A5025168089- HIV Research and Treatment
- Sirtuins and Resveratrol in Medicine
- Histone Deacetylase Inhibitors Research
- Immune Cell Function and Interaction
- Adipose Tissue and Metabolism
- HIV/AIDS drug development and treatment
- Autophagy in Disease and Therapy
- Protein Degradation and Inhibitors
- Epigenetics and DNA Methylation
- Genetics, Aging, and Longevity in Model Organisms
- Diet and metabolism studies
- Biochemical effects in animals
- Ubiquitin and proteasome pathways
- Genomics and Chromatin Dynamics
- Mitochondrial Function and Pathology
- HIV/AIDS Research and Interventions
- Telomeres, Telomerase, and Senescence
- Calcium signaling and nucleotide metabolism
- Cytomegalovirus and herpesvirus research
- T-cell and B-cell Immunology
- PARP inhibition in cancer therapy
- Cancer-related gene regulation
- Nuclear Receptors and Signaling
- RNA Interference and Gene Delivery
- Peptidase Inhibition and Analysis
Buck Institute for Research on Aging
2017-2025
Lifespan
2025
Health & Life (Taiwan)
2025
Gladstone Institutes
2013-2024
University of California, San Francisco
2015-2024
University of Southern California
2023-2024
German United Services Trade Union
2018-2019
Institute of Virology of the Slovak Academy of Sciences
1998-2018
University of Rochester Medical Center
2012
McGill University Health Centre
2012
Stress Protector During prolonged fasting, the oxidation of fatty acids leads to increased accumulation d -β-hydroxybutyrate (βOHB) in bloodstream. Such concentrations βOHB inhibit class I histone deacetylases. Histone acetylation turn influences transcriptional activity at various genes. Shimazu et al. (p. 211 , published online 6 December; see Perspective by Sassone-Corsi ) found that among genes showing transcription animals treated with high were two implicated cellular responses...
The nuclear expression and action of the factor kappa B (NF-kappaB) transcription requires signal-coupled phosphorylation degradation IkappaB inhibitors, which normally bind sequester this pleiotropically active in cytoplasm. subsequent molecular events that regulate termination NF-kappaB remain poorly defined, although activation de novo IkappaBalpha gene by likely plays a key role. Our studies now demonstrate RelA subunit is subject to inducible acetylation acetylated forms interact...
Homologs of the Saccharomyces cerevisiae Sir2 protein, sirtuins, promote longevity in many organisms. Studies sirtuin SIRT3 have so far been limited to cell culture systems. Here, we investigate localization and function vivo. We show that endogenous mouse is a soluble mitochondrial protein. To address relevance regulation energy metabolism, generated phenotypically characterized knockout mice. SIRT3-deficient animals exhibit striking protein hyperacetylation, suggesting major deacetylase....
Hybrid polar compounds (HPCs) have been synthesized that induce terminal differentiation and/or apoptosis in various transformed cells. We previously reported on the development of second-generation HPCs suberoylanilide hydroxamic acid (SAHA) and m -carboxycinnamic bishydroxamide (CBHA) are 2,000-fold more potent inducers a molar basis than prototype HPC hexamethylene bisacetamide (HMBA). Herein we report CBHA SAHA inhibit histone deacetylase 1 (HDAC1) 3 (HDAC3) activity vitro . Treatment...
Protein post-translational modifications (PTMs) at the lysine residue, such as methylation, acetylation, and ubiquitination, are diverse, abundant, dynamic. They play a key role in regulation of diverse cellular physiology. Here we report discovery new type PTM, malonylation (Kmal). Kmal was initially detected by mass spectrometry protein sequence-database searching. The modification comprehensively validated Western blot, tandem MS, high-performance liquid chromatography synthetic peptides,...
We report that human acetyl-CoA synthetase 2 (AceCS2) is a mitochondrial matrix protein. AceCS2 reversibly acetylated at Lys-642 in the active site of enzyme. The sirtuin SIRT3 interacts with and deacetylates both vitro vivo. Deacetylation by activates activity AceCS2. This identifies first substrate protein SIRT3. Our findings show mammalian directly controls metabolic enzyme means reversible lysine acetylation. Because bacterial ortholog AceCS2, called ACS, controlled via deacetylation...