A5003966461- Nicotinic Acetylcholine Receptors Study
- Receptor Mechanisms and Signaling
- PARP inhibition in cancer therapy
- Vagus Nerve Stimulation Research
- Toxin Mechanisms and Immunotoxins
- Inflammatory mediators and NSAID effects
- Calcium signaling and nucleotide metabolism
- Estrogen and related hormone effects
- Sphingolipid Metabolism and Signaling
- Lipid Membrane Structure and Behavior
- Eicosanoids and Hypertension Pharmacology
- Integrated Circuits and Semiconductor Failure Analysis
- Cancer, Hypoxia, and Metabolism
- Cellular transport and secretion
- Electrostatic Discharge in Electronics
- Neuropeptides and Animal Physiology
- Neuroscience and Neuropharmacology Research
- Advanced biosensing and bioanalysis techniques
- Immune Response and Inflammation
- Wnt/β-catenin signaling in development and cancer
- Neurogenesis and neuroplasticity mechanisms
- RNA Interference and Gene Delivery
- Cancer, Stress, Anesthesia, and Immune Response
- Synthesis and Biological Evaluation
- Molecular Communication and Nanonetworks
Doshisha Women's College of Liberal Arts
2016-2024
Pharmac
2020-2021
National Heart Lung and Blood Institute
2012-2019
National Institutes of Health
2012-2014
The University of Tokyo
2010-2011
Chiba University
2008-2010
Osaka City University
2006
Poly(ADP-ribose) polymerase 1 (PARP1) is a nuclear protein that activated by binding to DNA lesions and catalyzes poly(ADP-ribosyl)ation of acceptor proteins, including PARP1 itself, recruit repair machinery lesions. When excessive damage occurs, poly(ADP-ribose) (PAR) produced translocated the cytoplasm, changing activity localization cytoplasmic e.g., apoptosis-inducing factor (AIF), hexokinase, resulting in cell death. This cascade, termed parthanatos, caspase-independent programmed death...
Significance Poly (ADP-ribose) (PAR) participates in initiating parthanatos, cell death mediated by apoptosis-inducing factor (AIF), following genotoxic stress. PAR translocation from the nucleus to cytoplasm is an essential step for AIF-mediated death. Here, we report that poly glycohydrolase (PARG) enhances releasing protein-free fragments nuclear acceptor proteins. ADP-ribosyl-acceptor hydrolase (ARH) 3 lowers levels and cytoplasm, preventing then parthanatos. Elucidation of sequential...
It is now apparent that immune cells express a functional cholinergic system and α7 nicotinic acetylcholine receptors (α7 nAChRs) are involved in regulating T cell differentiation the synthesis of antigen-specific antibodies proinflammatory cytokines. Here, we investigated specific function nAChRs on antigen presenting (APCs) by testing effect GTS-21, selective nAChR agonist, CD4+ from ovalbumin (OVA)-specific TCR transgenic DO11.10 mice activated with OVA or peptide323-339 (OVAp). GTS-21...
Poly(ADP-ribosyl)ation refers to the covalent attachment of ADP-ribose protein, generating branched, long chains moieties, known as poly(ADP-ribose) (PAR). Poly(ADP-ribose) polymerase 1 (PARP1) is main and acceptor PAR in response DNA damage. Excessive intracellular accumulation due PARP1 activation leads cell death a pathway parthanatos. degradation mainly controlled by glycohydrolase (PARG) ADP-ribose-acceptor hydrolase 3 (ARH3). Our previous results demonstrated that ARH3 confers...
Abstract Sphingosine kinases (SphKs) and ceramide kinase (CerK) phosphorylate sphingosine to sphingosine‐1‐phosphate (S1P) ceramide‐1‐phosphate (C1P), respectively. S1P C1P are bioactive lipids that regulate cell fate/function human health/diseases. The translocation activity of SphK1 regulated by its phosphorylation Ser 225 anionic such as phosphatidic acid phosphatidylserine. However, the roles another lipid on functions have not yet been elucidated, thus, we here investigated regulation...
Loewi’s discovery of acetylcholine (ACh) release from the frog vagus nerve and by Dale Dudley ACh in ox spleen led to demonstration chemical transmission impulses. is now well-known function as a neurotransmitter. However, advances techniques for detection have its many lifeforms lacking nervous system, including eubacteria, archaea, fungi, plants. Notably, mRNAs encoding choline acetyltransferase muscarinic nicotinic receptors (nAChRs) been found uninnervated mammalian cells, immune...
Abstract The maintenance of synaptic functions is essential for neuronal information processing in the adult brain. Astrocytes express glutamate transporters that rapidly remove from extracellular space and they play a critical role precise operation glutamatergic transmission. However, how clearance function astrocytes maintained remains elusive. Here, we describe mechanism uptake capacity Bergmann glial cells (BGs) cerebellum. When inositol 1,4,5‐trisphosphate (IP 3 ) signaling was...
Prostaglandin E2 (PGE2) is well-known as an endogenous proinflammatory prostanoid synthesized from arachidonic acid by the activation of cyclooxygenase-2. E type (EP) receptors are cognates for PGE2 that have four main subtypes: EP1 to EP4. Of these, EP2 and EP4 been shown couple Gαs-protein can activate adenylyl cyclase form cAMP. Studies suggest involved in colorectal homeostasis cancer development, but further work needed identify roles these functions. After sufficient inflammation has...
Poly(ADP-ribosyl)ation is a post-translational modification of proteins by transferring poly(ADP-ribose) (PAR) to acceptor the action polymerase (PARP). Two tankyrase (TNKS) isoforms, TNK1 and TNK2 (TNKS1/2), are ubiquitously expressed in mammalian cells participate diverse cellular functions, including wnt/β-catenin signaling, telomere maintenance, glucose metabolism mitosis regulation. For TNKS1/2 catalyze poly(ADP-ribosyl)ation Axin, key component β-catenin degradation complex, which...
Cytosolic phospholipase A2α (cPLA2α) selectively releases arachidonic acid from membrane phospholipids and has been proposed to be involved in the induction of long-term depression (LTD), a form synaptic plasticity cerebellum. This enzyme requires two events for its full activation: Ca2+-dependent translocation cytosol organelle membranes order access as substrates, phosphorylation by several kinases. However, subcellular distribution activation cPLA2α Purkinje cells role cerebellar LTD have...
Abstract The up‐regulated expression of E‐type prostanoid ( EP ) 4 receptors has been implicated in carcinogenesis; however, the also reported to be weaker tumor tissues than normal tissues. Indeed, have suggested play a role maintenance colorectal homeostasis. This study aimed examine underlying mechanisms/reasons for why inconsistent findings regarding receptor levels homeostasis and carcinogenesis by focusing on cellular densities. Thus, human colon cancer HCA ‐7 cells, which retain some...