A5036611107- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Cardiac electrophysiology and arrhythmias
- Cellular transport and secretion
- Neurotransmitter Receptor Influence on Behavior
- Advanced Biosensing Techniques and Applications
- Job Satisfaction and Organizational Behavior
- Erythrocyte Function and Pathophysiology
- Nerve injury and regeneration
- Epilepsy research and treatment
- Nanofabrication and Lithography Techniques
- Management Theory and Practice
- Gender Diversity and Inequality
- Receptor Mechanisms and Signaling
- Organ Donation and Transplantation
- Nitric Oxide and Endothelin Effects
- Genetic Neurodegenerative Diseases
- Ion Channels and Receptors
University of California, Davis
2018-2020
Institute of Neurobiology
2018
University of California System
2017
University of Louisville
2016
University of Puerto Rico, Medical Sciences Campus
2011-2016
University of Puerto Rico System
2015
Membrane contacts between endoplasmic reticulum (ER) and plasma membrane (PM), or ER-PM junctions, are ubiquitous in eukaryotic cells platforms for lipid calcium signaling homeostasis. Recent studies have revealed proteins crucial to the formation function of junctions non-neuronal cells, but little is known prominent aspiny regions mammalian brain neurons. The Kv2.1 voltage-gated potassium channel abundantly clustered at neurons first PM protein that functions organize junctions. However,...
The endoplasmic reticulum (ER) and plasma membrane (PM) form junctions crucial to ion lipid signaling homeostasis. Kv2.1 channel is localized at ER-PM in brain neurons unique among PM proteins its ability remodel these specialized contact sites. Here, we show that this function conserved between Kv2.2, which differ their biophysical properties, modulation, cellular expression. Kv2.2 are present sites deficient the actin cytoskeleton, disruption of cytoskeleton affects spatial organization....
Nanobodies (nAbs) are small, minimal antibodies that have distinct attributes make them uniquely suited for certain biomedical research, diagnostic and therapeutic applications. Prominent uses include as intracellular or intrabodies to bind deliver cargo specific proteins and/or subcellular sites within cells, nanoscale immunolabels enhanced tissue penetration improved spatial imaging resolution. Here, we report the generation validation of nAbs against a set prominently expressed at in...
Significance Our data challenge the generally accepted view that Kv2.1 proteins regulate arterial smooth muscle function by regulating their membrane potential. Rather, we discovered plays both conductive and structural roles with opposing functional consequences on myocytes, former predominating in males, latter females. Opening of channels opposes vasoconstriction inducing hyperpolarization. In addition to this function, promotes clustering Ca V 1.2 channels, thereby enhancing 2+ influx...
Abstract The CA1 region of the hippocampus plays a critical role in spatial and contextual memory, has well-established circuitry, function plasticity. In contrast, properties flanking CA2 pyramidal neurons (PNs), important for social lacking CA1-like plasticity, remain relatively understudied. particular, little is known regarding expression voltage-gated K + (Kv) channels contribution these to distinct intrinsic excitability, action potential (AP) waveform, firing patterns...
The neuronal calcium- and voltage-activated BK potassium channel is modulated by ethanol, plays a role in behavioral tolerance vertebrates invertebrates. We examine the influence of temporal parameters alcohol exposure on characteristics molecular ventral striatum, an important component brain reward circuitry. channels striatal neurons C57BL/6J mice exhibited whose duration was function time. After 6 h to 20 m (0.09 mg%) sensitivity suppressed beyond 24 after withdrawal, while 1 or 3...
The Problem Organizational citizenship behaviors (OCBs) are discretionary employee behaviors, such as helping fellow employees or voluntarily promoting the organization, that important contributors to several aspects of performance in organizations. One defining OCBs is these offered freely by employees; they engaged voluntarily. However, for some employees, kinds may be considered expected required part in-role job prescriptions. This case with Soldiers United States Army. Solution In this...
It has been suggested that drug tolerance represents a form of learning and memory, but this not experimentally established at the molecular level. We show component alcohol (channel internalization) from rat hippocampal neurons requires protein synthesis, in common with other forms memory. identify β-catenin as primary necessary protein. Alcohol increases β-catenin, blocking accumulation blocks alcohol-induced internalization these neurons. In transfected HEK293 cells, suppression...
Background The large conductance Ca 2+ ‐ and voltage‐activated K + channel ( BK ) is an important player in molecular behavioral alcohol tolerance. Trafficking surface expression of ion channels contribute to the development addictive behaviors. We have previously reported that internalization a component tolerance ethanol (EtOH). Methods Using primary cultures hippocampal neurons, we combine total internal reflection fluorescence microscopy, electrophysiology, biochemical techniques explore...
Abstract Endoplasmic reticulum (ER) and plasma membrane (PM) form junctions crucial to ion lipid signaling homeostasis. The Kv2.1 channel is unique among PM proteins in organizing ER-PM junctions. Here, we show that this function conserved between Kv2 family members differ their biophysical properties, modulation cellular expression. Manipulation of actin cytoskeleton surrounding affects spatial organization. Kv2-containing overlap with those formed by canonical tethers. junction...
SUMMARY Nanobodies (nAbs) are small, minimal antibodies that have distinct attributes make them uniquely suited for certain biomedical research, diagnostic and therapeutic applications. Prominent uses include as intracellular or intrabodies to bind deliver cargo specific proteins and/or subcellular sites within cells, nanoscale immunolabels enhanced tissue penetration improved spatial imaging resolution. Here, we report the generation validation of nAbs against a set prominently expressed at...