A5044960963- Microtubule and mitosis dynamics
- Cancer Treatment and Pharmacology
- 14-3-3 protein interactions
- Glioma Diagnosis and Treatment
- Ubiquitin and proteasome pathways
- Cancer, Hypoxia, and Metabolism
- Cancer-related Molecular Pathways
- 3D Printing in Biomedical Research
- Nanoplatforms for cancer theranostics
- Cellular Mechanics and Interactions
- Cancer Research and Treatments
- Nanoparticle-Based Drug Delivery
- Mitochondrial Function and Pathology
- Gold and Silver Nanoparticles Synthesis and Applications
- Bioinformatics and Genomic Networks
- Laser-Ablation Synthesis of Nanoparticles
- Cell death mechanisms and regulation
- Cell Image Analysis Techniques
- Angiogenesis and VEGF in Cancer
- Brain Metastases and Treatment
- Cellular transport and secretion
- Cancer Mechanisms and Therapy
- RNA Interference and Gene Delivery
- Neurological disorders and treatments
- ATP Synthase and ATPases Research
Centre National de la Recherche Scientifique
2004-2023
Aix-Marseille Université
2011-2023
Centre de Recherche en Cancérologie de Marseille
2008-2023
Inserm
2008-2023
Institut de Neurophysiopathologie
2018-2023
Hôpital de la Timone
2009-2021
Assistance Publique Hôpitaux de Marseille
2007-2019
Czech Radio
2016
Pharmac
2016
Délégation Provence et Corse
2016
Abstract Capable of generating plasmonic and other effects, gold nanostructures can offer a variety diagnostic therapy functionalities for biomedical applications, but conventional chemically-synthesized Au nanomaterials cannot always match stringent requirements toxicity levels surface conditioning. Laser-synthesized nanoparticles (AuNP) present viable alternative to chemical counterparts exceptional purity (no trace contaminants) unusual chemistry making possible direct conjugation with...
We have previously reported that anti-tubulin agents induce the release of cytochrome c from isolated mitochondria. In this study, we show tubulin is present in mitochondria different human cancerous and non-cancerous cell lines. The absence polymerized microtubules cytosolic proteins was checked to ensure an inherent component addition, a salt wash did not By using electron microscopy, then showed localized mitochondrial membranes. As compared with cellular tubulin, enriched acetylated...
Microtubule dynamics are crucial for mitotic spindle assembly and chromosome movement. Suppression of by Taxol appears responsible the drug's potent ability to inhibit mitosis cell proliferation. Although is an important chemotherapeutic agent, development resistance limits its efficacy. To examine role microtubule in resistance, we measured dynamic instability individual rhodamine-labeled microtubules Taxol-sensitive -resistant living human cancer cells. Taxol-resistant A549-T12 -T24 lines...
This phase II study evaluated the efficacy and safety of a 7-day on/7-day off regimen temozolomide before radiotherapy (RT) in patients with inoperable newly diagnosed glioblastoma.Patients received (150 mg/m2/d on days 1 to 7 15 21 every 28 days; on/7 off) for up four cycles conventional RT (2-Gy fractions total 60 Gy) thereafter or until disease progression. The primary end point was tumor response. Tumor tissue from 25 analyzed O6-methylguanine-DNA methyltransferase (MGMT)...
This paper presents the outcomes from a workshop of European Network on Health and Environmental Impact Nanomaterials (NanoImpactNet). During workshop, 45 experts in field safety assessment engineered nanomaterials addressed need to systematically study sets with specific metrics generate data set which would allow establishment dose-response relations. The group concluded that international cooperation worldwide standardization terminology, reference materials protocols are needed make...
Exhibiting a red-shifted absorption/scattering feature compared to conventional plasmonic metals, titanium nitride nanoparticles (TiN NPs) look as very promising candidates for biomedical applications, but these applications are still underexplored despite the presence of extensive data counterparts. Here, we report fabrication ultrapure, size-tunable TiN NPs by methods femtosecond laser ablation in liquids and their biological testing. We show that demonstrate strong broad peak around...
Abstract Si/SiO x nanoparticles (NPs) produced by laser ablation in deionized water or aqueous biocompatible solutions present a novel extremely promising object for biomedical applications, but the interaction of these NPs with biological systems has not yet been systematically examined. Here, we first comprehensive study biodistribution, biodegradability and toxicity laser-synthesized Si-SiO using small animal model. Despite relatively high dose Si-NPs (20 mg/kg) administered intravenously...
PURPOSE: Most primary oligodendrogliomas and mixed gliomas (oligoastrocytoma) respond to treatment with procarbazine, lomustine, vincristine (PCV), response rates of approximately 80%. However, limited data on second-line treatments are available in patients recurrent tumors. A novel second-generation alkylating agent, temozolomide, has recently demonstrated efficacy safety glioblastoma multiforme anaplastic astrocytoma. This study describes the effects temozolomide oligodendroglioma (AO)...
Abstract Angiogenesis is a critical event in tumor growth and metastasis, which can be inhibited by conventional anticancer drugs such as the microtubule-damaging agent paclitaxel (Taxol). In this study, we investigate mechanism of action on human endothelial cells. We characterize two distinct effects umbilical vein cell microvascular cell-1 proliferation according to drug concentration: cytostatic effect at low concentrations cytotoxic 10 nmol/L. The involves signaling pathways similar...
Abstract BACKGROUND Currently, the survival of patients age > 70 years with glioblastoma multiforme (GBM) ranges from 4 months to 6 months, although radiotherapy and/or chemotherapy may prolong in certain subgroups. Temozolomide is an oral chemotherapeutic agent efficacy against malignant gliomas and a favorable safety profile. This open‐label, single‐center, Phase II study was designed evaluate temozolomide as first‐line exclusive treatment elderly newly diagnosed GBM. METHODS...
Abstract Microtubule-targeted drugs such as paclitaxel exhibit potent antiangiogenic activity at very low concentrations, but the mechanism underlying an effect remains unknown. To understand involvement of microtubules in angiogenesis, we analyzed dynamic instability behavior living endothelial cells [human microvascular (HMEC-1) and human umbilical vascular (HUVEC)] following 4 hours treatment. Unexpectedly, concentrations (0.1-5 nmol/L) strongly increased microtubule overall dynamicity...
We previously reported that paclitaxel acted directly on mitochondria isolated from human neuroblastoma SK‐N‐SH cells. Here, we demonstrate the direct mitochondrial effect of observed in vitro is relevant intact After a 2 h incubation with 1 μM paclitaxel, were less condensed. Paclitaxel (1 μM, 1–4 h) also induced 20% increase respiration rate and caspase‐independent production reactive oxygen species by mitochondria. The paclitaxel‐induced release cytochrome c was detected only after 24...
Actin assembly at the cell front drives membrane protrusion and initiates migration cycle. Microtubules (MTs) extend within forward protrusions to sustain polarity promote adhesion site turnover. Memo is an effector of ErbB2 receptor tyrosine kinase involved in breast carcinoma migration. However, its mechanism action remained unknown. We report this study that controls ErbB2-regulated MT dynamics by altering transition frequency between growth shortening phases. Moreover, although...
We employ a method of femtosecond laser fragmentation preliminarily prepared water-dispersed microcolloids to fabricate aqueous solutions ultrapure bare Si-based nanoparticles (Si-NPs) and assess their potential for biomedical applications. The appear spherical in shape, with low size dispersion controllable mean size, from few nm several tens nm, while negative surface charge (-35 mV ± 0.10 according z-potential data) provides good electrostatic stabilization colloidal Si-NP solutions....
Abstract Discodermolide is a new microtubule-targeted antimitotic drug in Phase I clinical trials that, like paclitaxel, stabilizes microtubule dynamics and enhances polymer mass vitro cells. Despite their apparently similar binding sites on microtubules, discodermolide acts synergistically with paclitaxel to inhibit proliferation of A549 human lung cancer cells (L. Martello et al., Clin. Cancer Res., 6: 1978–1987, 2000). To understand synergy, we examined the effects two drugs singly...
Human immunodeficiency virus (HIV) infection and the progression to AIDS are characterized by depletion of CD4(+) T-cells. HIV-1 leads apoptosis uninfected bystander cells direct killing HIV-infected cells. This is mediated, in part, Tat protein, which secreted virally infected taken up We chemically synthesized two 86-residue subtype D proteins, Ug05RP Ug11LTS, from Ugandan patients who were clinically categorized as either rapid progressor or long-term survivor, with non-conservative...
Integrins are essential protagonists of the complex multi-step process angiogenesis that has now become a major target for development anticancer therapies. We recently reported and characterized MVL-PLA2, novel phospholipase A2 from Macrovipera lebetina venom, exhibited anti-integrin activity. In this study, we show MVL-PLA2 also displays potent anti-angiogenic properties. This inhibited adhesion migration human microvascular-endothelial cells (HMEC-1) in dose-dependent manner without being...
Metastasis, a fatal complication of breast cancer, does not fully benefit from available therapies. In this study, we investigated whether ATIP3, the major product 8p22 MTUS1 gene, may be novel biomarker and therapeutic target for metastatic tumors. We show that ATIP3 is prognostic marker overall survival among patients with cancer. Notably, tumors, low levels associate decreased patients. By using well-defined experimental mouse model cancer metastasis, expression delays time-course...
Phosphorylated tau protein is the major component of paired helical filaments in Alzheimer disease (AD). We have previously shown that abnormal phosphorylation was induced neuroblastoma SK-N-SH cells by anticancer drug, paclitaxel, during apoptosis [Guise et al., 1999: Apoptosis 4:47-58]. In present study, we first demonstrated a shift from fetal to hyperphosphorylated after incubation with showed some similarities AD, using several antibodies, N-Term, Tau-1 and AT-8. Tau occurred...