A5076375720- Chronic Obstructive Pulmonary Disease (COPD) Research
- Trace Elements in Health
- Respiratory Support and Mechanisms
- Drug Transport and Resistance Mechanisms
- Heavy Metal Exposure and Toxicity
- Liver physiology and pathology
- Respiratory and Cough-Related Research
- Iron Metabolism and Disorders
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Asthma and respiratory diseases
- Pleural and Pulmonary Diseases
- Lung Cancer Diagnosis and Treatment
- Metal complexes synthesis and properties
- Pancreatic function and diabetes
- Tracheal and airway disorders
- Organ Transplantation Techniques and Outcomes
- Pediatric health and respiratory diseases
- Wnt/β-catenin signaling in development and cancer
- Immune Response and Inflammation
- Tissue Engineering and Regenerative Medicine
- Ion Channels and Receptors
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Olfactory and Sensory Function Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Oral microbiology and periodontitis research
Himeji Medical Center
2019-2025
Japan Medical Association
2021
Teradata (United Kingdom)
2016-2021
European Society of Intensive Care Medicine
2020
Sawai ManSingh Medical College and Hospital
2019
Kyoto University
2008-2012
Tenri Hospital
2004-2010
Southampton General Hospital
2010
Akita University
1995-2005
University of Miyazaki
2000
<h3>Background:</h3> The association between gastro-oesophageal reflux disease (GORD) and chronic obstructive pulmonary (COPD) exacerbation has so far remained unclear. <h3>Objective:</h3> To prospectively establish the clinical significance of GORD symptoms on exacerbation. <h3>Methods:</h3> 82 patients with COPD 40 age matched controls were enrolled in this study. Symptoms evaluated by a questionnaire using Frequency Scale for (FSSG). Patients surveyed 6 months, episodes identified diary...
Low-attenuation areas assessed by computed tomography reflect the extent of pathological emphysema and correlate with airflow limitation mortality in patients chronic obstructive pulmonary disease. The cumulative size distribution low-attenuation area clusters follows a power law characterized an exponent, D. values D complexity terminal airspace geometry sensitively detect alveolar structural changes. Exacerbations disease have negative impact on lung function prognosis. However,...
Wilson's disease, an autosomal recessive disorder, is characterized by the excessive accumulation of copper in liver. <i>WND</i> (<i>ATP7B</i>) gene, which encodes a putative transporting P-type ATPase, defective patients. To investigate <i>in vivo</i> function WND protein as well its intracellular localization, <i>WND</i>cDNA was introduced to Long-Evans Cinnamon rat, known rodent model for recombinant adenovirus-mediated gene delivery. An immunofluorescent study and subcellular...
Background. Amplification or overexpression of the c-erbB-2 gene have been reported to correlate with poor patient prognosis in human breast, gastric, and ovarian cancer. Recently, product was found be expressed frequently urinary bladder carcinoma. In current study, presence carcinomas compared outcome evaluate whether could identify a subset patients who are destined prognosis. Methods. Immunohistologic study done formaldehyde-fixed paraffin-embedded tissue specimens obtained from 88...
Protein disulfide isomerase in isolated rat hepatocytes was present at a concentration of 7 μg/mg cell protein, representing ~2-fold enrichment compared to hepatic non-parenchymal cells. Though localized mainly microsomal fractions hepatocytes, direct immunofluorescence and surface radioiodination followed by immunoprecipitation revealed the presence Mr 57,000 surface. Electrostatic interaction protein with suggested susceptibility carbonate washing. Metabolic radiolabeling studies also...
Tissue damage can be assessed based on regenerative responses, including progenitor cell proliferation. In the salivary gland, tissue induced by ligation of main ducts leads to disappearance acinar cells and marked proliferation ductal cells. Reopening repopulation within 1 2 weeks, which suggests activation in a damaged state. Because submandibular glands derive from endoderm ectoderm, we investigated possibility presence endodermal We cultured obtained ligated gland identified colonies...
ABSTRACT Oval cells function as compensatory in severe liver injury and are thought to be equivalent stem/progenitor cells. We isolated oval from the of Long–Evans Cinnamon (LEC) rats by isopyknic centrifugation a Percoll gradient. The were gamma–glutamyl transpeptidase (GGTP)–positive, alpha–fetoprotein–positive, cytokeratin (CK) 18– CK 19–positive, but albumin–negative When transplanted liver, they transformed into hepatocytes. To evaluate albumin biosynthesis, we Nagase analbuminemic LEC...
Abstract Although extravasation of neutrophils is a critical step in acute inflammation, the role endothelial cytoskeleton neutrophil transmigration has not been fully investigated. We used an vitro model across monolayer HUVEC cultured on amniotic membrane. Human were allowed to migrate response gradient leukotriene B4 and then number migrated counted microscopically. also followed F-actin myosin filament formation using rhodamine-phalloidin anti-myosin Ab staining. Myosin light chain (MLC)...
A major obstacle in the treatment of ovarian carcinoma is intrinsic/acquired resistance to cisplatin-based chemotherapy. Cu-transporting ATPase (ATP7B) has been reported be associated with cisplatin vitro. However, clinical significance this transporter not previously addressed. Our goal was investigate ATP7B expression and whether its correlates prognosis reduced responsiveness treatment.We retrospectively examined p53 primary association chemotherapeutic effect. Tissues were surgically...
Copper is an essential trace element in prokaryotes and eukaryotes strictly regulated by biological mechanisms. Menkes Wilson diseases are human disorders that arise from disruption of the normal process copper export cytosol to extracellular environment. Recently a gene for disease (WD) (also named ATP7B gene) was cloned. This encodes transporter P-type ATPase. We prepared monoclonal polyclonal anti-(WD protein) antibodies characterized full-length WD protein as well shorter form produced...
Abstract Background Health status, dyspnea and psychological status are important clinical outcomes in chronic obstructive pulmonary disease (COPD). However, forced expiratory volume one second (FEV 1 ) measured by spirometry, the standard measurement of airflow limitation, has only a weak relationship with these COPD. Recently, addition to impulse oscillometry (IOS) measuring lung resistance (R) reactance (X) is increasingly being used assess functional impairment. Methods We aimed identify...
Wilson disease is a genetic disorder of copper metabolism characterized by the toxic accumulation in liver. The ATP7B gene, which encodes transporting P‐type ATPase, defective patients with disease. To investigate function ATP7B, wild type or mutated cDNA was introduced into yeast strain lacking CCC2 gene (Δ ccc2 ), homologue . Wild and H1069Q mutant could rescue Δ , however, N1270S not, reflecting phenotypic variability In addition, containing only sixth binding domain ccc2, indicating its...
Abstract Background Interstitial lung abnormalities (ILAs) on CT may affect the clinical outcomes in patients with chronic obstructive pulmonary disease (COPD), but their quantification remains unestablished. This study examined whether artificial intelligence (AI)-based segmentation could be applied to identify ILAs using two COPD cohorts. Methods were diagnosed visually based Fleischner Society definition. Using an AI-based method, ground-glass opacities, reticulations, and honeycombing...
Background The real-world efficacy of the P2X3 antagonist gefapixant, a new class antitussive for refractory chronic cough, is not yet fully understood. This study aimed to evaluate usage and response gefapixant in patients with cough. Methods multicentre, retrospective involved cough who visited clinics were prescribed gefapixant. Data collected included baseline demographics, characteristics, degree taste disturbance (both assessed by specialists), course after discontinuation, if...
Low body mass index (BMI) is a prognostic factor, and skeletal muscle adiposity may affect mortality irrespective of BMI in patients with chronic obstructive pulmonary disease (COPD). However, the association between healthy life expectancy normal-weight remains unestablished. To examine whether lower chest computed tomography (CT)-assessed erector spinae density (ESMD), which represents antigravity adiposity, associated subsequent loss health-related independence COPD. The ESMD limit normal...
Wilson's disease, an autosomal recessive disorder, is characterized by the excessive accumulation of hepatic copper that results from reduced biliary excretion and disturbed incorporation into ceruloplasmin. The ATP7B gene, responsible for encodes a transporting P‐type ATPase. We previously demonstrated involvement in secretion plasma after introduction Long‐Evans Cinnamon (LEC) rat, rodent model disease. In this study we found increased contents lysosomal fractions bile LEC rats...
3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors, pravastatin (Pr) and simvastatin (Si), suppressed 1,2-dimethylhydrazine (DMH)-induced colon cancer development in female ICR mice. All mice received an i.p. injection of 10 mg DMH/kg body wt once weekly for 15 weeks. Pr was administered at 0.01, 0.005 0.001% levels drinking water, Si 0.01 0.002% the diet. animals had access to or throughout experiments which were terminated weeks 25 30. Histologically most tumors...