A5012669652- Cellular Mechanics and Interactions
- Cellular transport and secretion
- Cardiomyopathy and Myosin Studies
- Cell Adhesion Molecules Research
- Fungal and yeast genetics research
- Caveolin-1 and cellular processes
- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neuropharmacology Research
- Cell death mechanisms and regulation
- Bacterial Genetics and Biotechnology
- Biotin and Related Studies
- Neurogenesis and neuroplasticity mechanisms
- Genetics and Physical Performance
- Axon Guidance and Neuronal Signaling
University of Sheffield
2016-2024
University of Cambridge
2021-2024
The interaction between cancer cells and the extracellular matrix (ECM) plays a pivotal role in tumour progression. While degradation of ECM proteins has been well characterised, endocytosis its impact on cell progression, migration metastasis is poorly understood. internalisation increased invasive breast cells, suggesting it may support invasiveness. Here we developed high-content screening assay to study uptake. We identified that mitogen-activated protein kinase (MAPK) family members,...
The ubiquitously expressed Na<sup>+</sup>/H<sup>+</sup> exchanger NHE1 plays an important role in regulating polarized membrane protrusion and directional motility non-neuronal cells. Using NGF-differentiated PC12 cells murine neocortical neurons <i>in vitro</i>, we now show that a early neurite morphogenesis. was growth cones which it gave rise to elevated intracellular pH actively extending neurites. inhibitor cariporide reversibly reduced cone filopodia number the formation elongation of...
Actin nucleation is the key rate limiting step in process of actin polymerization, and tight regulation this critical to ensure filaments form only at specific times defined regions cell. Arp2/3 a well-characterised protein complex that can promote new filaments, though its activity requires additional promotion factors (NPFs). The best recognized these are WASP family proteins contain binding motifs for both monomeric Arp2/3. Previously we demonstrated yeast homologue, Las17, addition...
The p21-activated kinase (PAK) family regulate a multitude of cellular processes, including actin cytoskeleton remodelling. Numerous bacterial pathogens usurp host signalling pathways that reorganisation in order to promote Infection. Salmonella and pathogenic Escherichia coli drive actin-dependent forced uptake intimate attachment respectively. We demonstrate the pathogen-driven generation both these distinct structures relies on recruitment activation PAK. show PAK domain is dispensable...
The interaction between cancer cells and the extracellular matrix (ECM) plays a pivotal role in tumour progression. While degradation of ECM proteins has been well characterised, endocytosis its impact on cell progression, migration, metastasis is poorly understood. internalisation increased invasive breast cells, suggesting it may support invasiveness. However, current high-throughput approaches mainly focus grown plastic 2D, making difficult to apply these study dynamics. Here, we...
Abstract P21 activated kinases (PAKs) are involved in a wide range of functions from the regulation cytoskeleton to control apoptosis and proliferation. Although many PAK substrates identified implicated actin cytoskeleton, coherent picture total effect activation on state is unclear. We therefore set out observe quantify inhibition greater detail. In Mouse Embryonic Fibroblasts, kinase activity, either by treatment with small molecule inhibitors or overexpression mutant constructs leads...
Abstract Actin nucleation is the key rate limiting step in process of actin polymerization, and tight regulation this critical to ensure filaments form only at specific times defined regions cell. WH2 domains are short sequence motifs found many different binding proteins including WASP family which regulate nucleating complex Arp2/3. In study we reveal a phosphorylation site, Serine 554, within domain yeast homologue Las17. Both phospho-mimetic mutation reduce monomer affinity while an...