A5091148822- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Hepatitis C virus research
- Immune Response and Inflammation
- Cholesterol and Lipid Metabolism
- Hepatitis B Virus Studies
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Telomeres, Telomerase, and Senescence
- Viral Infections and Immunology Research
- Pneumonia and Respiratory Infections
- Ovarian function and disorders
- Animal Virus Infections Studies
- RNA Interference and Gene Delivery
- Atherosclerosis and Cardiovascular Diseases
- Reproductive biology and impacts on aquatic species
- Seed Germination and Physiology
- Reproductive System and Pregnancy
- Genetics, Aging, and Longevity in Model Organisms
- Reproductive Health and Technologies
- Immunotherapy and Immune Responses
- Amyotrophic Lateral Sclerosis Research
- T-cell and B-cell Immunology
- Hereditary Neurological Disorders
- Neurogenetic and Muscular Disorders Research
Université Clermont Auvergne
2018-2024
Inserm
2002-2024
Centre Hospitalier Universitaire de Clermont-Ferrand
2022
Imagerie Moléculaire et Stratégies Théranostiques
2020-2021
Centre National de la Recherche Scientifique
2018
Université Claude Bernard Lyon 1
2008-2012
Fondation Mérieux
2012
Structure Fédérative de Recherche Biosciences
2008
Identification of new techniques to express proteins into mammal cells is particular interest for both research and medical purposes. The present study describes the use engineered vesicles deliver exogenous human cells. We show that overexpression spike glycoprotein vesicular stomatitis virus (VSV-G) in induces release fusogenic named gesicles. Biochemical functional studies revealed gesicles incorporated from producer could them recipient This protein-transduction method allows direct...
Can a discriminant threshold be determined for human sperm DNA oxidation? A was found with 65.8% of 8-hydroxy-2'-deoxyguanosine (8-OHdG)-positive cells and mean intensity fluorescence (MIF) 552 arbitrary units. Oxidative stress is known to interfere quality fertilizing capacity. However, current practice does not include the routine determination oxidative damage in spermatozoa; optimized consensus protocols are lacking no thresholds normality have been established. Intra- inter-method...
BackgroundLack of protective antibodies and inefficient cytotoxic responses are characteristics chronic hepatitis C infection. A defect in dendritic cell (DC) function has thus been suspected, but this remains a controversial issue.Methods FindingsHere we show that monocyte-derived DC (MoDC) from chronically-infected patients can mature response to TLR1/2, TLR2/6 or TLR3 ligands. In contrast, when stimulated with the TLR4 ligand LPS, MoDC profound inducing IFNγ secretion by allogeneic T...
An unexplained increase in the incidence of parapneumonic empyema (PPE) pneumonia cases has been reported recent years. The present study investigated genetic and biological specifications new isolates torque teno mini virus (TTMV) detected pleural effusion samples from children hospitalised for severe with PPE. A pathogen discovery protocol was applied undiagnosed led to identification three TTMV (TTMV-LY). Isolated TTMV-LY genomes were transfected into A549 human embryonic kidney 293T...
Correlations were reported between sperm telomere length (STL) and male fertility, DNA fragmentation, oxidation. Sperm freezing is widely used for assisted reproductive techniques, fertility preservation, donation. However, its impact on STL remains unknown. For this study, semen surplus from patients who underwent routine analysis used. The of slow was analyzed by performing qPCR before after freezing. populations with different evaluated using Q-FISH. relationship oxidation, assessed in...
Abstract Cryopreservation of in vitro matured oocytes is still considered as an experimental alternative to mature oocyte vitrification after ovarian stimulation. Here, we investigated whether rescue-IVM should be performed before or vitrification. For this, 101 immature (germinal vesicle stage) from women undergoing ICSI were used. Oocytes divided into three groups: freshly (IVM), subsequently vitrified (IVM + VIT) and vitrified/warmed GV then (VIT IVM). Oocyte maturation rates kinetics...
Pneumonia is caused by respiratory bacteria and/or viruses. Little known if co-infections are an aggravating factor in hospitalised children with severe pneumonia. We studied the impact of pathogens on severity Between 2007 and 2009, 52 a well-documented diagnosis community-acquired pneumonia (CAP), or without parapneumonic empyema (PPE), were enrolled study. The patients classified into 2 groups: CAP + PPE (n = 28) 24). identification viruses nasopharyngeal aspirates pleural effusion...
Abstract Background Although widely used, slow freezing considerably modifies the functions of human spermatozoa. Cryopreservation induces nuclear sperm alterations and cryo-capacitation, reducing chances pregnancy. Hypotaurine is naturally present in male female genital tracts has capacitating, osmolytic anti-oxidant properties. The analysis were performed on surplus semen men with normal ( n = 19) or abnormal 14) parameters. Spermatozoa selected by density gradient centrifugation before...