A5079292818- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Enzyme Production and Characterization
- Advanced Cellulose Research Studies
- Fermentation and Sensory Analysis
- Glycosylation and Glycoproteins Research
- Catalysis for Biomass Conversion
- Lignin and Wood Chemistry
- Carbohydrate Chemistry and Synthesis
- Enzyme-mediated dye degradation
- Microbial Metabolites in Food Biotechnology
Lund University
2021-2024
The success of establishing bioeconomies replacing current economies based on fossil resources largely depends our ability to degrade recalcitrant lignocellulosic biomass. This study explores the potential employing various enzymes acting synergistically previously pretreated agricultural side streams (corn bran, oat hull, soluble and insoluble bran). Degrees synergy (oligosaccharide yield obtained with enzyme combination divided by sum yields individual enzymes) up 88 were obtained....
Xylan is a major component in the complex network of plant cell wall. consists xylose units connected by β-(1,4) glycosidic bonds, which can be heavily substituted, increasing its degradation recalcitrancy. Such substituents include α-D-glucuronic acid (glucopyranosyluronic acid, GlcAp) or 4-O-methyl GlcAp (MeGlcAp) at O-2 position. Removal these catalyzed α-glucuronidases. Here we report novel two-domain α-glucuronidase from GH family 115, termed FAgu115A, that acts on both polymeric and...
Enzymes' uncharacterised side activities can have significant effects on reaction products and yields. Hence, their identification characterisation are crucial for the development of successful systems. Here, we report presence feruloyl esterase activity in CtXyn5A from Acetivibrio thermocellus, besides its well-known arabinoxylanase activity, first time. Activity analysis enzyme variants mutated catalytic nucleophile, Glu279, confirmed removal all E279A E279L, increased while removing...
Abstract Glucuronic acid (GlcA) is an abundant substituent in hardwood xylan, and it often found its methylated form as methyl glucuronic (MeGlcA). GlcA MeGlcA are sugar acids, bound to the xylose backbone at position O-2, their presence can affect digestibility of polymer. Currently, detection released or from synthetic substrates such pNP-glucuronic be achieved with colorimetric assays, whereas analysis natural xylan more complicated. High performance anion exchange chromatography pulsed...
Uncharacterized side activities of enzymes can have significant negative effects on reaction products and yields. Hence, their identification characterization is crucial for the development successful systems. Here, we report presence feruloyl esterase activity in CtXyn5A from Acetivibrio thermocellus besides its well-known arabinoxylanase first time. Both types appear to be catalysed same active site two subsequential steps. The ferulic acid substituent cleaved off first, followed by...