A5019006688- ATP Synthase and ATPases Research
- Drug Transport and Resistance Mechanisms
- Ion Transport and Channel Regulation
- Trace Elements in Health
- RNA and protein synthesis mechanisms
- Endoplasmic Reticulum Stress and Disease
- Cellular transport and secretion
- Ion channel regulation and function
- Fungal and yeast genetics research
- Pancreatic function and diabetes
- Protein Structure and Dynamics
Instituto de Química y Fisicoquímica Biológicas
2020-2022
Consejo Nacional de Investigaciones Científicas y Técnicas
2020-2022
University of Buenos Aires
2020-2022
P5 ATPases are eukaryotic pumps important for cellular metal ion, lipid and protein homeostasis; however, their transported substrate, if any, remains to be identified. Ca2+ was proposed act as a ligand of because it decreases the level phosphoenzyme Spf1p P5A ATPase from Saccharomyces cerevisiae. Repeating previous purification protocols, we obtained purified preparation that close homogeneity exhibited ATP hydrolytic activity stimulated by addition CaCl2. Strikingly, catalytically dead...
The yeast Spf1p protein is a primary transporter that belongs to group 5 of the large family P-ATPases. Loss function produces ER stress with alterations metal ion and sterol homeostasis folding, glycosylation membrane insertion. amino acid sequence shows characteristic P-ATPase domains A, N, P transmembrane segments M1-M10. In addition, exhibits unique structures at its N-terminus (N-T region), including two putative additional domains, insertion connecting domain segment M5 (D region)....
The plasma membrane Ca 2+ pumps (PMCA) are P-ATPases that control signaling and homeostasis by transporting out of the eukaryotic cell. Humans have four genes code for PMCA isoforms (PMCA1-4). A large diversity is generated alternative mRNA splicing at sites C. different expressed in a cell-type developmental-specific manner exhibit differential sensitivity to great number regulatory mechanisms. PMCA4 has two splice variants, forms “x” “z”. While PMCA4x ubiquitously relatively well-studied,...