A5102897881- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- Antimicrobial Peptides and Activities
- Single-cell and spatial transcriptomics
- Immune Response and Inflammation
Northwestern University
2019-2020
Robert H. Lurie Comprehensive Cancer Center of Northwestern University
2019
Evanston Hospital
2019
Massachusetts Institute of Technology
2011
The release of cytokines by T cells defines a significant part their functional activity in vivo, and ability to produce multiple has been associated with beneficial immune responses. To date, time-integrated end-point measurements have obscured whether these polyfunctional states arise from the simultaneous or successive cytokines. Here, we used serial, time-dependent, single-cell analysis primary human resolve temporal dynamics cytokine secretion individual after activation ex vivo. We...
Abstract Macrophage-initiated inflammation is tightly regulated to eliminate threats such as infections while suppressing harmful immune activation. However, individual cells’ signaling responses pro-inflammatory cues are heterogeneous, with subpopulations emerging high or low activation states. Here, we use single-cell tracking and dynamical modeling develop validate a revised model for lipopolysaccharide (LPS)-induced macrophage that invokes mechanism term quorum licensing. The results...
Abstract Macrophage-initiated inflammation is tightly regulated to eliminate threats such as infections while suppressing harmful immune activation. However, individual cells’ signaling responses pro-inflammatory cues are heterogeneous; subpopulations emerge with high or low activation states, though why this occurs unknown. To address question, we used single-cell tracking and dynamical modeling develop validate a revised model for macrophage by lipopolysaccharide (LPS) that invokes...