A5054680862- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Bacterial Genetics and Biotechnology
- Viral Infections and Immunology Research
- Academic Publishing and Open Access
- Law, AI, and Intellectual Property
The Ohio State University
2014-2016
Aminoacyl-tRNA synthetases use a variety of mechanisms to ensure fidelity the genetic code and ultimately select correct amino acids be used in protein synthesis. The physiological necessity these quality control different environments remains unclear, as cost vs benefit accurate synthesis is difficult predict. We show that Escherichia coli, non-coded acid produced through oxidative damage significant threat accuracy must cleared by phenylalanine-tRNA synthetase order prevent cellular...
Non-protein amino acids, particularly isomers of the proteinogenic present a threat to proteome integrity if they are mistakenly inserted into proteins. Quality control during aminoacyl-tRNA synthesis reduces non-protein acid incorporation by both substrate discrimination and proofreading. For example phenylalanyl-tRNA synthetase (PheRS) proofreads hydroxylated phenylalanine derivative m-Tyr after its attachment tRNAPhe. We now show in Saccharomyces cerevisiae that PheRS misacylation tRNAPhe...