- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Additive Manufacturing and 3D Printing Technologies
- Molecular Communication and Nanonetworks
- Bone Tissue Engineering Materials
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Planarian Biology and Electrostimulation
- Plant and Biological Electrophysiology Studies
- Cardiovascular Issues in Pregnancy
- Erythrocyte Function and Pathophysiology
- Spaceflight effects on biology
- Force Microscopy Techniques and Applications
- Cancer Cells and Metastasis
- Ion channel regulation and function
- Osteoarthritis Treatment and Mechanisms
- Diffusion and Search Dynamics
- Tendon Structure and Treatment
- Advanced Cellulose Research Studies
- Nanoparticle-Based Drug Delivery
- Chemical and Physical Studies
- Extracellular vesicles in disease
- Photoreceptor and optogenetics research
University of Maryland, College Park
2016-2019
Princeton University
2012-2017
In the past few decades, field of tissue engineering combined with rapid prototyping (RP) techniques has been successful in creating biological substitutes that mimic tissues. Its applications regenerative medicine have drawn efforts research from various scientific fields, diagnostics, and clinical translation to therapies. While some areas therapeutics are well developed, such as skin replacement, many others cartilage repair can still greatly benefit RP due low success and/or inefficiency...
Human mesenchymal stem/stromal cells (hMSCs) have been investigated and proven to be a well-tolerated, safe therapy for variety of indications, as shown by over 900 registered hMSC-based clinical trials. To meet the commercial demand manufacturing hMSCs, production requires scale that can achieve lot size ~100B cells, which innovative technologies such 3D bioreactors. A robust suspension bioreactor process scaled-up relevant is therefore crucial. In this study, we developed fed-batch,...
Stem cell cultures within perfusion bioreactors, while efficient in obtaining numbers, often lack the similarity to native tissues and consequently phenotype. We develop a three-dimensional (3D)-printed fluidic chamber for dynamic stem culture, with emphasis on control over flow substrate curvature 3D environment, two physiologic features of tissues. The geometry, consisting an array vertical cylindrical pillars, facilitates actin-mediated localization human mesenchymal cells (hMSCs) ∼200 μm...
Although the process of chemosensing by individual cells is intrisically stochastic, multicellular organisms exhibit highly regulated responses to external stimulations. Two key elements understand deterministic features are intercellular communications and role pacemaker cells. To characterize collective behavior induced these two factors, we study spatial-temporal calcium dynamics fibroblast in response ATP stimulation. We find that closely packed cell colonies faster, more synchronized,...
Cells constantly sense their chemical and mechanical environments. We study the effect of mechanics on ATP-induced collective calcium response fibroblast cells in experiments that mimic various tissue find closely packed two-dimensional cell cultures a soft polyacrylamide gel (Young's modulus E = 690 Pa) contain more exhibiting oscillations than rigid substrate (E 36 000 Pa). Calcium responses substrates show slower decay level relative to those substrates. Actin enhancement disruption for...
The maintenance of tissue integrity is essential for the life multicellular organisms. Healing a skin wound paradigm how various cell types localize and repair perturbations in an orchestrated fashion. To investigate biophysical mechanisms associated with localization, we focus on model system consisting fibroblast monolayer elastic substrate. We find that creation edge causes cytosolic calcium oscillations throughout monolayer. oscillation frequency increases density, which shows...
Abstract There is a growing interest in 3D printing to fabricate culture substrates; however, the surface properties of scaffold remain pertinent elicit targeted and expected cell responses. Traditional 2D polystyrene (PS) systems typically require functionalization (oxidation) facilitate encourage adhesion. Determining which enhance protein adhesion from media cellular extracellular matrix (ECM) production remains first step translating PS surface. Here we show that presence carbonyl groups...
ABSTRACT The maintenance of tissue integrity is essential for the life multicellular organisms. Healing a skin wound paradigm how various cell types localize and repair perturbations in an orchestrated fashion. To investigate biophysical mechanisms associated with localization, we focus on model system consisting fibroblast monolayer elastic substrate. We find that creation edge causes cytosolic calcium oscillations throughout monolayer. oscillation frequency increases density, which shows...