A5008556740- Microfluidic and Capillary Electrophoresis Applications
- Biosensors and Analytical Detection
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Bio-sensing Technologies
- Electrowetting and Microfluidic Technologies
- Analytical Chemistry and Sensors
- Molecular Biology Techniques and Applications
- Environmental DNA in Biodiversity Studies
- Analytical Chemistry and Chromatography
- Epigenetics and DNA Methylation
- Advanced Data Storage Technologies
University of Virginia
2020-2024
The polymerase chain reaction (PCR) is paramount in nucleic acid amplification testing, and for many assays, the use of PCR or qPCR considered 'gold standard'. While instrumentation executing has advanced over last two decades, a growing interest point-of-need testing highlighted deficit that exists 'rapid PCR' systems. Here, we describe field-forward prototype instrument capable ultra-fast thermal cycling real-time DNA RNA. custom-designed, injection-molded microfluidic chips interface with...
ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTMicrochip Electrophoresis for Fluorescence-Based Measurement of Polynucleic Acids: Recent DevelopmentsRenna L. Nouwairi*Renna NouwairiDepartment Chemistry, University Virginia, Charlottesville, Virginia 22903, United States*Email: [email protected]More by Renna NouwairiView Biographyhttp://orcid.org/0000-0003-4013-3126, Killian C. O'ConnellKillian O'ConnellDepartment StatesMore O'ConnellView Biography, Leah M. GunnoeLeah GunnoeDepartment GunnoeView...
Presumptive drug screening enables timely procurement of search and arrest warrants represents a crucial first step in crime scene analysis. Screening also reduces the burden on forensic laboratories which often face insurmountable backlogs. In most scenarios, on-site presumptive relies chemical field tests for initial identification. However, even when used appropriately, these test kits remain limited to subjective colorimetric analysis, produce false positive or negative results with...
An alternative method for epigenetic sample preparation by a rotationlly-driven, microfluidic sodium bisulfite conversion system up to four samples in parallel.
The laser print, cut, and laminate (PCL) method for microfluidic device fabrication can be leveraged rapid inexpensive prototyping of electrophoretic microchips useful optimizing separation conditions. capability allows the evaluation fluidic architecture, applied fields, reagent concentrations, sieving matrix, all within context using fluorescence-compatible substrates. Cyclic olefin copolymer toner-coated polyethylene terephthalate (tPeT) were utilized with PCL technique bonding methods...
Optimizing multireagent assays often requires successive titration of individual components until the optimal combination conditions is achieved. This process time-consuming, laborious, and expensive since parallelized experimentation bulk consumption reagents. Microfluidics presents a solution through miniaturization standard processes by reducing reaction volume, executing multiple parallel workflows, enabling automation. While single-digit microliter reactions can be effective, scaling to...
Initial screening of criminal evidence often involves serological testing stains unknown composition and/or origin discovered at a crime scene to determine the tissue origin. This is presumptive but critical for contextualizing scene. Here, we describe microfluidic approach body fluid profiling via fluorescent electrophoretic separation published mRNA panel that provides unparalleled specificity and sensitivity. centrifugal expedites automates electrophoresis process by allowing simple,...