A5030527876- Viral Infectious Diseases and Gene Expression in Insects
- Protein purification and stability
- Fungal and yeast genetics research
- Biosimilars and Bioanalytical Methods
- Mineral Processing and Grinding
- Fungal Biology and Applications
- Microfluidic and Bio-sensing Technologies
- Microbial Metabolic Engineering and Bioproduction
- Biomedical and Engineering Education
- Transgenic Plants and Applications
AstraZeneca (Brazil)
2023
AstraZeneca (United States)
2021
Eli Lilly (United States)
2010
Chinese hamster ovary (CHO) cell cultures are commonly used for production of recombinant human therapeutic proteins. Often the goal such a process is to separate growth phase cells, from non-growth where ideally cells diverting resources produce protein interest. Characterizing way that use nutrients in terms metabolic fluxes as function culture conditions can provide deeper understanding biology offering guidance improvements. To evaluate fluxes, flux analysis CHO was performed by...
Abstract An 8 ton per year manufacturing facility is described based on the framework for integrated and continuous bioprocessing (ICB) common to all known biopharmaceutical implementations. While output of this plant rivals some largest fed‐batch plants in world, equipment inside relatively small: consists four 2000 L single‐use bioreactors has a maximum flow rate 13 L/min. The ICB sufficient detail allow companies, vendors, contract manufacturers build or buy their own systems. design will...
In the production of biopharmaceuticals disk-stack centrifugation is widely used as a harvest step for removal cells and cellular debris. Depth filters followed by sterile are often then employed to remove residual solids remaining in centrate. Process development usually conducted at pilot-scale so mimic commercial scale equipment but this method requires large quantities cell culture significant levels effort successful characterization. A scale-down approach based upon use shear device...
In the production of biopharmaceuticals depth filters followed by sterile are often employed to remove residual cell debris present in feed stream. back drop a global pandemic, supply chains associated with have been constrained. These constraints limited available amount for manufacture biologics. This has placed manufacturing facilities difficult position having choose between running processes reduced number and risking failed batch or prospect plants going into temporary shutdown until...
As discussed in the previous chapters of this book, mammalian cell-culture technology facilitates production complex therapeutic proteins (biopharmaceutical drugs). The process is performed with a host cell line that has been transfected DNA plasmids encoding for protein interest. selected based on post-translational modifications necessary efficacy. most common host-cell lines reported are Chinese hamster ovary (CHO) or murine myeloma (SP2/0, NSO). site and number inserted into during...