A5018888685- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Osteoarthritis Treatment and Mechanisms
- Circular RNAs in diseases
- Advances in Cucurbitaceae Research
- Cocoa and Sweet Potato Agronomy
- Genetic Syndromes and Imprinting
- Autophagy in Disease and Therapy
- Ubiquitin and proteasome pathways
- Chromosomal and Genetic Variations
- Inflammatory mediators and NSAID effects
- Cancer-related gene regulation
- Endoplasmic Reticulum Stress and Disease
- Wnt/β-catenin signaling in development and cancer
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Microbial Metabolism and Applications
Ho Chi Minh City Open University
2018-2025
University of Colorado Boulder
2025
University of East Anglia
2013-2020
Norwich Research Park
2015-2020
MicroRNAs have been shown to function in cartilage development and homeostasis, as well progression of osteoarthritis. The objective the current study was identify microRNAs involved onset or early osteoarthritis characterise their chondrocytes. MicroRNA expression mouse knee joints post-DMM surgery measured over 7 days. Expression miR-29b-3p increased at day 1 regulated opposite direction its potential targets. In a model injury end-stage human OA cartilage, miR-29 family also regulated....
ObjectiveTo use deep sequencing to identify novel microRNAs (miRNAs) in human osteoarthritic cartilage which have a functional role chondrocyte phenotype or function.DesignA small RNA library was prepared from primary chondrocytes using in-house adaptors and analysed by Illumina sequencing. Novel candidate miRNAs were validated northern blot qRT-PCR. Expression measured models. Targets of candidates identified microarray computational analysis, 3′-UTR-luciferase reporter plasmids. Protein...
MicroRNAs have been shown to play a role in cartilage development, homeostasis and breakdown during osteoarthritis. We previously identified miR-3085 humans as chondrocyte-selective microRNA, however it could not be detected by Northern blot. The aim of the current study was prove that is microRNA investigate function signaling pathways relevant Here, we confirm another class small RNA using (1) pre-miR hairpin maturation assay, (2) expression levels Dicer null cell line, (3) Ago2 pulldown....
MiR-3085-3p was shown to play a crucial role in cartilage biology, with potential impacts osteoarthritis (OA). Insight into this miRNA function could be of practical importance for future miRNA-based therapy, however, little is known regarding the biological roles miRNA. The physiologic an individual dictated through its mRNA targets, and as SCIN (scinderin, also adseverin) reported involved chondrocyte differentiation, maturation, phenotype maintenance, study aimed prove direct target...
The miR-144-3p was discovered to upregulate in osteoarthritis. However, the function of this molecule is still poorly understood. Identification microRNA target genes could shed a light on miRNA functions. In project, we aim identify direct miR-144-3p. Potential targets were predicted by bioinformatic algorithms. Expression ADAMTSL3 miRNA-144-3p gain-and- loss- experiment quantified Realtime-PCR. Next, 3’UTR containing miR-144-3p-binding sites subcloned 3’;UTR Firefly luciferase gene...
Bản đồ liên kết phân tử với mật độ chỉ thị cao cần được xác định trước khi thực hiện chọn giống bằng DNA marker trong đó các này có sự chặt tính trạng quan tâm. SSR (Simple Sequence Repeat - Trình tự lặp lại đơn giản) là một dùng để xây dựng bản dồ và thể biệt dạng dị hợp đồng của gene. Mục tiêu nghiên cứu bộ trọng dưa leo điều kiện tối ưu cho phản ứng PCR khuyếch đại từ những này. Thực khai thác in silico dữ liệu SSR. Tối hoá chạy gradient nhiệt mồi Chúng tôi đã 52 leo. Điều bắt cặp 46...