A5060160521- Fish Ecology and Management Studies
- Pluripotent Stem Cells Research
- Retinal Diseases and Treatments
- Aquatic Invertebrate Ecology and Behavior
- Retinal Imaging and Analysis
- 3D Printing in Biomedical Research
- Fish Biology and Ecology Studies
- Aquatic Ecosystems and Phytoplankton Dynamics
- Marine and fisheries research
- Tissue Engineering and Regenerative Medicine
- Isotope Analysis in Ecology
- Pancreatic function and diabetes
- Species Distribution and Climate Change
- Glaucoma and retinal disorders
- Aquaculture Nutrition and Growth
- Animal and Plant Science Education
- Mesenchymal stem cell research
- Renal and related cancers
- Angiogenesis and VEGF in Cancer
- Fish biology, ecology, and behavior
- Cerebral Venous Sinus Thrombosis
- Mathematical Biology Tumor Growth
- Soil and Water Nutrient Dynamics
- Soil Moisture and Remote Sensing
- Water Quality and Resources Studies
University of Southampton
2013-2022
University of Winchester
2019
University Hospital Southampton NHS Foundation Trust
2017
Westfield State University
2009-2015
Centre For Human Genetics
2014
Applied Research Associates (United States)
2014
University of Oxford
2011
Alaska Biological Research (United States)
2011
Teck (Canada)
2011
Washington State University
2007-2009
Energy metabolism is intrinsic to cell viability but surprisingly has been little studied in human embryonic stem cells (hESCs). The current study aims investigate the effect of environmental O2 tension on carbohydrate utilisation hESCs. Highly pluripotent hESCs cultured at 5% consumed significantly more glucose, less pyruvate and produced lactate compared those maintained 20% O2. Moreover, atmospheric levels expressed OCT4, SOX2 NANOG than To determine whether this difference was a...
SUMMARY Candida albicans systemic dissemination in immunocompromised patients is thought to develop from initial gastrointestinal (GI) colonisation. It unclear what components of the innate immune system are necessary for preventing C. GI tract, but studies mice have indicated that both neutropenia and mucosal damage crucial allowing widespread invasive disease. Mouse models, however, provide limited applicability genome-wide screens pathogen or host factors – might influence following For...
Low O2 tension is beneficial for human embryonic stem cell (hESC) maintenance but the mechanism of regulation unknown. HIF-2α was found to bind directly predicted hypoxic response elements (HREs) in proximal promoter OCT4, NANOG and SOX2 only hESCs cultured under hypoxia (5% O2). This binding induced an array histone modifications associated with gene transcription while a heterochromatic state existed at atmospheric O2. Interestingly, enhanced euchromatic when were exposed followed by 72...
Abstract Human embryonic stem cells (hESCs) have the capacity to differentiate into all cell types and thus great potential for regenerative medicine. hESCs cultured at low oxygen tensions are more pluripotent display an increased glycolytic rate but how this is regulated unknown. This study therefore aimed investigate regulation of glucose metabolism in whether might impact OCT4 expression. In contrast transporter GLUT1, GLUT3 was by environmental localised hESC membranes. Silencing caused...
Abstract STUDY QUESTION Does the type of incubator used to culture human preimplantation embryos affect development blastocyst stage and alter amino acid utilization in assisted reproduction? SUMMARY ANSWER Culturing a time lapse system (TLS) was associated with higher Day 5 formation rate altered when measured from 3 compared standard benchtop incubator. WHAT IS KNOWN ALREADY Culture environment is known be important for developing embryo. TLSs provide stable milieu allowing monitored situ,...
Abstract Background A dynamic vasculature is a prerequisite for bone formation where the interaction of cells and endothelial essential both development healing process bone. Enhanced understanding specific mediators involved in cell interactions offers new avenues skeletal regenerative applications. This study has investigated osteogenic angiogenic potential co-cultures human foetal diaphyseal or epiphyseal with umbilical vein (HUVEC) presence absence vascular growth factor (VEGF)...
Abstract Christensen, D.R. and B.C. Moore. 2009. Using stable isotopes a multiple-source mixing model to evaluate fish dietary niches in mesotrophic lake. Lake Reserv. Manage. 25:167–175. We used isotope analysis (SIA) of δ13C δ15N within the Twin Lakes, Washington, community identify potential for exploitative competition, resource partitioning, predation. The SIA revealed distinct spatial feeding niches; pelagic species were depleted, while littoral organisms enriched. Trophic identified...
Background/Aims: Human embryonic stem cells (hESCs) are a potential source of for treatment many degenerative diseases, but in culture have propensity to spontaneously differentiate, possibly due suboptimal conditions. Culture at low oxygen tensions improves hESC maintenance and regulates carbohydrate metabolism. Hence, greater understanding the nutrient requirements hESCs will allow production more appropriate media. This study aims investigate effect environmental tension on amino acid...
Microfloc alum injection, in contrast to whole-lake treatments, is a relatively new technique for delivery that has been implemented small number of lakes and reservoirs. injection primarily designed precipitate phosphorus the water column using low doses applied on continuous or intermittent basis. The employs diffusers create ultra-small aluminum hydroxide particles with lower settling velocities longer residence times, compared whole lake strip dissolved from column. Because few microfloc...
Sorsby fundus dystrophy (SFD) is a rare autosomal dominant disease of the macula that leads to bilateral loss central vision and caused by mutations in TIMP3 gene. However, mechanisms which cause SFD are poorly understood. Here, we generated human induced pluripotent stem cell-derived retinal pigmented epithelial (hiPSC-RPE) cells from three patients carrying p.(Ser204Cys) non-affected controls study disease-related structural functional differences RPE. SFD-hiPSC-RPE exhibited...
ABSTRACT In Twin Lakes, Washington, illegal introductions of largemouth bass (Micropterus salmoides) and golden shiner (Notemigonus crysoleucas) are feared to be impacting economically important rainbow trout (Oncorhynchus mykiss) brook (Salvelinus fontinalis) populations. We evaluated the stomach contents 69 shiner, 146 trout, 83 561 during summer stratification in 2004 2005, determine community diet composition overlap when food resources were partitioned thermally spatially. Gut content...
Abstract Largemouth bass (Micropterus salmoides) can be top-down regulators in a fish community. It is important for fisheries biologists who manage predator-prey populations to understand when become piscivorous. We examined the stomach contents of 622 largemouth watershield (Brasenia schreberi) beds North and South Twin Lakes, Washington. Bass displayed temporal ontogenetic diet variation within between lakes. <100 mm fed principally on zooplankton scuds June September benthic...
Summer temperature/dissolved oxygen squeeze conditions have negatively impacted coldwater fisheries, including rainbow (Oncorhynchus mykiss) and brook trout (Salvelinus fontinalis), in North South Twin lakes of northeastern Washington. In 2009, line diffuser hypolimnetic oxygenation (HO) was initiated to prevent anoxia increase summer habitat. Suitable habitat volume increased from a low 0% preoxygenation 100% the hypolimnion following HO. To assess if utilized expanded oxygenation,...
High mortality (84–89%) of stocked rainbow trout (Oncorhynchus mykiss) in Twin Lakes, Washington, has been partially blamed on predation from non-native largemouth bass (Micropterus salmoides). We examined the gut-content 434 and applied a bioenergetics model to determine consumption demand hatchery-released other prey species Lakes. Largemouth consumed approximately 4915 (95% CI; 2393–13,452) fall South Twin. No was observed North Twin, despite similar stocking regime. Approximately 6.3%...
Species-specific habitat suitability models have potential for use in restoration efforts, but their efficiency still remains question. As eastern brook trout, Salvelinus fontinalis, populations New England declined over the last few decades as a result of anthropogenic and natural impacts on critical habitat, become common tool conservation efforts. These models, however, inherent flaws that prevent widespread uniform use. To better adapt these must be properly addressed. Using modified...
Located in the Western Brooks Range of Alaska, Red Dog Mine is one most northerly active, zinclead open pit mines.Mining high grade zinc and lead sulphide ores results production waste rock that mostly acid generating.Control treatment drainage (ARD) from walls critical environmental activities at mine site.The ARD water main stockpile, containing total dissolved solids (TDS) values as 90,000 mg/L, expensive today it will be a major component post-closure costs future.In order to reduce...
248 Background: 90 Y-microsphere therapy (radioembolization) is an effective and safe brachytherapy procedure for control of unresectable primary metastatic hepatic malignancies. To advance this treatment approach, a method proposed which enables complex modeling the arterial route, tumor microvascular bed in radioactive particles will become permanently embedded. Methods: Prior work from our group included vessel measurements patients undergoing including geometry, pressure, velocity...