A5004675258- Vector-borne infectious diseases
- Blood properties and coagulation
- Yersinia bacterium, plague, ectoparasites research
- Fibroblast Growth Factor Research
- Mosquito-borne diseases and control
- Zoonotic diseases and public health
- Viral Infections and Vectors
- Genomics and Phylogenetic Studies
- Proteoglycans and glycosaminoglycans research
- Angiogenesis and VEGF in Cancer
- Parasites and Host Interactions
- Leptospirosis research and findings
- RNA and protein synthesis mechanisms
- Platelet Disorders and Treatments
- Bacteriophages and microbial interactions
- Insect symbiosis and bacterial influences
- RNA Research and Splicing
- Cell Adhesion Molecules Research
- Heme Oxygenase-1 and Carbon Monoxide
- Adipokines, Inflammation, and Metabolic Diseases
- Invertebrate Immune Response Mechanisms
- Venous Thromboembolism Diagnosis and Management
- Toxoplasma gondii Research Studies
- Research on Leishmaniasis Studies
- Kruppel-like factors research
The University of Texas Medical Branch at Galveston
2015-2024
University of Rochester
2002-2012
Cardiovascular Institute Hospital
2011
Carestream (United States)
2008
PLA 306 Hospital
2008
Maine Medical Center
2003
Maine Medical Center Research Institute
2003
Fibrin is formed at sites of tissue injury and provides the temporary matrix needed to support initial endothelial cell responses for vessel repair. Basic fibroblast growth factor (bFGF) also acts stimulates similar vascular responses. We have, therefore, investigated whether there are specific interactions between bFGF fibrinogen fibrin that could play a role in coordinating these actions. Binding studies were performed using immobilized on Sepharose beads soluble125I-labeled...
Studies have shown improved survival in cancer patients treated with low molecular weight heparins (LMWHs). Tumors depend on an expanding vasculature, and may affect vessel growth function. We investigated the effect of differing Mr selected endothelial cell properties.Human umbilical vein cells were cultured fibroblast factor-2 Mr. Cell proliferation was assessed by [3H]thymidine incorporation, vascular organization vitro assays. Maximum inhibition 94+/-2% observed 6-kDa LMWH, greater than...
Endothelial cell growth is stimulated by fibroblast factor-2 (FGF-2), and both adhesion proliferation are modulated interactions with fibrinogen fibrin. Previous evidence indicates that FGF-2 binds specifically high affinity to fibrin, suggesting their effects on endothelial cells may be coordinated. In this study, we have, therefore, investigated the ability of bound fibrin stimulate cells. Human umbilical vein were cultured in presence or without fibrinogen, was assessed microscopic...
Abstract Fibrin deposition and exudation of plasma fibrinogen (Fg) have long been recognized as hallmarks inflammation, cardiovascular disease neoplasia. The Fg‐β 15–42 domain binds to the endothelial cell adhesion molecule, VE‐cadherin, promoting proliferation, angiogenesis leukocyte diapedesis. Furthermore, spontaneous blood‐borne lymphatic metastasis some types tumor emboli requires fibrin(ogen); however, molecular mechanisms by which this occurs are poorly understood. We sought determine...
Rickettsia species are obligate intracellular Gram-negative pathogenic bacteria and the etiologic agents of diseases such as Rocky Mountain spotted fever (RMSF), Mediterranean fever, epidemic typhus, murine typhus. Genome sequencing revealed that R. prowazekii has ~25 % non-coding DNA, majority which is thought to be either “junk DNA” or pseudogenes resulting from genomic reduction. These characteristics also define other genomes. Bacterial small RNAs, whose biogenesis predominantly...
Rickettsiae, a diverse group of obligately intracellular gram-negative bacteria, include etiologic agents the spotted fever and typhus groups diseases. Rocky Mountain boutonneuse fever, due to Rickettsia rickettsii R. conorii, respectively, are characterized by widespread infection vascular endothelium, microvascular injury, vasculitis. Cultured human endothelial cells (EC) highly susceptible respond altering expression adhesion molecules, regulatory cytokines, antioxidant enzyme heme...
Rickettsial infections continue to cause serious morbidity and mortality in severe human cases around the world. Host cell adhesion invasion is an essential requisite for intracellular growth, replication, subsequent dissemination of pathogenic rickettsiae. Heparan sulfate proteoglycans [HSPGs] facilitate interactions between fibroblast growth factor(s) their tyrosine kinase receptors resulting receptor dimerization/activation have been implicated bacterial target host cells. In present...
Abstract Small regulatory RNAs comprise critically important modulators of gene expression in bacteria, yet very little is known about their prevalence and functions Rickettsia species. R. conorii , the causative agent Mediterranean spotted fever, a tick-borne pathogen that primarily infects microvascular endothelium humans. We have determined transcriptional landscape during infection H uman M icrovascular E ndothelial C ells (HMECs) by strand-specific RNA sequencing to identify 4...
We have recently reported that fibrinogen and fibrin bind to fibroblast growth factor-2 (FGF-2) potentiate its ability stimulate proliferation of endothelial cells. In the present report, we investigated potential protect FGF-2 from proteolytic degradation. was incubated with trypsin or chymotrypsin in presence absence proteolysis assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) Western blotting. there progressive tryptic degradation FGF-2, but fibrinogen,...
Bcr is a serine/threonine kinase activated by platelet-derived growth factor that highly expressed in the neointima after vascular injury. Here, we demonstrate an important mediator of angiotensin (Ang) II and factor–mediated inflammatory responses smooth muscle cells (VSMCs). Among transcription factors might regulate Ang II–mediated found ligand-mediated peroxisome proliferator-activated receptor (PPAR)γ transcriptional activity was significantly decreased II. increased expression...
Endothelial cell interactions with lipopolysaccharide (LPS) involve both activating and repressing signals resulting in pronounced alterations their transcriptome proteome. Noncoding RNAs are now appreciated as posttranscriptional translational regulators of cellular signaling responses, but expression status roles during endothelial LPS not well understood. We report on the profile long noncoding (lnc) human microvascular cells response to LPS. have identified a total 10,781 8310 lncRNA...
Rocky Mountain spotted fever (RMSF) is a potentially fatal tick-borne disease in people and dogs. RMSF reported the United States several countries North, Central, South America.
Rickettsia conorii, the causative agent of Mediterranean spotted fever, preferentially infects human microvascular endothelium and activates pro-inflammatory innate immune responses as evidenced by enhanced expression secretion cytokines chemokines. Our recent studies reveal that endothelial cells (HMECs) infected with R. conorii also launch 'antiviral' host defence mechanisms typically governed type I interferons. To summarize, HMECs secrete IFN-β to activate STAT1 in an autocrine/paracrine...
Emerging evidence implicates a critically important role for bacterial small RNAs (sRNAs) as post-transcriptional regulators of physiology, metabolism, stress/adaptive responses, and virulence, but the roles sRNAs in pathogenic Rickettsia species remain poorly understood. Here, we report on identification both novel well-known prowazekii, known to cause epidemic typhus humans. RNA sequencing human microvascular endothelial cells (HMECs), preferred targets during rickettsioses, infected with...