A5072053415- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- Biosensors and Analytical Detection
- Respiratory viral infections research
- Advanced biosensing and bioanalysis techniques
- Water Quality Monitoring and Analysis
- Bacteriophages and microbial interactions
- Molecular Biology Techniques and Applications
Dalhousie University
2021-2024
A simple passive sampling protocol using cheesecloth and electronegative filters coupled with a Tween®20-based elution technique provided reliable detection of SARS-CoV-2 in wastewater at targeted locations region low COVID-19 prevalence.
Human viruses pose a significant health risk in freshwater environments, but current monitoring methods are inadequate for detecting viral presence efficiently. We evaluated novel passive in-situ concentration method using granular activated carbon (GAC). This study detected and quantified eight enteric non-enteric, pathogenic recreational lake paired grab GAC samples. The results found that sampling had higher detection rate all compared to samples, with adenovirus be the most prevalent...
The COVID-19 pandemic has underscored the need for effective viral tracking in aqueous environments, particularly non-enteric viruses. Despite advances wastewater monitoring, surveillance of viruses freshwater remains limited due to traditional sampling challenges. This study refines GAC-based passive protocols by determining optimal extraction and elution methods enhancing recovery nucleic acids freshwater. Three commercially available total acid (TNA) kits four buffers were assessed their...
Developing a simple and cost-effective wastewater concentration method using powdered activated charcoal sodium alginate (PAC-NaA) hydrogel beads, enhanced for capturing viruses.
Non-detects—measurements reported as “below the detection limit”—are ubiquitous in environmental science and engineering. They are frequently replaced with a constant, but this biases estimates of means, regression slopes, correlation coefficients. Omitting non-detects is worse, has led to serious errors. Simple alternatives available: rank-based statistics, maximum likelihood estimation, re-purposed survival analysis routines. But many datasets do not align well assumptions these methods...
Abstract Human viruses pose a significant health risk in freshwater environments, but current monitoring methods are inadequate for detecting viral presence efficiently. We evaluated novel passive in-situ concentration method using granular activated carbon (GAC). This study detected and quantified eight enteric non-enteric pathogenic recreational lake paired grab GAC samples. Results found that sampling had higher detection rate all compared to samples, with adenovirus be the most prevalent...