A5057555166- RNA Interference and Gene Delivery
- Cell Image Analysis Techniques
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Genomics and Chromatin Dynamics
- Single-cell and spatial transcriptomics
- Nuclear Receptors and Signaling
- Nerve injury and regeneration
- Phagocytosis and Immune Regulation
- Plant-based Medicinal Research
- Stress Responses and Cortisol
- Plant Genetic and Mutation Studies
- Genetics, Bioinformatics, and Biomedical Research
- Bacterial Identification and Susceptibility Testing
- Computational Drug Discovery Methods
- Neuroscience and Neuropharmacology Research
- Ubiquitin and proteasome pathways
- Chromosomal and Genetic Variations
- DNA Repair Mechanisms
- Cancer-related Molecular Pathways
- Erythrocyte Function and Pathophysiology
- Receptor Mechanisms and Signaling
- PI3K/AKT/mTOR signaling in cancer
- DNA and Nucleic Acid Chemistry
- Bacteriophages and microbial interactions
Second Affiliated Hospital of Zhejiang University
2025
Zhejiang University
2024
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2019-2021
Polish Academy of Sciences
2019-2021
Jagiellonian University
2013-2020
Abstract The human genome is extensively folded into 3-dimensional organization. However, the detailed 3D chromatin folding structures have not been fully visualized due to lack of robust and ultra-resolution imaging capability. Here, we report development an electron microscopy method that combines serial block-face scanning with in situ hybridization (3D-EMISH) visualize at targeted genomic regions (5 × 5 30 nm xyz dimensions) superior current super-resolution by fluorescence light...
The proliferating cell nuclear antigen (PCNA) is post-translationally modified by ubiquitin in yeast and mammalian cells. It widely accepted that mono- polyubiquitinated PCNA involved distinct pathways of DNA postreplication repair. This study showed an interaction between plant the cell. Using different approaches, it was demonstrated Arabidopsis RAD5a ligase post-translational modification PCNA. A detailed analysis properties selected ubiquitin-conjugating enzymes (AtUBC) has shown a...
DNA aptamers are single-stranded oligonucleotides which can form various secondary and tertiary structures. They recognize a broad range of targets ranging from small molecules, such as ions, vitamins, antibiotics, to high molecular weight structures, including enzymes antibodies. extensively studied potential source new pharmaceutical drugs due their inexpensive synthesis, low immunogenicity, specificity. The commonly used aptamer selection procedure is systematic evolution ligands by...
CD44 is a type I transmembrane glycoprotein interacting with number of extracellular components, including hyaluronic acid (HA). CD44-HA axis involved in variety processes, adhesion, migration, differentiation, trafficking, and others. overexpressed several cancers where binding HA induces signal transduction leading to activation antiapoptotic proteins factors linked drug resistance. As such, has been implicated cancer growth, progression, metastasis. It convincingly demonstrated that...
Abstract Exploring the genomic basis of transcriptional programs has been a longstanding research focus. Here, we report high-throughput single-cell tri-omic method to capture ch romatin ccessibility, interaction, and R NA simultaneously (ChAIR). After validating in cultured cells, applied ChAIR brain cells across mouse lifespan delineated concerted dynamics 3D-epigenomic architecture transcription during maturation aging. Particularly, ultra-long chromatin megacontacts promoter-associated...
The Brain-Derived Neurotrophic Factor is one of the most important trophic proteins in brain. role this growth factor neuronal plasticity, health and disease, has been extensively studied. However, mechanisms epigenetic regulation Bdnf gene expression epilepsy are still elusive. In our previous work, using a rat model activation upon kainate-induced seizures, we observed repositioning alleles from nuclear periphery towards center. This change intranuclear position was associated with...
The detailed architectural examination of the neuronal nuclei in any brain region, using confocal microscopy, requires quantification fluorescent signals three-dimensional stacks images. An essential prerequisite to is segmentation which are typically tightly packed tissue, extreme being hippocampal dentate gyrus (DG), frequently appear overlap due limitations microscope resolution. Segmentation DG a challenging task presence significant amount image artifacts and densely nuclei....
Patogeneza depresji jest wciąż słabo poznana. Badania naukowe postulują, że istotną rolę pełnią nieprawidłowości w przekaźnictwie serotonergicznym, na co wskazuje skuteczność działania leków powodujących zahamowanie wychwytu zwrotnego serotoniny. Farmaceutyki te działają z opóźnieniem, sugeruje ich pośredniczenie zmianach długoterminowych, takich jak zmiany ekspresji genów, czy efekty epigenetyczne. Wiadomo także, rozwój zaangażowane są hormony glukokortykoidowe oraz czynniki neurotroficzne (BDNF).
Abstract Brain-Derived Neurotrophic Factor is one of the most important trophic proteins in brain. The role this growth factor neuronal plasticity, health and disease, has been extensively studied. However, mechanisms epigenetic regulation Bdnf gene expression epilepsy are still elusive. In our previous work, using a rat model activation upon kainate-induced seizures, we observed repositioning alleles from nuclear periphery towards center. This change intranuclear position was associated...
Abstract The human genome is extensively folded into 3-dimensional organization, yet the detailed 3D chromatin folding structures have not been fully visualized due to lack of robust and ultra- resolution imaging capability. Here, we report development a novel electron microscopy method that combines serial block-face scanning with in situ hybridization (3D-EMISH) visualize at targeted genomic regions ultra-resolution (5×5×30 nm xyz dimensions, respectively). We applied 3D-EMISH...