A5020350206- Nanoparticles: synthesis and applications
- Gut microbiota and health
- Stress Responses and Cortisol
- Nanoparticle-Based Drug Delivery
- Click Chemistry and Applications
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Aquaculture disease management and microbiota
- Mast cells and histamine
- Environmental Toxicology and Ecotoxicology
- Carbon and Quantum Dots Applications
- Turtle Biology and Conservation
- Immunotherapy and Immune Responses
- Gold and Silver Nanoparticles Synthesis and Applications
- Graphene and Nanomaterials Applications
- Advanced biosensing and bioanalysis techniques
- Hemostasis and retained surgical items
- Aortic aneurysm repair treatments
- Fish biology, ecology, and behavior
- Electrochemical Analysis and Applications
- Mercury impact and mitigation studies
- Protease and Inhibitor Mechanisms
- Tryptophan and brain disorders
- Blood properties and coagulation
- Infant Nutrition and Health
- Apelin-related biomedical research
University of Calgary
2020-2022
Alberta Children's Hospital
2022
University of Alberta
2013-2021
Health Sciences Centre
2021
National Institute for Nanotechnology
2020
Alberta Hospital Edmonton
2016-2020
University of Guelph
2005-2013
Corticotropin-releasing factor (CRF), urotensin I (UI) and serotonin (5-HT) are generally recognized as key regulators of the anorexigenic stress response in vertebrates, yet proximal effects potential interactions these central messengers on food intake salmonids not known. Moreover, no study to date fishes has compared appetite-suppressing CRF UI using species-specific peptides. Therefore, objectives this were (1) assess individual synthesized rainbow trout (rtCRF), rtUI well 5-HT trout,...
Coated nanoparticles (NPs) will end up in the environment due to their proposed use agricultural applications and may potentially cause toxic effects unique properties. To determine of coated NPs on zebrafish (Danio rerio) development, we tested aqueous poly(acrylic acid) (PAA)-coated metal-oxide including TiO2, ZnO, Fe2O3, CeO2, as well polymer coating alone (nanocapsule). Zebrafish embryos were exposed over a 72 h period at 1, 10, 50, 100, 200, 400, 800, 1200, 1600, 2000 mg/L measure...
SUMMARY To assess whether the brain's monoaminergic and/or corticotropin-releasing factor (CRF) systems may be involved in mediating appetite-suppressing effects of high environmental ammonia levels, we exposed rainbow trout to one four NH4Cl treatments (0, 500, 750 1000 μmol l-1)for 24 or 96 h and monitored changes food intake, brain serotonin (5-HT)and dopamine (DA) activity, CRF urotensin I (UI) mRNA plasma cortisol levels. Food intake decreased a dose-dependent manner after exposure...
Nanomaterials in biological solutions are known to interact with proteins and have been documented affect protein function, such as enzyme activity. Understanding the interactions of nanoparticles components at molecular level will allow for rational designs nanomaterials use medical technologies. Here we present first detailed mechanics model functionalized gold nanoparticle (NP) interacting an ( l ‐lactate dehydrogenase (LDH) enzyme). Molecular dynamics (MD) simulations response LDH NP...
Background. Early-life disruptions to the gut microbiome and stress-axis significantly influence development of immune, neuroendocrine, other physiological systems. However, precise microbial species pathways mediating these effects remain poorly characterized. Using a murine model, we investigated individual combined early-life antibiotic exposure chronic stress on microbiota composition, short-chain fatty acid (SCFA) production, hypothalamic-pituitary-adrenal (HPA) axis activity, systemic,...
Exposure effects from polyacrylic acid (PAA) metal-oxide nanoparticles (TiO2, CeO2, Fe2O3, ZnO) on fish neutrophil viability and effector functions (degranulation, respiratory burst, inflammatory gene expression) were investigated using primary kidney goldfish (Carassius auratus L.) neutrophils as a model. Several studies have reported cytotoxic of NPs but there are limited reports their potential to perturb the innate immune system aquatic organisms. PAA-TiO2 significantly decreased in time...
Previous reports have shown that nanoparticles (NPs) can both enhance and suppress immune effector functions; however the mechanisms dictate these responses are still unclear. Here, effects of polyacrylic acid (PAA) functionalized metal‐oxide NP investigated on RBL‐2H3 (representative mammalian granulocyte‐like cell line) viability, cellular degranulation, immunoglobulin E (IgE) receptor binding, signaling pathways related to function. The increasing development PAA‐NPs as pesticide...
In this study, the effects of rosette nanotube (RNT) exposure on immune cell viability and function were investigated in vitro using rat basophilic leukemia (RBL)-2H3 line. RBL-2H3 was decreased a dose- time-dependent manner after lysine-functionalized RNT (K-RNT) exposure. addition, K-RNTs had significant effect degranulation. When K-RNT concurrent with IgE sensitization, 50 100 mg l(-1) elicited heightened degranulatory response compared alone. Exposure to also caused degranulation cells...
Nanoparticles (NPs) that are exposed to blood coated with an assortment of proteins establish their biological identity by forming the interface between NP and cells tissues body. The relevance this protein corona is often overlooked during toxicological assessments NPs. However, accurate interpretation outcomes following exposure NPs, including activation coagulation, opsonization pathogens, cellular phagocytosis, must take adsorbed proteome into account. In study, we examined coronas on...
Fish lymphocytes are a sensitive model to examine the effects of different surface functionalizations on rosette nanotube toxicity.
Cane toad iliac arteries were perfused<italic>ex vivo</italic>with polyacrylic acid coated nanoparticles. Nanoparticle uptake was observed in both endothelial and vascular smooth muscle cells, eliciting cell apoptosis diminished relaxation.
Development of elastin-like polypeptide (ELP) biomaterials is widespread, but information critical for clinical deployment limited, with biocompatibility studies focused on a narrow cross-section ELP sequences. Macrophages can impair biomaterial systems by degrading or isolating the and activating additional immune functions. Their phagocytic response will reveal early nanoparticles (NPs). This study examines that response, induced adsorbed protein corona, as function guest amino acid, chain...
We examined goldfish immunotoxicological responses to intravenously injected PAA-TiO<sub>2</sub> NPs by determining organ accumulation, depuration, tissue damage, and immune responsiveness in the kidneys, spleen, isolated primary kidney neutrophils.
The design and fabrication of Si-based multi-functional nanomaterials for biological biomedical applications is an active area research. potential benefits using are not only due to their size/surface-dependent optical responses but also the high biocompatibility low-toxicity silicon itself. Combining these characteristics with magnetic properties Fe 3 O 4 nanoparticles (NPs) multiplies options available real-world applications. In current study, biocompatible magnetofluorescent nano-hybrids...
<p>The design and fabrication of Si-based multi-functional nanomaterials for biological biomedical applications is an active area research. The potential benefits using are not only due to their size/surface-dependent optical responses but also the high biocompatibility low-toxicity silicon itself. Combining these characteristics with magnetic properties Fe<sub>3</sub>O<sub>4</sub> nanoparticles (NPs) multiplies options available real-world applications. In...