- Heavy metals in environment
- Environmental Toxicology and Ecotoxicology
- Biosensors and Analytical Detection
- Pesticide and Herbicide Environmental Studies
- Aluminum toxicity and tolerance in plants and animals
- Pharmaceutical and Antibiotic Environmental Impacts
- Plant Micronutrient Interactions and Effects
- Heavy Metals in Plants
- Microfluidic and Bio-sensing Technologies
- Mosquito-borne diseases and control
- Mine drainage and remediation techniques
- Electrochemical Analysis and Applications
- Geochemistry and Elemental Analysis
- Enterobacteriaceae and Cronobacter Research
- Microfluidic and Capillary Electrophoresis Applications
- Clay minerals and soil interactions
- Parasitic Diseases Research and Treatment
- Advanced biosensing and bioanalysis techniques
- Malaria Research and Control
- Water Quality and Pollution Assessment
- Bacterial Identification and Susceptibility Testing
- Electrowetting and Microfluidic Technologies
Technical University of Denmark
2014-2015
University of Lisbon
2014
Instituto Superior Técnico
2012
Algoma University
2012
Instituto Politécnico de Lisboa
2012
Stantec (Canada)
2006-2007
Universidade de São Paulo
2007
University of Guelph
2004-2006
Abstract Dengue is a tropical vector-borne disease without cure or vaccine that progressively spreads into regions with temperate climates. Diagnostic tools amenable to resource-limited settings would be highly valuable for epidemiologic control and containment during outbreaks. Here, we present novel low-cost automated biosensing platform detection of dengue fever biomarker NS1 demonstrate it on spiked in human serum. Magnetic nanoparticles (MNPs) are coated high-affinity monoclonal...
We demonstrate a nanoparticle-based assay for the detection of bacteria causing urinary tract infections in patient samples with total time 4 h. This is significantly shorter than current gold standard, plate culture, which can take several days depending on pathogen. The based padlock probe recognition followed by two cycles rolling circle amplification (RCA) to form DNA coils corresponding target bacterial DNA. readout RCA products optomagnetic measurements specific agglutination DNA-bound...
CORUMBATAÍ RIVER AND MAIN AFFLUENTS.Residues of herbicides from sugarcane were monitored in waters and sediments Corumbataí River tributaries.Ametryne, atrazine, simazine, hexazinone, glyphosate, clomazone detected water samples, with negligible levels ametryne glyphosate sediment samples.The area recharge the Guarani aquifer presented highest triazine levels.The triazines at higher levels, atrazine above Brazil's potability quality standards.Total herbicide some sampling points 13 times...
We demonstrate detection of DNA coils formed from a Vibrio cholerae target at picomolar concentrations using novel optomagnetic approach exploiting the dynamic behavior and optical anisotropy magnetic nanobead (MNB) assemblies. establish that complex second harmonic transmission spectra MNB suspensions measured upon application weak uniaxial AC field correlate well with rotation dynamics individual MNBs. Adding analyte to solution leads formation permanent clusters, namely, suppression...
To improve predictions of phytoavailable metal, the mechanistic bases bioaccumulation and toxicity metals to plants can be integrated into a biotic ligand model (BLM). There are number significant challenges application BLM in soils, including reliable measurements free ion concentrations for interest rhizospheric soil solution, as well other ions, ligands which ions could bind; identification simplest that adequately predict root accumulation, potential more complex models add accuracy...
Abstract The focus of the present study was to investigate potential for Al 3 +,Mg 2 +, and H+ influence Ni + toxicity barley seedlings grown in acidic aqueous solutions assess capacity a two‐site terrestrial biotic ligand model (tBLM) accurately predict 50% effect activities (EA50s). To accomplish these objectives, 48‐h EA50 2+ values were obtained three sets exposures which pH activity 3+ Mg varied. Exposures contained both alone combination with so that compound ion effects could be...
Abstract Development of a terrestrial biotic ligand model (TBLM) for higher plants requires root‐Cu accumulation value that corresponds to the 50% inhibitory concentration (IC50). However, it is not yet known which two previously reported Cubinding ligands on root associated with Cu toxicity. The aim present study was therefore investigate relationship between binding each group and toxicity, so key toxicological site could be identified. To obtain toxicity data were biased by limited supply...
A coupled metal transport and speciation model (TRANSPEC) has been developed for surface aquatic systems that explicitly considers the influence of on fate. The TRANSPEC, which is general to most systems, constructed by sequentially coupling speciation/complexation module (in this application MINEQL+) with fugacity/aquivalence approach fate calculations. This formulation increases mechanistic detail, predictive power, fidelity reality current fugacity-aquivalence models metals estimating...
In the present study, predictive measures for Pb toxicity and Lemna minor were developed from bioassays with 7 surface waters having varied chemistries (0.5-12.5 mg/L dissolved organic carbon, pH of 5.4-8.3, water hardness 8-266 CaCO3 ). As expected based on quality, 10%, 20%, 50% inhibitory concentration (IC10, IC20, IC50, respectively) values expressed as percent net root elongation (%NRE) widely (e.g., IC20s ranging 306 nM to >6920 total Pb), unbounded limited by solubility. considering...
Environment Canada has developed a new 42-d sediment toxicity test method that includes reproduction endpoint with the freshwater amphipod Hyalella azteca. Because of concerns existing standard methodologies, whereby adults are transferred to water-only exposure before release their first brood at day 28, will lead internal contaminant depuration and loss sensitivity, methodology conducts entire in sediment. To demonstrate applicability for assessing chemical-spiked sediment, H. azteca were...