design, Seoul National University, Seoul, Korea Marc-Antoine Jacques Noo Li Jeon...
There are many proposed mechanisms by which single cells can be trapped; among them is the through-hole membrane for characterization of individual microorganisms. Due to small scale fabricated pores, construction membranes on a large and with relatively areas faces difficulties. This paper describes novel fabrication methods large-area, freestanding micro/nano constructed from versatile materials using microfluidic chip (THMMC). process rapidly (<20 min) fabricate high fidelity multiscale...
The blood circulatory system links all organs from one to another support and maintain each organ's functions consistently. Therefore, vessels have been considered as a vital unit. Engineering perfusable functional in vitro has challenging due difficulties designing the connection between rigid macroscale tubes fragile microscale ones. Here, we propose generalizable method engineer "long" vessel network. To form millimeter-scale vessels, fibroblasts were co-cultured with human umbilical vein...
Abstract Rapid bacterial identification remains a critical challenge in infectious disease diagnostics. We developed novel molecular approach to detect and identify wide diversity of pathogens single, simple assay, exploiting the conservation, abundance, rich phylogenetic content ribosomal RNA rapid fluorescent hybridization assay that requires no amplification or enzymology. Of 117 isolates from 64 species across 4 phyla, this identified bacteria with >89% accuracy at level 100% family...
Extracellular response kinase (ERK) is one of the key regulator cell fate, such as proliferation, differentiation and migration. Here, we propose a novel experimental pipeline to learn ERK kinetics by temporal growth factor (GF) stimulation. High signal-to-noise ratio genetically encoded Fluorescence resonance energy transfer (FRET) biosensor enables get large number single-cell activity at each time point, while computer-controlled microfluidics fine-tune Using this platform, observed that...
Airway secretions contain a large number of immune-related cells, e.g., neutrophils, macrophages, and lymphocytes, which can be used as major resource to evaluate variety pulmonary diseases, both for research clinical purposes.However, due the heterogeneous viscous nature patient mucus, there is currently no reliable dissociation method that does not damage host immune cells in airway secretion.In this research, we introduce sample preparation uses inertial microfluidics patient's...
Abstract Stimulation of PC-12 cells with epidermal (EGF) versus nerve (NGF) growth factors (GFs) biases the distribution between transient and sustained single-cell ERK activity states, proliferation differentiation fates within a cell population. We report that fibroblast GF (FGF2) evokes distinct behavior consists gradually changing population transient/sustained signaling states in response to increasing inputs dose response. Temporally-controlled perturbations MAPK dynamics applied using...
Airway secretions contain a large number of immune-related cells, e.g., neutrophils, macrophages, and lymphocytes, which can be used as major resource to evaluate variety pulmonary diseases, both for research clinical purposes.However, due the heterogeneous viscous nature patient mucus, there is currently no reliable dissociation method that does not damage host immune cells in airway secretion.In this research, we introduce sample preparation uses inertial microfluidics patient's...