A5014700294- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Lipid Membrane Structure and Behavior
- Light effects on plants
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Neural dynamics and brain function
- Photosynthetic Processes and Mechanisms
- Advanced biosensing and bioanalysis techniques
- Nicotinic Acetylcholine Receptors Study
- Molecular Sensors and Ion Detection
- Spectroscopy and Quantum Chemical Studies
- Advanced Fluorescence Microscopy Techniques
- Receptor Mechanisms and Signaling
- Connexins and lens biology
- Bacterial Genetics and Biotechnology
- Enzyme function and inhibition
- Neuroscience and Neural Engineering
- bioluminescence and chemiluminescence research
- Heat shock proteins research
- Forensic Fingerprint Detection Methods
- thermodynamics and calorimetric analyses
- Mass Spectrometry Techniques and Applications
- Plant Molecular Biology Research
- Supramolecular Chemistry and Complexes
St George's, University of London
2017-2023
Freie Universität Berlin
2013-2017
Bielefeld University
2008
Significance Excitatory synapses convert presynaptic action potentials into chemical signals that are sensed by postsynaptic glutamate receptors. To eavesdrop on synaptic transmission, genetically encoded fluorescent sensors for have been developed. However, even the best available lag behind very fast dynamics in cleft. Here, we report development of an ultrafast sensor, iGlu u , which allowed us to image clearance and depression during 100-Hz spike trains. We found only boutons showing...
Abstract Sensor histidine kinases are central to sensing in bacteria and plants. They usually contain sensor, linker, kinase modules the structure of many these components is known. However, it unclear how module structurally regulated. Here, we use nano- millisecond time-resolved X-ray scattering visualize solution structural changes that occur when light-sensitive model YF1 activated by blue light. We find coiled coil linker attached domains undergo a left handed rotation within...
Proper insertion, folding and assembly of functional proteins in biological membranes are key processes to warrant activity a living cell. Here, we present novel approach trace insertion nascent membrane protein leaving the ribosome penetrating bilayer. Surface Enhanced IR Absorption Spectroscopy selectively monitored during cell-free expression label-free non-invasive manner. Protein synthesis was performed an optical cell containing prism covered with thin gold film nanodiscs on top,...
Channelrhodopsin‐1 from Chlamydomonas augustae ( Ca ChR1) is a light‐activated cation channel, which promising optogenetic tool. We show by resonance Raman spectroscopy and retinal extraction followed high pressure liquid chromatography (HPLC) that the isomeric ratio of all‐ trans to 13‐ cis solubilized channelrhodopsin‐1 with 70:30 identical channelrhodopsin‐2 reinhardtii Cr ChR2). Critical frequency shifts in vibrations are identified spectrum upon transition open (conductive P 2 380 )...
Aureochrome 1 from Vaucheria frigida is a recently identified blue-light receptor that acts as transcription factor. The protein comprises photosensitive light-, oxygen- and voltage-sensitive (LOV) domain basic zipper (bZIP) binds DNA rendering aureochrome prospective optogenetic tool. Here, we studied the photoreaction of full-length by molecular spectroscopy. kinetics decay red-shifted triplet state blue-shifted signaling were determined time-resolved UV/Vis It shown presence bZIP further...
Ca2+/calmodulin (Ca2+/CaM) interaction with connexins (Cx) is well-established; however, the mechanistic basis of regulation gap junction function by Ca2+/CaM not fully understood. predicted to bind a domain in C-terminal portion intracellular loop (CL2) vast majority Cx isoforms and for number Cx-s this prediction has proved correct. In study, we investigate characterise both apo-CaM binding selected representatives each α, β γ connexin family develop better understanding CaM effects on...
Abstract New luminescent 1‐aza‐15‐crown‐5 ethers bearing a coumarin unit were synthesised leading to the cavitand‐based calix crown ether 9 . The solid‐state structures of 5 and 6 sodium potassium iodide complexes are presented. association constants for complex formation with alkali metal ions in methanol determined. Only weak formed ( K Na = 286 M –1 , 392 ). aza‐15‐crown‐5 was attached by methylene cavitand give new fluorescent investigated as well, an enhancement constant found 1215 497...
Abstract The recently discovered photo‐activated adenylyl cyclase ( mPAC from Microcoleus chthonoplastes) is the first PAC that owes a light‐, oxygen‐ and voltage‐sensitive LOV ) domain for blue‐light sensing. photoreaction of receptor was studied by time‐resolved UV /vis light‐induced Fourier transform infrared FTIR absorption difference spectroscopy. photocycle comprises typical triplet state 715 thio‐adduct 390 . While adduct decays with time constant 8 s, lifetime 656 ns significantly...
ABSTRACT Glutamatergic synapses display a rich repertoire of plasticity mechanisms on many different time scales, involving dynamic changes in the efficacy transmitter release as well number and function postsynaptic glutamate receptors. The genetically encoded sensor iGluSnFR enables visualization from presynaptic terminals at frequencies up to ∼10 Hz. However, resolve dynamics during high frequency bursts, faster indicators are required. Here we report development fast (iGlu f ) ultrafast...
ABSTRACT Protein-based fluorescent glutamate sensors have the potential for real-time monitoring of synaptic and cellular concentration changes, however even fastest currently available sensors’ response times 2-3 ms are too slow accurate reporting post-synaptic AMPA receptor function in physiological conditions. We developed probes based on bacterial periplasmic glutamate/aspartate binding protein with either an endogenously or a synthetic fluorophore as indicator binding: affinity variants...