A5060030918- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Cellular Mechanics and Interactions
- Force Microscopy Techniques and Applications
- Anodic Oxide Films and Nanostructures
- Nanofabrication and Lithography Techniques
- Cancer, Hypoxia, and Metabolism
- Advanced Proteomics Techniques and Applications
- Electron and X-Ray Spectroscopy Techniques
- 3D Printing in Biomedical Research
- Advanced Electron Microscopy Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Mitochondrial Function and Pathology
University of Sheffield
2020-2023
Hypoxia is a feature of neurodegenerative diseases, and can both directly indirectly impact on neuronal function through modulation glial function. Astrocytes play key role in regulating homeostasis within the central nervous system, mediate hypoxia-induced changes response to reduced oxygen availability. The current study performed detailed characterization transcriptomic profile astrocytes vitro. Human were cultured under normoxic (5% CO2, 95% air) or hypoxic conditions (1% O2, 5% 94% N2)...
Expansion microscopy (ExM) is a versatile super-resolution pipeline, leveraging nanoscale biomolecular crosslinking and osmotically driven swelling of hydrogels. Currently, ExM laborious skill-intensive technique, involving manual handling the hydrogels that can compromise integrity gels capacity to track gel isotropy, hence diminishing reproducibility. We have developed 3D-printable microplate system contain entire workflow within each well, enabling in situ image acquisition eliminating...
14 fluorescent NHS esters of different hydrophobicity and charges were shown to differentially label a range subcellular compartments in HeLa cells using the super-resolution imaging technique expansion microscopy.
Abstract Amine-reactive esters of aromatic fluorescent dyes are emerging as imaging probes for nondescript staining cellular and tissue architectures. We characterised the differential patterns 14 dye ester species with varying physical spectral properties in broadly studied human cell line – HeLa. When combined expansion microscopy (ExM), these stains reveal nanoscale features such nuclear proteome, membrane-bound compartments vesicles. Among N-Hydroxysuccinimide (NHS) esters, we observe...
Abstract Expansion microscopy (ExM) is a versatile super-resolution pipeline, leveraging nanoscale biomolecular cross- linking and osmotically driven swelling of hydrogels. In its current implementation, ExM remains laborious skill-intensive technique, involving manual handling the hydrogels that can compromise integrity gel matrix diminish reproducibility. The lack protocols to constrain orientation during this process lends challenges in tracking isotropy or after swelling. We have...