A5087548457- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Mosquito-borne diseases and control
- Plant Virus Research Studies
- Insect symbiosis and bacterial influences
- Long-Term Effects of COVID-19
- Inflammasome and immune disorders
- Viral Infections and Vectors
- interferon and immune responses
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- RNA modifications and cancer
- HIV Research and Treatment
- Evolution and Genetic Dynamics
- Genomics and Phylogenetic Studies
- RNA Research and Splicing
- Cytomegalovirus and herpesvirus research
- COVID-19 diagnosis using AI
The University of Texas Medical Branch at Galveston
2020-2023
The furin cleavage site (FCS), an unusual feature in the SARS-CoV-2 spike protein, has been spotlighted as a factor key to facilitating infection and pathogenesis by increasing processing. Similarly, QTQTN motif directly upstream of FCS is also for group 2B coronaviruses (CoVs). deletion consistently observed vitro cultured virus stocks some clinical isolates. To determine whether critical replication pathogenesis, we generated mutant deleting (ΔQTQTN). Here, report that attenuates viral...
Similar to other coronaviruses, disruption of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) NSP16 function attenuates viral replication in a type I interferon-dependent manner. In vivo , our results show reduced disease and at late times the hamster lung, but an earlier titer deficit for mutant (dNSP16) upper airway. addition, confirm role IFIT1 also demonstrate necessity IFIT3 mediating dNSP16 attenuation.
Genetic recombination is a tremendous source of intrahost diversity in viruses and critical for their ability to rapidly adapt new environments or fitness challenges. While are routinely characterized using high-throughput sequencing techniques, characterizing the genetic products next-generation data remains challenge. Viral events can be highly diverse variable nature, including simple duplications deletions, more complex such as copy/snap-back recombination, intervirus intersegment...
High-throughput genomics of SARS-CoV-2 is essential to characterize virus evolution and identify adaptations that affect pathogenicity or transmission. While single-nucleotide variations (SNVs) are commonly considered as driving adaption, RNA recombination events delete insert nucleic acid sequences also critical. Whole genome targeting sequencing typically achieved using pairs primers generate cDNA amplicons suitable for next-generation (NGS). However, paired-primer approaches impose...
Abstract Adaptation of viruses to their environments occurs through the acquisition both novel single-nucleotide variants (SNV) and recombination events including insertions, deletions, duplications. The co-occurrence SNVs in individual viral genomes during evolution has been well-described. However, unlike covariation SNVs, studying correlation between with each other or hampered by inherent genetic complexity a lack bioinformatic tools. Here, we expanded our previously reported CoVaMa...
Abstract High-throughput genomics of SARS-CoV-2 is essential to characterize virus evolution and identify adaptations that affect pathogenicity or transmission. While single-nucleotide variations (SNVs) are commonly considered as driving adaption, RNA recombination events delete insert nucleic acid sequences also critical. Whole genome targeting sequencing typically achieved using pairs primers generate cDNA amplicons suitable for Next-Generation Sequencing (NGS). However, paired-primer...
Abstract Genetic recombination is a tremendous source of intra-host diversity in viruses and critical for their ability to rapidly adapt new environments or fitness challenges. While are routinely characterized using high-throughput sequencing techniques, characterizing the genetic products next-generation data remains challenge. Viral events can be highly diverse variable nature, including simple duplications deletions, more complex such as copy/snap-back recombination, inter-virus...
Abstract The furin cleavage site (FCS), an unusual feature in the SARS-CoV-2 spike protein, has been spotlighted as a factor key to facilitating infection and pathogenesis by increasing processing 1,2 . Similarly, QTQTN motif directly upstream of FCS is also for group 2B coronaviruses (CoVs). deletion consistently observed vitro cultured virus stocks some clinical isolates 3 To determine whether critical replication pathogenesis, we generated mutant deleting (ΔQTQTN). Here report that...
Flaviviruses are small RNA viruses that mainly transmitted via arthropod vectors and found in tropic sub-tropical regions. Most infections asymptomatic (90-95%), but symptoms can be as severe hemorrhagic fever encephalitis. One recently emerged flavivirus is Zika virus (ZIKV), which was originally isolated from rhesus monkeys Uganda roughly 70 years ago has spread east, reaching S. America 2015-2016. This outbreak associated with the development of Guillain-Barré syndrome adults microcephaly...
Understanding the molecular basis of innate immune evasion by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an important consideration for designing next wave therapeutics. Here, we investigate role nonstructural protein 16 (NSP16) SARS-CoV-2 in infection and pathogenesis. NSP16, a ribonucleoside 2'-
Abstract Adaptation of viruses to their environments occurs through the acquisition both novel Single-Nucleotide Variants (SNV) and recombination events including insertions, deletions, duplications. The co-occurrence SNVs in individual viral genomes during evolution has been well-described. However, unlike covariation SNVs, studying correlation between with each other or hampered by inherent genetic complexity a lack bioinformatic tools. Here, we expanded our previously reported CoVaMa...
Abstract SARS-CoV-2 is the etiological agent responsible for COVID-19 pandemic. It estimated that only 10 aerosol-borne virus particles are sufficient to establish a secondary infection with SARS-CoV-2. However, dispersal pattern of highly variable and 10– 20% cases up 80% infections. The heterogeneous nature transmission suggests super-spreader events play an important role in viral transmission. Super-spreader occur when single person unusually high number infections due combination...