A5004034582- Bacteriophages and microbial interactions
- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Microbial infections and disease research
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Aquaculture disease management and microbiota
- Herpesvirus Infections and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Hepatitis B Virus Studies
- Plant Virus Research Studies
- Enterobacteriaceae and Cronobacter Research
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Antimicrobial Resistance in Staphylococcus
University of Gdańsk
2015-2022
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2019
Abstract A large scale analysis presented in this article focuses on biological and physiological variety of bacteriophages. collection 83 bacteriophages, isolated from urban sewage able to propagate cells different bacterial hosts, has been obtained (60 infecting Escherichia coli , 10 Pseudomonas aeruginosa 4 Salmonella enterica 3 Staphylococcus sciuri 6 Enterococcus faecalis ). High diversity the is indicated by its characteristics, both morphological (electron microscopic analyses) (host...
The characterization of a recently isolated bacteriophage, vB_Eco4M-7, which effectively infects many, though not all, Escherichia coli O157 strains, is presented. genome this phage comprises double-stranded DNA, 68,084 bp in length, with GC content 46.2%. It contains 96 putative open reading frames (ORFs). Among them, the functions only 35 ORFs were predicted (36.5%), whereas 61 (63.5%) classified as hypothetical proteins. vB_Eco4M-7 does contain genes coding for integrase, recombinase,...
Morphological, biological, and genetic characteristics of a virulent Siphoviridae phage, named vB-EcoS-95, is reported. This phage was isolated from urban sewage. It found to infect some Escherichia coli strains giving clear plaques. The genome this composed 50,910 bp contains 89 ORFs. Importantly, none the predicted ORFs shows any similarity with known pathogenic factors that would prevent its use in medicine. Genome sequence analysis vB-EcoS-95 revealed 74% genomic Shigella pSf-1. Compared...
Phage therapy is a promising alternative treatment of bacterial infections in human and animals. Nevertheless, despite the appearance many strains resistant to antibiotics, these drugs still remain important therapeutics used veterinary medicine. Although experimental phage caused by Salmonella enterica was described previously groups, those studies focused solely on effects bacteriophages. Here, we compared use (employing cocktail composed two isolated characterized bacteriophages,...
Phage therapy is one of main alternative option for antibiotic treatment bacterial infections, particularly in the era appearance pathogenic strains revealing resistance to most or even all known antibiotics. Enterococcus faecalis such pathogens causing serious human infections. In light high level biodiversity bacteriophages and specificity phages species strains, development effective phage depend, between others, on identification characterization a large collection these viruses,...
A microRNA-size (20-nt long) molecule has been identified in Escherichia coli after induction of Shiga toxin-converting bacteriophage Φ24B. This small RNA, named 24B_1, is encoded the lom-vb_24B_43 region phage genome, and apparently it produced by cleavage a larger transcript. devoid 24B_1 revealed decreased efficiency lysogenization, quicker prophage provoking SOS response, higher progeny production during lytic cycle less efficient adsorption on host cells. Expression most genes was...
Apart from antibiotic resistance of pathogenic bacteria, the formation biofilms is a feature that makes bacterial infections especially difficulty to treat. Shiga toxin-producing Escherichia coli (STEC) strains are dangerous pathogens, causing severe in humans, and capable biofilm production. We have reported previously identification characterization vB_Eco4-M7 bacteriophage, infecting various STEC strains. It was suggested this phage might be potentially used therapy against these...
Lambdoid bacteriophages form a group of viruses that shares common schema genome organization and lifecycle. Some them can play crucial roles in creating the pathogenic profiles Escherichia coli strains. For example, Shiga toxin-producing E. (STEC) acquired stx genes, encoding toxins, via lambdoid prophages (Stx phages). The results obtained so far present evidence for relation between exo-xis region phage development, however molecular mechanisms activities genes' products are still...
A newly isolated bacteriophage infecting Enterococcus faecalis strains has been characterized, including determination of its molecular features. This phage, named vB_EfaS-271, classified as a Siphoviridae member, according to electron microscopy characterization the virions, composed 50 nm-diameter head and long, flexible, noncontractable tail (219 × 12.5 nm). Analysis whole dsDNA genome this phage showed that it consists 40,197 bp functional modules containing genes coding for proteins are...
The exo-xis region of lambdoid bacteriophage genomes contains several established and potential genes that are evolutionarily conserved, but not essential for phage propagation under laboratory conditions. Nevertheless, deletion or overexpression either the whole important regulatory elements can significantly influence regulation development. This report defines specific roles orf60a orf61 in λ Φ24B, a Shiga toxin-converting with clinical relevance. We observed mutant phages bearing...
Shiga toxin-converting bacteriophages (Stx phages) are present as prophages in toxin-producing Escherichia coli (STEC) strains. Theses phages can be transmitted to previously non-pathogenic E. cells making them potential producers of toxins, they bear genes for these toxins their genomes. Therefore, sensitivity Stx phage virions various conditions is important both natural processes spreading viruses and prophylactic control appearance novel pathogenic In this report we provide evidence that...
Previous studies indicated that these genetic elements could be involved in the regulation of lysogenization and prophage induction processes. The effects were dramatic Shiga toxin-converting phage Φ24(B) after treatment with oxidative stress-inducing agent, hydrogen peroxide, while they less pronounced bacteriophage λ both phages irradiated UV. peroxide-caused was found to RecA-dependent. Importantly, peroxide-treated E. coli cells lysogenic for either or Φ24(B), deletion exo-xis region...
Abstract The exo-xis region of lambdoid phages contains open reading frames and genes that appear to be evolutionarily important. However, this has received little attention up now. In study, we provided evidence ea22 , the largest gene region, favors lysogenic pathway over lytic in contrast other characterized including ea8.5 orf61, orf60a orf63. Our assays also suggest some functional analogies between Ea22 phage integrase protein (Int). While it is unsurprising operates similarly both λ...
Shiga toxin-producing Escherichia coli (STEC) is a group of pathogenic strains responsible for human infections that result in bloody diarrhea and hemorrhagic colitis, often with severe complications. The main virulence factors STEC are toxins encoded by stx genes located genomes toxin-converting bacteriophages (Stx phages). These bacterial viruses clustered the lambdoid family represented phage λ. Here, we report expression orf73 from exo-xis region genome promotes lysogenic pathway...
Shiga toxin-producing Escherichia coli (STEC) can cause severe infections in humans, leading to serious diseases and dangerous complications, such as hemolytic-uremic syndrome. Although cattle are a major reservoir of STEC, the most commonly occurring source human food products (e.g., vegetables) contaminated with cow feces (often due use natural fertilizers agriculture). Since antibiotics against STEC is controversial, other methods for protection contaminations by these bacteria required....
Abstract Lambdoid bacteriophages are excellent models in studies on molecular aspects of virus-host interactions. However, some them carry genes encoding toxins which responsible for virulence pathogenic strains bacteria. Shiga toxin-converting (Stx phages) encode that cause enterohemorrhagic Escherichia coli (EHEC), and their effective production depends Stx prophage induction. The exo-xis region the lambdoid phage genome consists dispensable multiplication under laboratory conditions;...