A5068765127- Advanced X-ray Imaging Techniques
- Advanced Electron Microscopy Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Electron and X-Ray Spectroscopy Techniques
- Semiconductor Lasers and Optical Devices
- Semiconductor Quantum Structures and Devices
- Laser-induced spectroscopy and plasma
- Liver Disease Diagnosis and Treatment
- 3D Printing in Biomedical Research
- Crystallography and Radiation Phenomena
- Photonic and Optical Devices
- Laser Design and Applications
- Molecular Biology Techniques and Applications
- Medical Imaging Techniques and Applications
- Photoreceptor and optogenetics research
- Advancements in Photolithography Techniques
- Nanowire Synthesis and Applications
- Pluripotent Stem Cells Research
- Liver physiology and pathology
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced Materials Characterization Techniques
- Microfluidic and Bio-sensing Technologies
- Neuroinflammation and Neurodegeneration Mechanisms
- Ion-surface interactions and analysis
- Neurological Disease Mechanisms and Treatments
Institute for Physics of Microstructures
2020-2024
Privolzhsky Research Medical University
2018-2020
N. I. Lobachevsky State University of Nizhny Novgorod
2018-2020
Biomeshes based on decellularized bovine pericardium (DBP) are widely used in reconstructive surgery due to their wide availability and the attractive biomechanical properties. However, efficacy clinical applications is often affected by uncontrolled immunogenicity proteolytic degradation. To address this issue, we present here vivo multi-parametric imaging analysis of epoxy crosslinked DBPs reveal fate after implantation. We first analyzed structure DBP using scanning electron microscopy...
Induced pluripotent stem cells (iPSC) are a promising tool for personalized cell therapy, in particular, the field of dermatology. Metabolic plasticity iPSC not completely understood due to fact that have mixed mitochondrial phenotype, which still resembles somatic cells. In this study we investigated metabolic changes undergoing differentiation two directions, dermal and epidermal, using two-photon fluorescence microscopy combined with FLIM. Directed into fibroblasts keratinocyte progenitor...
The article is devoted to the development of an EUV microscope using a wavelength 13.84 nm. Due use mirror lens with large numerical aperture, NA = 0.27, and short depth focus, it has been possible carry out z-tomography bio-samples for first time this type microscope. A 3D image was reconstructed, pixel resolution 140 nm obtained. new simple algorithm reconstruction absorption images from z -tomography data proposed that takes into account aberrations point spread function. reduces inverse...
The recent advances achieved in microscopy technology have led to a significant breakthrough biological research. Super-resolution fluorescent now allows us visualize subcellular structures down the pin-pointing of single molecules them, while modern electron has opened new possibilities study protein complexes their native, intracellular environment at near-atomic resolution. Nonetheless, both and remained beset by principal shortcomings: reliance on labeling procedures severe sample volume...
Conventional techniques are insufficient precisely to describe the internal structure, heterogeneous cell populations, and dynamics of biological processes occurring in diseased liver during surgery. There is a need for rapid safe method successful diagnosis disease order plan surgery help avoid postoperative failure. We analyze progression both acute (cholestasis) chronic (fibrosis) pathology using multiphoton microscopy with fluorescence lifetime imaging second-harmonic generation modes...
Abstract The emission spectra of krypton plasma in the range 8 – 14 nm upon excitation a pulsed gas jet by 1.06-mm Nd : YAG laser radiation with pulse energy 0.85 J, duration 5.2 ns, and repetition rate 10 Hz are investigated. spectrum is wide (8 nm) band, peaking at 10.3 nm, which formed series much narrower lines. observed lines identified, fraction converted into 14)-nm band emitted half-space (2 π sr) determined. maximum conversion efficiency found to be 21 %. expected throughputs...
We studied the structural and functional state of liver tissue during regeneration process using multiphoton microscopy in combination with fluorescence lifetime imaging (FLIM) second-harmonic generation microscopy, supplementing our findings a mass spectrometry analysis. The was induced by 30% or 70% partial hepatectomy (PH) rats. FLIM were used to evaluate structure assess metabolic hepatocytes, respectively. As result, there statistically significant increase contribution bound forms...
This paper reports the first observation of quasi-Bragg scattering from collimating Goebel mirrors in a real instrument. On basis experimental data obtained and numerical analysis performed, it is concluded that necessary to take into account effect when constructing devices with multilayer for use X-ray diffractometry spectroscopy, especially small-angle position-sensitive detectors.
A brief description of the concept a soft X-ray microscope for Nanoscopy station is given, which planned to be installed at SKIF fourth-generation synchrotron. The will designed study structure cells and dynamic processes in them with nanometer spatial resolution. It use unique absorption contrast ~15 between carbon-containing structures water spectral range “water transparency window”, λ = 2.3–4.3 nm, eliminates need contrasting fluorophores minimizes doses ionizing radiation absorbed...
AbstractThe mode of the growth InGaAs quantum dots by MOS-hydride epitaxy on GaAs substrates without a deviation and with 2° is selected for laser structures emitting at wavelengths above 1.2 μm room temperature. As result, quantum-dot density 4 × 10^10 cm^–2 achieved. Stimulated emission observed in seven layers wavelength 1.06 liquid-nitrogen The threshold power optical pumping about 5 kW/cm^2.
The paper briefly describes the concept of scanning transmission X-ray microscopy (STXM) and Nanoscope station, which is planned to be installed on 4th generation SKIF synchrotron. microscope designed study internal structure cells dynamic processes in them with nanometer spatial resolution. uses a unique absorption contrast between proteins water, region 15, spectral range "water window", λ=2.3 - 4.3 nm, eliminates need for contrasting fluorophores, minimizes doses absorbed samples...
The changes in cell metabolism can affect the epigenome-modifying enzymes activity during iPSCs differentiation and thus control functional potential of final cell. Therefore, for therapeutic applications, restoration a fully mitochondrial network specific types derived from will be required to support energy other factors. Recently, FLIM method allows study metabolic that accompanying noninvasively without additional labels. In this study, we investigated neural using two-photon...
For soft X-ray microscopy in the approximation of geometrical optics, a theoretical model for formation received image is constructed - relationship found between recorded on detector and 3D distribution sample absorption index using high-aperture mirror objective (NA>0.3) with focus depth close to diffraction limit. The solution inverse problem tomography determining this from data measurements SXR-microscope obtained.
Silicon nitride membranes were experimentally obtained as substrates for biological samples, which are examined using a microscope with an operating wavelength of 13.8 nm. The free-hanging films have size up to 1.5 x mm2, makes it possible select area interest investigation on the sample order tens hundreds microns. mechanical strength satisfies that samples do not tear and withstand transportation. results import-substituting technology manufacture Si3N4 membranes. resulting transparency...
The size and intensity of laser-plasma sources based on pulsed argon gas jets operating at a wavelength 13.84 nm were studied. jet was excited by laser radiation 1064 with pulse duration 4.4 ns, repetition frequency 10 Hz, energy 0.5 J. Two methods forming target studied: using supersonic nozzle capillary. capillary source is commercially available. certified mirror microscope in the extreme ultraviolet region nm. It found that due to possibility supplying higher pressure working inlet,...