A5049880729- Colorectal Cancer Surgical Treatments
- Gastric Cancer Management and Outcomes
- Colorectal and Anal Carcinomas
- Gastrointestinal Tumor Research and Treatment
- Chemokine receptors and signaling
- Colorectal Cancer Screening and Detection
- Cancer Cells and Metastasis
- Cytokine Signaling Pathways and Interactions
- Metastasis and carcinoma case studies
- Colorectal Cancer Treatments and Studies
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cardiac, Anesthesia and Surgical Outcomes
- Immunotherapy and Immune Responses
- Anorectal Disease Treatments and Outcomes
- Ferroptosis and cancer prognosis
- Peptidase Inhibition and Analysis
- Genetic factors in colorectal cancer
- Diverticular Disease and Complications
- Intraperitoneal and Appendiceal Malignancies
- Pancreatic and Hepatic Oncology Research
- Angiogenesis and VEGF in Cancer
- Appendicitis Diagnosis and Management
- Minimally Invasive Surgical Techniques
- Wnt/β-catenin signaling in development and cancer
- Cancer Genomics and Diagnostics
Ruijin Hospital
2015-2024
Shanghai Jiao Tong University
2013-2023
Shanghai Institute of Hematology
2009-2017
Shanghai East Hospital
2011-2016
Tongji University
2012-2015
Shanghai Police College
2011
The University of Sydney
2009
Center for Special Minimally Invasive and Robotic Surgery
2006-2008
Duke University Hospital
2008
Duke Medical Center
2008
Metastasis is a major cause of death in human colorectal cancer patients. However, the contribution chemokines tumor microenvironment to metastasis not fully understood.Herein, we examinined several patients using chemokine ELISA array. Immunohistochemistry was used detect expression CXCL5 tissues. Human HCT116 and SW480 cell lines stably transfected with CXCL5, shCXCL5 shCXCR2 lentivirus plasmids were our vitro study. Immunoblot, immunofluorescence transwell assay examine molecular biology...
The mechanisms underlying the role of CXCL5 in tumor angiogenesis have not been fully defined. Here, we examined effect on colorectal cancer (CRC). Immunohistochemistry was used to monitor expression and CD31 CRC patients' tissues. HUVEC cell lines stably transfected with shCXCR2 shFOXD1 lentivirus plasmids were an vitro study. Based some molecular biological experiments vivo, found that upregulated tissues its level positively correlated CD31. Next, recombinant human (rhCXCL5) stimulate...
Abstract The mechanisms underlying the role of chemokines in tumor angiogenesis is still not fully understood. In this study, we detected influence CCL19 on colorectal cancer (CRC) angiogenesis. expression and CD31 CRC tissues were by immunohistochemistry. Human cell lines SW1116 SW620 stably transfected with lentivirus shRNA, HUVEC CCR7 shRNA used our study. Our study showed that was significantly low-expressed positively related to highly microvessel density. vitro, observed high-expressed...
N-myc downstream-regulated gene 1 (NDRG1) has been reported to act as a key regulatory molecule in tumor progression-related signaling pathways, especially metastasis. However, the related mechanism not fully discovered yet. Herein we demonstrated that novel of cell migration and invasion, caveolin-1, direct interaction with NDRG1 human colorectal cancer (CRC) cells. Moreover, reduces caveolin-1 protein expression through promoting its ubiquitylation subsequent degradation via proteasome CRC...
Abstract Background Interleukin-17A (IL-17A), a proinflammatory cytokine primarily secreted by Th17 cells, γδT cells and natural killer T (NKT) performs essential roles in the microenvironment of certain inflammation-related tumours regulating cancer growth tumour elimination proved previous literature. In this study, mechanism IL-17A that induces mitochondrial dysfunction promoted pyroptosis has been explored colorectal cells. Method The records 78 patients diagnosed with CRC were reviewed...
Abstract Oxaliplatin resistance is a major challenge in the treatment of colorectal cancer (CRC). Many molecular targeted drugs for refractory CRC have been developed to solve drug resistance, but their effectiveness and roles progression oxaliplatin remain unclear. Here, we successfully constructed PDOs selected Kruppel-like factor 5 (KLF5) inhibitor ML264 as research object based on results vitro screening assay. significantly restored sensitivity by restoring apoptotic response, this...
Background:Polo-like kinase 1 (PLK1) is an important molecule in proliferation of many human cancers. The aim study to clarify the expression patterns and potential function PLK1 colorectal cancers.Material/Methods:Fifty-six cancers samples were collected arranged onto a tissue array detected by immunohistochemistry correlated with clinico-pathological characteristics PCNA. Expression 9 cancer cells lines was investigated RT-PCR Western blot, then SW1116 treated siRNA efficiency examined...
Chemokines and chemokine receptors play an important role in tumorigenesis. Angiogenesis is a vital part of the occurrence, development metastasis cancer. CCR6 factor during tumor progression; however, its function angiogenesis not fully understood. In our study, we found that was significantly overexpressed colorectal cancer (CRC) tissues predicted poor prognosis CRC patients. We then verified on vivo vitro. observed silencing could decrease by inhibiting proliferation migration human...
CXCL5 is overexpressed in colorectal cancer (CRC) and promotes distant metastasis angiogenesis of tumors; however, the underlying mechanism that mediates overexpression CRC remains unclear. Here, we successfully extracted identified primary mesenchymal stromal cells (MSCs) verified promoting effects tumor-associated MSCs on proliferation vivo vitro. We found not only promoted expression by secreting CCL7 but also secreted TGF-β to inhibit this process. After secretion, CCL7/CCR1 activated...
To investigate the roles of ribonucleotide reductase M2 (RRM2) subunit in colorectal cancer (CRC) and ultraviolet (UV)-induced DNA damage repair.Immunohistochemical staining tissue microarray was performed to detect expression RRM2. Seven CRC cell lines were cultured three human colon lines, i.e., HCT116, SW480 SW620, used. Reverse transcription polymerase chain reaction Western blotting determine mRNA protein levels RRM2, respectively. Cell proliferation assay, cycle analysis performed....
To discuss the learning curve of laparoscopy-assisted distal gastrectomy (LADG) with lymph node dissection and oncological results long-term outcomes different periods in stage.One hundred twenty-four patients gastric cancer who received LADG from January 2004 to December 2009 were retrospectively reviewed analyzed. They divided into three groups (A-C) according operative date: 41 Group A (early), B (middle), 42 C (laer). There no significant statistical differences among respect age,...
// Xiongzhi Wangpu 1, 2, 3 , Xiao Yang 4 Jingkun Zhao 2 Jiaoyang Lu Shaopei Guan Jun Zaklina Kovacevic Wensheng Liu 1 Lan Mi Runsen Jin Jing Sun Fei Yue Junjun Ma Aiguo Des R. Richardson Lishun Wang 5 Minhua Zheng Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School Medicine, Shanghai, 200025, China Minimally Invasive Surgery Center, Molecular Pharmacology and Pathology Program, Bosch Institute, Sydney, New South Wales, 2006, Australia Institute Digestive The...
Cadherin-12 (CDH12) is a subtype of N-cadherin family. In this study, we investigated the expression CDH12 and role in prognosis colorectal cancer (CRC) patients. addition, observed influence on proliferation progression CRC cell lines. By using immunohistochemical staining, analyzed samples adjacent non-tumor tissues collected from 78 patients who underwent laparoscopic surgery Shanghai Minimally Invasive Center, China. Statistical analyses were used to analyze relationship between tumor...
Chemokines and chemokine receptors are causally involved in the metastasis of human malignancies. As a crucial receptor for mediating immune homeostasis, however, role CCR4 colorectal cancer (CRC) remains unknown. In this study, we found that high expression CRC tissues was correlated with shorter overall survival disease free survival. vitro vivo experiments revealed silencing attenuated invasion cells, whereas ectopic overexpression contributed to forced these cells. We further...
Abstract Background: Up to now, there is still no ideal tumor marker in early diagnosis and effective monitoring, especially for surgical resection of colorectal cancer (CRC). The aim the present study was evaluate application urinary normal modified nucleosides surgery monitoring CRC. Methods: Between October 2002 July 2003, 52 consecutive patients with pathological confirmed CRC were enrolled. Spontaneous urine samples collected 1 day before on 8 postoperatively, 14 determined by...
Radiotherapy remains one of the cornerstones to improve outcome colorectal cancer (CRC) patients. CRC not only help destroy cells but also remodel tumour microenvironment by enhancing tumour-specific tropism bone marrow-derived mesenchymal stromal cell (BM-MSC) from peripheral circulation. However, role local MSCs and recruited BM-MSC under radiation were well defined. Indeed, functions without irradiation intervention remained controversial in progression: was previously shown modulate...
Microsatellite instability (MSI) has emerged as one of the key biological features colorectal cancer (CRC). However, controversies remain regarding association between MSI status and clinicopathological characteristics CRC. Therefore, it is crucial to identify potential genes pathways associated with in In present study, GSE25071 gene expression profile was retrieved, thirty‑eight cases microsatellite stable (MSS), five MSI‑High (MSI‑H) three MSI‑Low (MSI‑L) CRC patients. The differentially...