Abstract Stimuli‐responsive materials have the potential to enable generation of new bioinspired devices with unique physicochemical properties and cell‐instructive ability. Enhancing biocompatibility while simplifying production methodologies, as well enabling creation complex constructs, i.e., via 3D (bio)printing technologies, remains key challenge in field. Here, a novel method is presented biofabricate cellularized anisotropic hybrid hydrogel through mild biocompatible process driven by...

Abstract Morphogenesis, a complex process, ubiquitous in developmental biology and many pathologies, is based on self-patterning of cells. Spatial patterns cells, organoids, or inorganic particles can be forced demand using acoustic surface standing waves, such as the Faraday waves. This technology allows tuning parameters (sound frequency, amplitude, chamber shape) under contactless, fast mild culture conditions, for morphologically relevant tissue generation. We call this method Sound...

The native structure of tissues and organs is characterized by a hierarchical architecture, where various cell types extracellular matrix components are closely interconnected. precise organization these entities crucial for ensuring the proper functionality organs. Therefore, engineering spatial complexity living systems essential not only to mimic in vivo architecture but also govern microenvironments embedded cells reside. Bioassembly an innovative toolset vitro modeling regenerative...

Herein we show an accessible technique based on Faraday waves that assist the rapid assembly of osteoinductive β-Tricalcium phosphate (β-TCP) particles as well human osteoblast pre-assembled in spheroids. The hydrodynamic forces originating at 'seabed' chamber can be used to tightly aggregate inorganic and biological entities packing densities resemble those native tissues. Additionally, following a layer-by-layer procedure, centimeter scaled three-dimensional cellularized constructs have...

Herein, the fabrication of multi‐responsive and hierarchically organized nanomaterial using core‐shell SrF 2 upconverting nanoparticles, doped with Yb 3+ , Tm Nd incorporated into gelatin methacryloyl matrix, is reported. Upon 800 nm excitation, deep monitoring 3D‐printed constructs demonstrated. Addition magnetic self‐assembly iron oxide nanoparticles within hydrogel provides anisotropic structuration from nano‐ to macro‐scale responsiveness permitting remote manipulation. The present study...

Abstract During developmental processes, cells frequently condense along a preferred axis, creating columnar arrangements—a pivotal step in shaping elongating tissue structures and facilitating gradual building of complexity. Despite advances biofabrication technology that has allowed researchers to recreate these axial arrangements vitro 3D culture, maintaining patterns for periods cultivation beyond 7 days proven challenging, given cells' tendency exhibit random migratory patterns. In this...

Two peaks, K1 and K2, in place of the Kě one, are found by low amplitude internal friction measurements on annealed Al polycrystals. These K-peaks probably depend dislocation damping since they appear also with bent single crystals having only dislocations, free or polygonization walls. This view is supported occurrence modulus defects corresponding to non-deformed crystals, dislocations. The activation energies two peaks comparable those characterizing creep above room temperature. Zwei...

Abstract Stimuli-responsive nanomaterials have the prospective to enable fabrication of new extracellular matrix-like substrates with unique structures and cell-instructive capabilities. The development biocompatible collagen on-demand ordered architectures is an open challenge since it well-known that directionality fibres affects important cell behaviour, such as proliferation, differentiation, ultimately, tissue regeneration. Here, easy cheap approach fabricate anisotropic collagen-based...

Optically trapping red blood cells allows for the exploration of their biophysical properties, which are affected in many diseases. However, because nonspherical shape, numerical calculation optical forces is slow, limiting range situations that can be explored. Here we train a neural network improves both accuracy and speed employ it to simulate motion cell under different beam configurations. We found by fixing two beams controlling position third, possible control tilting cell. anticipate...

Slight deformation of recrystallized S.A.P. sheets causes some internal friction peaks to appear above room temperature. One these corresponds the peak characteristic as-rolled which disappears after recrystallization. Based on observations a dislocation damping interpretation is discussed with reference dragging action connected diffusive motion jogs. Schwache Deformation von rekristallisierten “S.A.P.”-Blechen führt zur Ausbildung einiger Maxima der inneren Reibung oberhalb Raumtemperatur....

In article number 1804647, Tiziano Serra and co-workers report a novel method to biofabricate anisotropic nanocomposite hydrogels through mild biocompatible process driven by multiple external stimuli: magnetic field, temperature, light. The functionality of this stimuli-responsive hydrogel was studied creating i) 3D cell-instructive platforms for in vitro morphogenesis, ii) printable magneto-responsive ink fabricating small-scale bio-inspired soft-robots.

Red blood cells (RBCs) or erythrocytes are essential for oxygenating the peripherical tissue in human body. Impairment of their physical properties may lead to severe diseases. Optical tweezers have experiments been shown be a powerful tool assessing biochemical and biophysical RBCs. Despite this success there has little theoretical work investigating stability optical tweezers. In paper we report numerical study trapping RBCs healthy, native biconcave disk conformation using ray optics...

Abstract Discogenic pain is associated with deep nerve ingrowth in annulus fibrosus tissue (AF) of intervertebral disc (IVD). To model AF ingrowth, primary bovine dorsal root ganglion (DRG) micro-scale units are spatially organized around an explant by mild hydrodynamic forces within a collagen matrix. This results densely packed multicellular system mimicking the native DRG morphology and controlled AF-neuron distance. Such organization essential to evolve populational-level cellular...

Multifunctional HydrogelsIn article number 1900105, Adolfo Speghini, Tiziano Serra, and co-workers report the fabrication of a 3D printed multi-responsive hierarchically organized material for deep monitoring remote manipulation. This light magnetic field responsive hydrogel holds promise generation advanced bionic devices.

Osteoarthritis (OA) is a degenerative joint disease affecting millions worldwide. Early detection of OA and monitoring its progression essential for effective treatment preventing irreversible damage. Although sensors have emerged as promising tool analytes in patients, their application the state pathology currently restricted to specific fields (such diabetes). In this study, we present development an optical sensor system real-time inflammation based on measurement nitric oxide (NO),...

Spatial cell organization and biofabrication of microcapillary networks in vitro has a great potential tissue engineering regenerative medicine. This study explores the impact local density enhancement achieved through an innovative sound-based patterning on formation their proteomic profile. Human umbilical vein endothelial cells (HUVEC) human pericytes from placenta (hPC-PL) were mixed fibrin suspension. The mild effect sound-induced hydrodynamic forces condensed into architected...

Magnetic soft robots have emerged as promising tools in biomedicine due to their wireless actuation, rapid response, and compatibility with biological systems. Despite the various innovative strategies proposed for fabrication, they suffer from complex processes limited cellular compatibility. Therefore, there is an urgent need explore alternative approaches that are fast, mild, highly tunable. Herein, a novel fabrication strategy contactless assembly of soft‐robots introduced first time....

It is well known that single crystals of metals do not show overheating. This may be explained by means heterophase fluctuations homogeneously catalyzed at the interface between dislocation cores and surrounding bulk material, on assumption are preferential sites premelting. Es ist gut bekannt, daß Metalleinkristalle keine Überheizung zeigen. Dies läßt sich erklären mit Heterophasenfluktuationen, die homogen an der Grenzfläche zwischen den Versetzungskernen und dem umgebenden Volumenmaterial...

Herein we show an accessible technique based on Faraday waves that assist the rapid assembly osteoinductive β-TCP particles as well human osteoblast pre-assembled in spheroids. The hydrodynamic forces originating at 'seabed' of chamber can be used to tightly aggregate inorganic and biological entities packing densities resemble those native tissues. Additionally, following a layer-by-layer procedure, centimeter scaled three-dimensional cellularised constructs have been fabricated. We showed...

The interface free energies per unit area of small liquid clusters, embedded in a close packed matrix are evaluated at temperature, T, near the melting Tm, by means hard sphere model on basis simple geometrical and thermodynamical considerations. For clusters containing 13 19 atoms with 12 18 surface atoms, respectively, one finds higher than energy area, σ0, planar liquid—solid (bulk energy). Auf der Grundlage von einfachen geometrischen und thermodynamischen Betrachtungen werden mittels...

Abstract The solid-liquid interfacial free energy per unit area of metals has been found to increase with decrease temperature. Consequently the corresponding temperature coefficient is negative.