A5066160093- Pluripotent Stem Cells Research
- Spaceflight effects on biology
- Genetics, Aging, and Longevity in Model Organisms
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Space Science and Extraterrestrial Life
- Cardiac Ischemia and Reperfusion
- CRISPR and Genetic Engineering
- Chemotherapy-induced cardiotoxicity and mitigation
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive Biology and Fertility
- Hemoglobin structure and function
- Rheumatoid Arthritis Research and Therapies
- Retinoids in leukemia and cellular processes
- Sperm and Testicular Function
- Muscle Physiology and Disorders
- Neonatal Health and Biochemistry
- Chronic Kidney Disease and Diabetes
- Antioxidant Activity and Oxidative Stress
- Semiconductor materials and devices
- Radiation Effects in Electronics
- Nuclear Receptors and Signaling
- Cell Adhesion Molecules Research
- Integrated Circuits and Semiconductor Failure Analysis
- Adipokines, Inflammation, and Metabolic Diseases
University of Cologne
2016-2023
University Hospital Cologne
2022-2023
Cadila Healthcare (India)
2011-2015
Spectrum Health
2005
Michigan State University
2005
Etoposide (ETP) and anthracyclines are applied for wide anti-cancer treatments. However, the ETP-induced cardiotoxicity remains to be a major safety issue underlying cardiotoxic mechanisms not well understood. This study is aiming unravel profile of ETP in comparison using physiologically relevant human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs). Using xCELLigence real-time cell analyser (RTCA), we found that single high dose induces irreversible increase hPSC-CMs beating rate...
Embryonic developmental studies under microgravity conditions in space are very limited. To study the effects of altered gravity on embryonic development processes we established an vitro methodology allowing differentiation mouse stem cells (mESCs) simulated within a fast-rotating clinostat (clinorotation) and capture microarray-based gene signatures.The differentiating mESCs were cultured 2D pipette clinostat. The microarray bioinformatics tools used to genes that deregulated by their...
Exposure to outer space microgravity poses a risk for the development of various pathologies including cardiovascular disease. To study this, we derived cardiomyocytes (CMs) from human-induced pluripotent stem cells and exposed them simulated (SMG). We combined different "omics" chromosome conformation capture technologies with live-cell imaging transgenic lines discover that SMG impacts on contractile velocity function CMs via induction senescence processes. This is linked SMG-induced...
Retinol-binding protein 4 (RBP4) is an adipocyte-secreted hormone proposed to link obesity with insulin resistance. However, the role of RBP4 in cardiovascular complications yet be fully understood. The present study aimed decipher association between pro-inflammatory cytokines and low-density lipoprotein (LDL) cholesterol diet-induced obese hyperlipidaemic mice. To understand correlation, rimonabant, anti-obesity drug, has been used relieve atherosclerotic predisposition.Adipose and/or...
Embryonic developmental studies under microgravity conditions in space are very limited. To study the effects of short-term altered gravity on embryonic development processes, we exposed mouse stem cells (mESCs) to phases hypergravity and studied differentiation potential using wide-genome microarray analysis. During 64th European Space Agency's parabolic flight campaign, mESCs were 31 parabolas. Each parabola comprised lasting 22 s hypergravity, microgravity, a repeat hypergravity. On...
Functional studies of human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes (hCMs) under different gravity conditions contribute to aerospace medical research. To study the effects altered on hCMs, we exposed them acute hypergravity and microgravity phases in presence absence β-adrenoceptor isoprenalin (ISO), L-type Ca2+ channel (LTCC) agonist Bay-K8644, or LTCC blocker nifedipine, monitored their beating rate (BR). These logistically demanding experiments were executed during...
Live-cell imaging techniques are essential for acquiring vital physiological and pathophysiological knowledge to understand treat heart disease. For live-cell of transient alterations [Ca2+]i in human cardiomyocytes, we engineered human-induced pluripotent stem cells carrying a genetically-encoded Ca2+-indicator (GECI). To monitor sarcomere shortening relaxation cardiomyocytes real-time, generated α-cardiac actinin (ACTN2)-copepod (cop) green fluorescent protein (GFP+)-human-induced cell...
Application of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) is limited by the challenges in their efficient differentiation. Recently, Wingless (Wnt) signaling pathway has emerged as key regulator cardiomyogenesis. In this study, we evaluated effects cyclooxygenase inhibitors on cardiac differentiation hPSCs. Cardiac was performed adherent monolayer based method using 4 hPSC lines (HES3, H9, IMR90, and ES4SKIN). The efficiency flow cytometry RT-qPCR. Generated hPSC-CMs were...
<b>Objectives:</b> The aim was to investigate the nephroprotective effect of combination aliskiren (ASK), a direct renin inhibitor and pentoxifylline (PTX), tumor necrotic factor-alpha (TNF-alpha), in rat remnant kidney model chronic disease (CKD).<br> <b>Materials</b> <b>and</b> <b>Methods:</b> Nephrectomized (NPX) rats were treated with ASK (10 mg/kg, p.o.), PTX (100 + once daily for 28 days. We have performed analysis various renal injury parameters after 4 weeks treatment.<br>...
Animal studies for embryotoxicity evaluation of potential therapeutics and environmental factors are complex, costly, time-consuming. Often, not human relevance because species differences. In the present study, we recapitulated process cardiomyogenesis in induced pluripotent stem cells (hiPSCs) by modulation Wnt signaling pathway to identify a key gene signature that can be applied compounds and/or stress compromising process. Among 23 tested teratogens 16 non-teratogens, identified three...
Abstract Animal studies for embryotoxicity evaluation of potential therapeutics and environmental factors are complex, costly, time-consuming. Often, not human relevance because species differences. In the present study, we recapitulated process cardiomyogenesis in induced pluripotent stem cells (hiPSCs) by modulation Wnt signaling pathway to identify a key gene signature that can be applied compounds and/or stress compromising process. Among 23 tested teratogens 16 non-teratogens,...