Sleep is thought to be important for memory consolidation, since sleep deprivation has been shown interfere with processing. However, the effects of augmenting on formation are not well known, and testing role in enhancement limited pharmacological behavioral approaches. Here we test effect overexpressing brain-type fatty acid binding protein (Fabp7) long-term (LTM) Drosophila melanogaster. Transgenic flies carrying murine Fabp7 or homologue dFabp had reduced baseline but normal LTM, while...

Sleep contributes to cognitive functioning and is sufficient alter brain morphology function. However, mechanisms underlying sleep regulation remain poorly understood. In mammals, tumor necrosis factor-alpha (TNFα) known regulate sleep, cytokine expression may represent an evolutionarily ancient mechanism in regulation. Here we show that the Drosophila TNFα homologue, Eiger, mediates flies. We knockdown of Eiger astrocytes, but not neurons, significantly reduces duration, total...

The astrocyte brain fatty acid binding protein (Fabp7) has previously been shown to have a coordinated diurnal regulation of mRNA and throughout mouse brain, an age-dependent decline in expression within synaptoneurosomal fractions. Mechanisms that control time-of-day changes trafficking Fabp7 the perisynaptic process are not known. In this study, we confirmed enrichment astrocytic compartment, observed change intracellular distribution molecular layers hippocampus. Northern blotting...

Given the relationship between sleep and plasticity, we examined role of Extracellular signal-regulated kinase (ERK) in regulating baseline sleep, modulating response to waking experience. Both deprivation social enrichment increase ERK phosphorylation wild-type flies. The effects both on structural plasticity LNvs can be recapitulated by expressing an active version (UAS-ERK(SEM)) pan-neuronally adult fly using GeneSwitch (Gsw) Gsw-elav-GAL4. Conversely, disrupting reduces prevents...

Disruption of sleep/wake activity in Alzheimer's disease (AD) patients significantly affects their quality life and that caretakers is a major contributing factor for institutionalization. Levels amyloid-β (Aβ) have been shown to be regulated by neuronal correlate with the cycle. Whether consolidated sleep can disrupted Aβ alone not well understood. We hypothesize Aβ42 increase wakefulness disrupt sleep. Here we report flies expressing human transgene neurons reduced compared control flies....

Fatty acid binding proteins (FABPs) are a family of intracellular lipid chaperone known to play critical roles in the regulation fatty uptake and transport as well gene expression. Brain-type protein (FABP7) is enriched astrocytes has been implicated sleep/wake neurodegenerative diseases; however, precise mechanisms underlying role FABP7 these biological processes remain unclear. binds both arachidonic (AA) docosahexaenoic (DHA), resulting discrete physiological responses. Here, we propose...

It has long been known that central opioid systems play an important role in certain aspects of appetite and food intake, particularly with regard to the hedonic or rewarding impact calorically dense food, such as fat sugar. Ventral striatal enkephalin may be a key component this system, infusions mu-opiate agonists into region strongly increase feeding, whereas opiate antagonists decrease intake. While pharmacological analysis consistently supported role, direct measurement gene expression...

We recently reported evidence implicating fatty-acid binding protein (Fabp) in the control of sleep and memory formation. used Drosophila melanogaster to examine relationship between through transgenic overexpression mouse brain-Fabp, Fabp7, or Fabp homologue, (dFabp). The key findings are that, (1) a genetically induced increase daytime consolidated (naps) correlates with an cognitive performance, (2) late "window" consolidation occurs days after traditionally understood "synaptic"...

We recently reported evidence implicating fatty-acid binding protein (Fabp) in the control of sleep and memory formation. used Drosophila melanogaster to examine relationship between through transgenic overexpression mouse brain-Fabp, Fabp7, or Fabp homolog, (dFabp). The key findings are that 1) a genetically induced increase daytime consolidated (naps) correlates with an cognitive performance, 2) late "window" consolidation occurs days after traditionally understood "synaptic"...

The astrocyte brain-type fatty-acid binding protein (Fabp7) circadian gene expression is synchronized in the same temporal phase throughout mammalian brain. Cellular and molecular mechanisms that contribute to this coordinated are not completely understood, but likely involve nuclear receptor Rev-erbα (NR1D1), a transcriptional repressor. We performed ChIP-seq on ventral tegmental area (VTA) identified targets of Rev-erbα, including Fabp7. confirmed binds Fabp7 promoter multiple brain areas,...

Abstract Sleep disturbances are commonly found in trauma-exposed populations. Additionally, trauma exposure results fear-associated memory impairments. Given the interactions of sleep with learning and memory, we hypothesized that increasing duration following would restore overall function improve trauma-induced dysfunction. Here, utilized single prolonged stress, a validated rodent model post-traumatic stress disorder, combination optogenetic activation hypothalamic melanin-concentrating...

Parkinson's Disease (PD) is the most common movement disorder, and strongest genetic risk factor for PD mutations in glucocerebrosidase gene (GBA). Mutations GBA also lead to development of Gaucher (GD), type lysosomal storage disorder. Current therapeutic approaches fail address neurological GD symptoms. Therefore, identifying strategies that improve phenotypic traits associated with GD/PD animal models may provide an opportunity treating manifestations GD/PD. Thiazolidinediones (TZDs,...

Humans with post-traumatic stress disorder (PTSD) exhibit sleep disturbances that include insomnia, nightmares, and enhanced daytime sleepiness. Sleep are considered a hallmark feature of PTSD; however, little is known about the cellular molecular mechanisms regulating trauma-induced disorders. Using rodent model PTSD called "Single Prolonged Stress" (SPS) we examined requirement brain-type fatty acid binding protein Fabp7, an astrocyte expressed lipid-signaling molecule, in mediating...

Sleep is a behavior that exists broadly across animal phyla, from flies to humans, and necessary for normal brain function. Recent studies in both vertebrates invertebrates have suggested role glial cells sleep regulatory processes. Changes neural-glial interactions been shown be critical synaptic plasticity circuit Here, we wanted test the hypothesis changes pressure alters interactions. In fruit fly, Drosophila melanogaster, known regulated by mushroom body (MB) circuits. We used technique...

Sleep is intimately linked to cognitive performance and exposure traumatic stress that leads post-traumatic disorder (PTSD) impairs both sleep function. However, the contribution of pre-trauma loss subsequent trauma-dependent fear-associated memory impairment remains unstudied. We hypothesized deprivation (SD) prior trauma may increase severity a PTSD-like phenotype in rats exposed single prolonged (SPS), rodent model PTSD. Rats were SPS alone, SD or combination SPS+SD measures impairments...

Physical exercise and fitness may serve as resilience factors to stress exposure. However, the extreme range in human performance suggests that genetic variation for capacity could be a confounding feature understanding connection between To test this idea, we use laboratory rat models selectively bred low high gain aerobic running response training examine whether an inherent respond physical reflects how changes neurobiological functioning regulates fear-associated memory processing....

Although humans spend a third of life asleep, the function this sleep serves is still unknown. Using model system Drosophila melanogaster, we have begun to identify genes that alter either or homeostasis, increase in observed after loss. One identified, extra‐cellular signal regulated kinase ( ERK ), particular interest given its role synaptic plasticity. Thus, expressed an activated form UAS‐ERK act ) adult flies using pan neuronal Geneswitch driver Gsw‐elav . We found RU486 (RU) fed...