A5009429574- Retinal Development and Disorders
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Photochromic and Fluorescence Chemistry
- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
La Jolla Bioengineering Institute
2023
University of California, San Diego
2022-2023
Fleet Science Center
2023
In optic neuropathies, including glaucoma, retinal ganglion cells (RGCs) die. Cell transplantation and endogenous regeneration offer strategies for repair, however, developmental programs required this to succeed are incompletely understood. To address this, we explored cellular reprogramming with transcription factor (TF) regulators of RGC development which were integrated into human pluripotent stem (PSCs) as inducible gene cassettes. When the pioneer NEUROG2 was combined RGC-expressed TFs...
Retinogenesis involves the specification of retinal cell types during early vertebrate development. While model organisms have been critical for determining role dynamic chromatin and cell-type specific transcriptional networks this process, an enhanced understanding developing human retina has more elusive due to requirement fetal tissue. Pluripotent stem (PSC) derived organoids offer experimentally accessible solution investigating retina. To investigate cellular molecular changes in...
This protocol is designed to convert human induced pluripotent stem cells (PSCs) into retinal ganglion cell neurons (RGC-iNs) using a doxycycline-inducible polycistronic transcription factor gene cassette containing NEUROG2, ATOH7, ISL1, and POU4F2. The TetO-driven transgene integrated the CLYBL safe harbor site CRISPR-Cas12a ribonucleoprotein. process of generating greatly enhanced by inclusion BMP blocker LDN-193189.