Autophagy is a conserved cellular recycling and trafficking pathway in eukaryotic cells has been reported to be important the virulence of number microbial pathogens. Here, we report genome-wide identification characterization autophagy-related genes (ATGs) wheat pathogenic fungus Fusarium graminearum. We identified twenty-eight associated with regulation operation autophagy F. Using targeted gene deletion, generated set 28 isogenic mutants. mutants were classified into two groups by...

SUMMARY Tea plant [ Camellia sinensis (L.) O. Kuntze], as one of the most important commercial crops, frequently suffers from anthracnose caused by Colletotrichum camelliae . The plant‐specific tau (U) class glutathione S ‐transferases (GSTU) participates in ROS homeostasis. Here, we identified a GST gene tea plant, CsGSTU45 , which is induced various stresses, including C. infection, analyzing multiple transcriptomes. plays negative role disease resistance against accumulating H 2 O JA...

Tea plant is one of the most important commercial crops worldwide. The Didymellaceae fungi can cause leaf blight disease tea plant. In this study, 240 isolates were isolated from leaves 10 provinces in China. Combined with multi-locus (ITS, LSU, RPB2 and TUB2 ) phylogenetic analysis morphological characteristics, these identified as 25 species six genera Didymellaceae, including 19 known Didymella coffeae-arabicae , D. pomorum segeticola sinensis Epicoccum catenisporum E. dendrobii draconis...

Tea leaf spot caused by

Highlights● CsJAZ11 interacts with CsMYC2.2 in the nuclear● positively regulates disease resistance to Colletotrichum camelliae tea plants● The expression pattern of CsJAZ11-silenced and CsJAZ11-overexpressing samples shows an expected rhythmAbstractTea anthracnose, caused by camelliae, is one most significant foliar diseases plants, resulting substantial losses production. Jasmonic acid signaling pathway crucial regulating ecological interactions between host plants phytopathogens. In this...

Paxillin is a focal adhesion-associated protein that functions as an adaptor to recruit diverse cytoskeleton and signaling molecules into complex plays crucial role in several pathways mammal cells. However, paxillin-mediated signal are largely unknown phytopathogenic fungi. Previously, Pax1 of Magnaporthe oryzae (MoPax1), paxillin-like protein, has been identified pathogenicity determinant. Here, we report the identification mitogen-activated (MAP) kinase (MAPK) activator, Mka1 M. (MoMka1),...

Abstract The tea plant ( Camellia sinensis ) is widely cultivated worldwide as one of the most popular non‐alcoholic beverage crops. Cladosporium , dominant endophytic genus leaves, possesses a variety biotransformation and metabolic abilities to promote accumulation secondary metabolites. However, species in leaves remain unexplored. In this study, total 79 isolates were obtained from several varieties China. Multilocus phylogenetic analysis morphological characteristics revealed six known...

Photosynthesis plays vital roles in plant growth and development. Stay-green (SGR) proteins are responsible for chlorophyll degradation photosynthetic metabolism. To identify SGR family members determine their potential functions tea plants, we identified cloned three genes. Phylogenetic analysis revealed that the homologs were classified into subfamily (named CsSGR1 CsSGR2) SGRL CsSGRL). Cis-element indicated promoters of CsSGR1, CsSGR2 CsSGRL contained light-, phytohormone- stress-related...

The sorting nexin Atg20/Snx42 plays an important role in autophagy. wheat head blight pathogen Fusarium graminearum contains FgAtg20 protein orthologous to Saccharomyces cerevisiae Atg20/Snx42, but its function remains largely unknown. Here, we report a for regulating morphogenesis and fungal pathogenicity. Cytological observation Western blot analysis revealed that ΔFgAtg20 mutants are defective vacuolar transport proteolysis of GFP-FgAtg8, indicating is required non-selective...

Colletotrichum camelliae is the dominant species causing foliar diseases of tea plants (Camellia sinensis) in China. Transcriptome data and reverse transcription-quantitative PCR (qRT-PCR) analysis have demonstrated that pectate lyase genes C. (CcPELs) were significantly upregulated during infectious development on (cv. Longjing43). To further evaluate biological functions CcPELs, we established a polyethylene glycol (PEG)-mediated protoplast transformation system generated targeted deletion...

Nucleotide binding site, leucine-rich repeat (NBS-LRR) proteins are the main types of disease resistance in plants, which can perceive plant pathogens. Anthracnose, caused by fungus <italic>Colletotrichum camelliae</italic>, is one most severe diseases tea plant. Here, we identified an NBS-LRR-encoding gene, <italic>CsRPM1</italic>, probably conferring to <italic>C. camelliae</italic>. Phylogenetic analysis showed that CsRPM1 was clustered with RPM1 <italic>Arabidopsis</italic> and grouped...

Abstract Colletotrichum fructicola , one of the dominant pathogens isolated from main tea region in China, causes leaf spot mature leaves plants, affecting their growth and yield. Here, we present draft whole‐genome sequence C . strain SX‐6 previously used for morphological transcriptomic analyses. The assembly consists 510 contigs with an estimated genome size 56.8 Mb. A total 15,243 predicted protein‐coding genes were annotated using NR, Swiss‐Prot, KEGG, KOG, TCDB, GO, PHI, DFVF, P450,...

In the rice blast fungus Magnaporthe oryzae, cAMP signalling pathway plays a critical role in regulating leaf surface recognition and initiation of appressorium development. Direct downstream targets are, however, not well-characterised. The MoSom1 protein functions dependent kinase A (cAMP-PKA) is essential for infection-related morphogenesis pathogenicity. this study, we show that mutation putative PKA phosphorylation site its differentiation pathogenicity M. oryzae. Mutation serine 227 by...

The mitochondrial distribution and morphology family 33 gene (MDM33) regulates homeostasis by mediating the fission process in yeast. wheat head blight Fusarium graminearum contains an FgMdm33 protein that is orthologous to Saccharomyces cerevisiae Mdm33, albeit its function remains unknown. We have reported here roles of regulating fungal morphogenesis, morphology, autophagy, apoptosis, pathogenicity. ΔFgmdm33 mutants generated through a homologous recombination strategy this study...

The fungal pathogen Didymella segeticola causes leaf spot and blight on tea plant (Camellia sinensis), leading to production losses affecting quality flavor. Accurate detection quantification of D. growth in leaves are crucial for diagnosing disease severity or evaluating host resistance. In this study, we monitored progression development inoculated with a GFP-expressing strain. By contrast, DNA-based qRT-PCR analysis was employed more convenient maneuverable leaves. This method based the...

Phosphatidylcholine (PC) plays crucial biological roles in eukaryotic cells. In Saccharomyces cerevisiae , apart from phosphatidylethanolamine (PE) methylation pathway, PC is also synthesized via CDP–choline pathway. Phosphocholine cytidylyltransferase Pct1 the rate-limiting enzyme to catalyze conversion phosphocholine this Here, we report identification and functional characterization of an ortholog budding yeast PCT1 Magnaporthe oryzae named MoPCT1 . Targeted gene deletion mutants were...