A5056039245- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Force Microscopy Techniques and Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Circular RNAs in diseases
- Biotin and Related Studies
- HIV/AIDS drug development and treatment
- Erythrocyte Function and Pathophysiology
- Neurogenesis and neuroplasticity mechanisms
- Surface and Thin Film Phenomena
- Glycosylation and Glycoproteins Research
- Cancer therapeutics and mechanisms
- Developmental Biology and Gene Regulation
- Alzheimer's disease research and treatments
- Antimicrobial Peptides and Activities
- Biochemical effects in animals
- Acoustic Wave Resonator Technologies
- Polyomavirus and related diseases
- RNA and protein synthesis mechanisms
- Biomarkers in Disease Mechanisms
- Optical Coatings and Gratings
- Genetics and Neurodevelopmental Disorders
- Coenzyme Q10 studies and effects
Indian Institute of Science Education and Research Kolkata
2025
Scripps Research Institute
2010-2022
Powertech Technology (Taiwan)
2013
National Centre for Biological Sciences
2002-2008
Tata Institute of Fundamental Research
2008
Cold Spring Harbor Laboratory
2006
Beth Israel Deaconess Medical Center
1997
Harvard University
1997
Significance Exosomes have been implicated in intercellular communication cancer and neurodegenerative disorders. We explored their function brain development. Proteomic analysis demonstrated that exosomes from isogenic control cultures contain neurodevelopmental signaling proteins, which are lacking MECP2 loss-of-function (MECP2LOF) cultures. Treating MECP2LOF neural with rescues deficits, increasing neurogenesis, synaptogenesis, network activity. similarly vivo: injecting purified into the...
Mass spectrometric strategies to identify protein subpopulations involved in specific biological functions rely on covalently tagging biotin proteins using various chemical modification methods. The tag is primarily used for enrichment of the targeted subpopulation subsequent mass spectrometry (MS) analysis. A limitation these that MS analysis does not easily discriminate unlabeled contaminants from labeled under study. To solve this problem, we developed a flexible method only relies direct...
The Leu-74 to Val (Leu74Val) mutation in human immunodeficiency virus type 1 reverse transcriptase (RT) develops as a consequence of didanosine (ddI) therapy and is associated with decreased susceptibility ddI. In this report, we provide evidence that the ddI-associated Leu74Val confers replication disadvantage virus. series experiments, have shown (i) cloned an engineered RT was attenuated for replication; (ii) Val-to-Leu revertant pNL4-3 background replicated efficiency similar wild-type...
Abstract Polymeric surfactants, valued for their ability to stabilize interfaces and tunable self‐assembled structures, find extensive applications in personal care, drug delivery, pharmaceuticals, industrial formulations. To develop an efficient polymeric surfactant, herein we investigate the synthesis characterization of side‐chain poly(ethylene glycol) ( PEG )‐based homopolymers PPEGMAx ), using reversible addition‐fragmentation chain transfer (RAFT) polymerization presence a hydrophobic...
Intercellular transfer of toxic proteins between neurons is thought to contribute neurodegenerative disease, but whether direct interneuronal protein occurs in the healthy brain not clear. To assess prevalence and identity transferred cellular specificity transfer, we biotinylated retinal ganglion cell vivo examined transported through rodent visual circuit using microscopy, biochemistry, mass spectrometry. Electron microscopy demonstrated preferential from retinogeniculate inputs excitatory...
Abstract Nonmammalian vertebrates have a remarkable capacity to regenerate brain tissue in response central nervous system (CNS) injury. Nevertheless, it is not clear whether animals recover lost function after injury or injury‐induced cell proliferation mediates recovery. We address these questions using the visual and visually‐guided behavior Xenopus laevis tadpoles. established reproducible means produce unilateral focal optic tectal neurons without damaging retinotectal axons. then...
Neuronal activity initiates signaling cascades that culminate in diverse outcomes including structural and functional neuronal plasticity, metabolic changes. While studies have revealed activity-dependent cell type-specific transcriptional changes, unbiased quantitative analysis of cell-specific activity-induced dynamics newly synthesized proteins (NSPs) synthesis vivo has been complicated by cellular heterogeneity a relatively low abundance NSPs within the proteome brain. Here we combined...
The brain processes information and generates cognitive motor outputs through functions of spatially organized proteins in different types neurons. More complete knowledge their distributions within neuronal compartments intact circuits would help the understanding function. We used unbiased vivo protein labeling with intravitreal NHS-biotin for discovery analysis endogenous axonally transported visual system using tandem mass spectrometric proteomics, biochemistry, both light electron...
Topotecan (TPT), a known inhibitor of topoisomerase I, has previously been shown to inhibit the replication several viruses. The mechanism inhibition was proposed be I. We report that TPT decreased human immunodeficiency virus type 1 (HIV-1) in CPT-K5, cell line with I mutation. inhibited production HIV-1 RNA and p24 CPT-K5 wild-type cells equally effectively. antiviral effects were observed not only topoisomerase-mutated but also peripheral blood mononuclear (PBMC) acutely infected clinical...
Summary Exosomes are thought to be secreted by all cells in the body and involved intercellular communication. Here, we tested whether neural exosomes regulate development of circuits exosome-mediated signaling may aberrant neurodevelopmental disorder Rett Syndrome (RTT). Quantitative proteomic analysis comparing from human induced pluripotent stem (hiPSC) - derived RTT patient or control cultures indicates that contain components capable influencing neuronal function, which lacking...
Determination of morphology and size distribution primary secondary abrasives slurry is essential for optimization CMP performance. The undiluted was characterized in liquid phase using silicon based Nano-pipettes TEM. To validate utility Nano-pipettes, comparatively quantitative analysis performed by cryo-TEM technique.