A5064975604- Neuroscience of respiration and sleep
- Neuroendocrine regulation and behavior
- Sleep and Wakefulness Research
- Neonatal Respiratory Health Research
- Infant Health and Development
- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Heart Rate Variability and Autonomic Control
- Respiratory Support and Mechanisms
- Rare-earth and actinide compounds
- Ion channel regulation and function
- Circadian rhythm and melatonin
- Neonatal and fetal brain pathology
- High-pressure geophysics and materials
- Eicosanoids and Hypertension Pharmacology
- Advanced Thermoelectric Materials and Devices
- Photoreceptor and optogenetics research
- Nicotinic Acetylcholine Receptors Study
- Inhalation and Respiratory Drug Delivery
- Boron and Carbon Nanomaterials Research
- Respiratory and Cough-Related Research
- Smoking Behavior and Cessation
- Magnetic and transport properties of perovskites and related materials
- Zebrafish Biomedical Research Applications
- Iron-based superconductors research
University of California, Los Angeles
2014-2024
Montana Technological University
2024
Salk Institute for Biological Studies
2024
Seattle Children's Hospital
2016
University of Washington
2016
Carnegie Institution for Science
2014
Geophysical Laboratory
2014
BAE Systems (United Kingdom)
2013
Institute of Neurobiology
1999-2012
United States Naval Research Laboratory
1992-2010
The location of neurons generating the rhythm breathing in mammals is unknown. By microsection neonatal rat brainstem vitro, a limited region ventral medulla (the pre-Bötzinger Complex) that contains essential for rhythmogenesis was identified. Rhythm generation eliminated by removal only this region. Medullary slices containing Complex generated respiratory-related oscillations similar to those whole and with voltage-dependent pacemaker-like properties were identified Thus, respiratory...
Neurokinin-1 receptor (NK1R) and mu-opioid (muOR) agonists affected respiratory rhythm when injected directly into the preBötzinger Complex (preBötC), hypothesized site for rhythmogenesis in mammals. These effects were mediated by actions on preBötC rhythmogenic neurons. The distribution of NK1R+ neurons anatomically defined preBötC. Type 1 preBötC, which have properties, expressed both NK1Rs muORs, whereas type 2 only NK1Rs. findings suggest that is a definable anatomic structure with...
Abstract Contraction of the diaphragm is controlled by phrenic motoneurons that receive input from sources are not fully established. Bulbospinal (second‐order) neurons projecting to and propriobulbar (third‐order) these bulbospinal were investigated in rat transsynaptic transport neuroinvasive pseudorabies virus. located predominantly medullary lateral tegmental field two functionally described regions, ventral respiratory group Bötzinger complex. An intervening region, pre‐Bötzinger...
Abstract Efferent and afferent connections of the dorsal ventral respiratory groups in medulla cat were mapped by axonal transport wheat germ agglutinin conjugated to horseradish peroxidase. Injections agglutinin‐horseradish peroxidase into group three principal subdivisions (caudal, rostral, Bötzinger Complex) revealed extensive interconnections between these regions with a limited number other brainstem neuron populations. Major populations efferent projections include parabrachial nuclear...
Inspiration and active expiration are commonly viewed as antagonistic phases of a unitary oscillator that generates respiratory rhythm. This view conflicts with observations we report here in juvenile rats, where by administration fentanyl, selective mu-opiate agonist, induction lung reflexes, separately manipulated the frequency inspirations expirations. Moreover, completely transecting brainstem at caudal end facial nucleus abolished expirations, while rhythmic continued. We hypothesize...
A tiny group of neurons in the brainstem matches breathing rate to level calmness versus arousal.
Data from perinatal and juvenile rodents support our hypothesis that the preBötzinger complex generates inspiratory rhythm retrotrapezoid nucleus-parafacial respiratory group (RTN/pFRG) active expiration (AE). Although role of RTN/pFRG in adulthood is disputed, we hypothesized its rhythmogenicity persists but typically silenced by synaptic inhibition. We show adult anesthetized rats local pharmacological disinhibition or optogenetic excitation can generate AE transforms previously silent...
1. The involvement of excitatory amino acid (EAA) receptors in the generation respiratory rhythm and transmission inspiratory drive to hypoglossal (XII) motoneurons was examined an vitro neonatal rat medullary slice preparation. Slices generated rhythmic activity XII nerves. role EAAs determined by analyzing perturbations network after bath application EAA receptor antagonists or local microinjection into main column neurons ventrolateral medulla (ventral group), particularly pre-Bötzinger...
The breathing motor pattern in mammals originates brainstem networks. Whether pacemaker neurons play an obligatory role remains a key unanswered question. We performed whole-cell recordings the preBötzinger Complex slice preparations from neonatal rodents and tested for activity. observed persistent Na + current ( I NaP )-mediated bursting ∼5% of inspiratory postnatal day 0 (P0)-P5 P8-P10 slices. -mediated was voltage dependent blocked by 20 μ m riluzole (RIL). found Ca 2+ )-dependent 7.5%...
1. An analysis of the spatial and temporal patterns activity neurons respiratory motor-pattern generation system in an vitro neonatal rat brain stem-spinal cord preparation is presented. Impulse discharge spinal cranial moto-neurons as well medulla were analyzed. Patterns motoneuronal characterized at population level from recordings motor-nerve single-cell intracellular recordings. These compared to generated adult mammal vivo establish correspondence between states. 2. The a complex...