- Electrochemical sensors and biosensors
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Electrochemical Analysis and Applications
- Advanced Biosensing Techniques and Applications
- Analytical Chemistry and Sensors
- Anesthesia and Neurotoxicity Research
- Microfluidic and Bio-sensing Technologies
- Anodic Oxide Films and Nanostructures
- Food Allergy and Anaphylaxis Research
- Advanced Nanomaterials in Catalysis
- Health, Environment, Cognitive Aging
- Mercury impact and mitigation studies
- Nanopore and Nanochannel Transport Studies
- GDF15 and Related Biomarkers
- Systemic Sclerosis and Related Diseases
- Quantum Dots Synthesis And Properties
- Advanced Drug Delivery Systems
Universidad de Oviedo
2016-2022
University of Florence
2020
In this work, bifunctional core@shell Au@Pt/Au NPs are presented as novel tags for electrochemical immunosensing. were synthesized following a chemical route based on successive metal depositions and galvanic replacement reactions from the starting AuNPs. Au protuberances growth surface of Au@Pt allowed their easy bioconjugation with antibodies, while high catalytic Pt area was approached sensitive detection through electrocatalyzed water oxidation reaction (WOR) at neutral pH. Moreover,...
Nowadays, food allergy is a very important health issue, causing adverse reactions of the immune system when exposed to different allergens present in food. Because this, development point-of-use devices using miniaturized, user-friendly, and low-cost instrumentation has become outstanding importance. According electrochemical aptasensors have been demonstrated as useful tools quantify broad variety targets. In this work, we develop simple methodology for determination β-lactoglobulin (β-LG)...
This lab experiment describes a complete method to fabricate an enzymatic glucose electroanalytical biosensor by students. Using miniaturized and disposable screen-printed electrodes (SPEs), students learn how use them as transducers understand the importance SPEs have acquired in sensor development during last years. Students can also revise concepts related assays, with oxidase horseradish peroxidase involved subsequent reactions. Moreover, they trends that current analytical chemistry...
Chronic wounds represent an important healthcare challenge in developed countries, being wound infection a serious complication with significant impact on patients' life conditions. However, there is lack of methods allowing early diagnosis and right decision making for correct treatment. In this context, we propose novel methodology the electrical monitoring biomarkers chronic exudates, using nanoporous alumina membranes. Lysozyme, enzyme produced by human immune system indicating...
Aims: There are several candidate biomarkers for AD and PD which differ in sensitivity, specificity, cost-effectiveness, invasiveness, logistical technical demands. This study is aimed to test whether plasma concentration of unfolded p53 may help discriminate among the neurodegenerative processes occurring Mild Cognitive Impairment, Alzheimer’s disease Parkinson’s disease. Method: An electrochemical immunosensor was used measure samples 20 Impairment (13 males/7 females; mean age...
An electrochemical biosensor for creatinine determination in a drop of whole human blood was developed and applied to the real clinical samples. It is based on modification dual carbon working electrode with combination three enzymes: amidohydrolase (CNN), creatine amidinohydrolase (CRN) sarcosine oxidase (SOX). Electrochemical transduction performed using horseradish peroxidase (HRP) potassium hexacyanoferrate(II) as mediator. A enough carry out measurements by differential...
An electroanalytical platform capable to take and dilute the sample has been designed in order fully integrate different steps of analytical process only one device. The concept is based on addition glass-fiber pads for sampling diluting an electrochemical cell combining a paper-based working electrode with low-cost connector headers as counter reference electrodes. In demonstrate feasibility this all-in-one biosensing applications, enzymatic sensor glucose determination (requiring potential...
Analytical Chemistry is nowadays one of the branches that has changed enormously due to advances in technology and trends it follows. Currently, development low-cost devices are, at same time, easy-to use dispose, produce fast reliable responses eminent significance. Electrochemical biosensors fit perfectly with these requirements, as do paper-based devices. Here we present an enzymatic biosensor using a simple single-use device which carbon ink deposited hydrophilic area delimited by wax...
The increasing demand for fast and on-site information has generated great interest in developing simple portable analytical devices that provide reliable responses. Electroanalytical fit perfectly with these purposes because of their ease use, low cost facility miniaturization. Moreover, the growing construction do-it-yourself electronic spread use common mass-produced materials development devices. In this context, it is presented here gold-plated pins, from standard connections,...