A5018782365- Bacteriophages and microbial interactions
- Cancer Cells and Metastasis
- Microbial Community Ecology and Physiology
- Wnt/β-catenin signaling in development and cancer
- RNA Research and Splicing
- Antibiotic Resistance in Bacteria
- Epigenetics and DNA Methylation
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- CRISPR and Genetic Engineering
- Congenital Diaphragmatic Hernia Studies
- Retinal and Optic Conditions
- Trace Elements in Health
- Cancer-related Molecular Pathways
- Protist diversity and phylogeny
- Genetic Mapping and Diversity in Plants and Animals
- Animal Genetics and Reproduction
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer-related gene regulation
- Biosensors and Analytical Detection
- Heat shock proteins research
- Sperm and Testicular Function
- DNA Repair Mechanisms
- Drug Transport and Resistance Mechanisms
- Heavy Metal Exposure and Toxicity
Duke University
2024
Princeton University
2005-2021
Rutgers, The State University of New Jersey
2001
The stem cell niche is a specialized environment that dictates function during development and homeostasis. We show Dll1, Notch pathway ligand, enriched in mammary gland cells (MaSCs) mediates critical interactions with stromal macrophages the surrounding mouse models. Conditional deletion of Dll1 reduced number MaSCs impaired ductal morphogenesis gland. Moreover, MaSC-expressed activates signaling macrophages, increasing their expression Wnt family ligands such as Wnt3, Wnt10A, Wnt16,...
The transcription factor E74-like 5 (Elf5) functions downstream of the prolactin receptor signaling pathway and plays an important role in mammary gland development. Using conditional mouse knockouts, we have previously shown that Elf5-null glands exhibit a complete failure alveologenesis during pregnancy. developmental phenotype is mediated through alteration expression several critical genes involved alveologenesis, particularly those belonging to JAK/STAT pathway. Here, demonstrate...
Bone marrow is both a primary site for blood cell production and fertile niche cancer metastasis. The mechanism of common occurrence anemia among patients with bone metastasis remains poorly understood. Here, we identified specialized population VCAM1+CD163+CCR3+ macrophages, which are typically essential erythropoiesis, become highly enriched in metastatic support tumor growth. Hijacking these macrophages reduces iron availability erythroblasts, impairing erythropoiesis contributing to...
Potential mechanisms underlying impaired chemotactic responsiveness of neonatal neutrophils were investigated. Two distinct chemoattractants compared: bacterially derived N-formyl-methionyl-leucyl-phenylalanine (fMLP) and a unique monoclonal antibody, designated DL1.2, which binds to neutrophil antigen with an apparent molecular mass 120 kDa. Chemotaxis toward fMLP, as well was reduced in neonates when compared adult cells. This did not appear be result decreased fMLP receptor or DL1.2...
Despite being a major component of the pseudorabies virus tegument, VP22 is not required for PRV replication, virulence, or neuroinvasion (T. del Rio, H. C. Werner, and L. W. Enquist, J. Virol. 76:774-782, 2002). In absence VP22, tegument assembly compensates in limited fashion with increased incorporation cellular actin. Infection epithelial cell lines expressing fluorescent actin fusion proteins resulted filamentous nonfilamentous into individual virions that were predominately light,...
Mammary stem cells (MaSCs) play essential roles for the development of mammary gland and its remodeling during pregnancy. However, precise localization MaSCs in their regulation pregnancy is unknown. Here we report a transgenic mouse model luciferase-based single marker detection vivo that used to address these issues. Single transgene expressing epithelial were shown reconstitute glands while immunohistochemical staining identified basal luminal locations, with preponderance towards...
Keystone species or ecological engineers are vital to the health of an ecosystem; however, often, their low abundance biomass present challenges for discovery, identification, visualization and selection. We report development fluorescent in situ hybridization transcript-annealing molecular beacons (FISH-TAMB), a fixation-free protocol that is applicable archaea bacteria. The FISH-TAMB method differs from existing FISH methods by absence fixatives surfactants buffers, fast time as short 15...
Although the haplotype structure of human genome has been studied in great detail, very little is known about mechanisms underlying its formation. To investigate role meiotic recombination on block formation, single nucleotide polymorphisms were selected at a high density from 2.5-Mb region chromosome 21. Direct analysis by high-throughput multiplex genotyping 662 sperm identifies 41 recombinants. The crossovers nonrandomly distributed within 16 small areas. All, except one, these fall areas...
Abstract Metallothioneins (MT) are low-molecular-weight, cysteine-rich proteins that induced in response to a variety of chemical stresses and therefore can be used assess human exposure environmental agents. In the current study, flow cytometry was characterize basal cadmium-induced expression MT three major leukocyte populations peripheral blood. analysis, monocytes were most sensitive leukocytes this toxic metal, with significant increases cellular levels being detected at concentrations...
Chronic anterior uveitis (CAU) carries a significant risk for eye complications and vision loss. The Childhood Arthritis Rheumatology Research Alliance (CARRA) introduced consensus treatment plans (CTPs) to standardize CAU facilitate future comparative effectiveness studies. Two CTPs were developed address: 1) initiation of methotrexate (MTX) in patients with naïve steroid-sparing therapy, 2) TNF inhibitor (TNFi) severe or refractory MTX. We evaluated implementation the using existing CARRA...
ABSTRACT High-throughput sequencing and cellular imaging have expanded our knowledge of microbial diversity expression activity. However, it remains challenging to characterize low-abundance, slow-growing microorganisms that play key roles in biogeochemical cycling. With the goal isolating transcriptionally active cells these from environmental samples, we developed fluorescent situ hybridization transcript-annealing molecular beacons (FISH-TAMB) label living prokaryotic cells. FISH-TAMB...
Abstract Keystone species or ecological engineers are vital to the health of an ecosystem, however, often their low abundance biomass present challenges for discovery, identification, visualization and selection. We report development fluorescent in situ hybridization transcript-annealing molecular beacons (FISH-TAMB), a fixation-free protocol that is applicable archaea bacteria. The FISH-TAMB method differs from existing FISH methods by absence fixatives surfactants buffers, fast time as...