A5018986182- Molecular Sensors and Ion Detection
- Luminescence and Fluorescent Materials
- Planarian Biology and Electrostimulation
- Hippo pathway signaling and YAP/TAZ
- Monoclonal and Polyclonal Antibodies Research
- Analytical Chemistry and Sensors
- Plant and Biological Electrophysiology Studies
- SARS-CoV-2 detection and testing
- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- Biosensors and Analytical Detection
- Biochemical Analysis and Sensing Techniques
- Advanced biosensing and bioanalysis techniques
- Cancer Cells and Metastasis
- Olfactory and Sensory Function Studies
- RNA modifications and cancer
- RNA Research and Splicing
- Neurobiology and Insect Physiology Research
- bioluminescence and chemiluminescence research
- Photoreceptor and optogenetics research
- Fibroblast Growth Factor Research
- Crystallography and molecular interactions
- X-ray Diffraction in Crystallography
- Epigenetics and DNA Methylation
- Marine Ecology and Invasive Species
Institute for Stem Cell Biology and Regenerative Medicine
2017-2025
University of Hyderabad
2020-2025
National Centre for Biological Sciences
2017-2020
University of Trans-Disciplinary Health Sciences and Technology
2020
University of North Carolina at Chapel Hill
2010-2019
Indian Institute of Science Bangalore
2002-2018
Article12 June 2019Open Access Transparent process Dynamic expression of tRNA-derived small RNAs define cellular states Srikar Krishna Centre for Inflammation and Tissue Homeostasis, Institute Stem Cell Science Regenerative Medicine, Bangalore, India Technologies the Advancement Science, SASTRA University, Thirumalaisamudram, Thanjavur, Search more papers by this author Daniel GR Yim orcid.org/0000-0001-6153-4725 Precision Oncology, Genome Singapore, Singapore City, Vairavan Lakshmanan...
The inner mitochondrial membrane (IMM), housing components of the electron transport chain (ETC), is site for respiration. ETC relies on mobile carriers; therefore, it has long been argued that fluidity densely packed IMM can potentially influence flux and cell physiology. However, unclear if cells temporally modulate upon metabolic or other stimulation. Using a photostable, red-shifted, cell-permeable molecular-rotor, Mitorotor-1, we present multiplexed approach quantitatively mapping in...
Bright, sensitive fluorescent probes that respond to changes in the cellular microenvironment are extremely valuable for imaging dynamics. We report a simple, one-step synthesis of new hemicaynine (HC-1) dye as sensitive, red-emitting (λmax-610 nm) fluorogenic probe micro-viscosity and local order diverse environments, including live cells. HC-1 responds increasing through fluorescence intensity lifetime, is enough dynamic micellar self-assembly. While shows properties molecular 'rotor',...
Light matters! Flatworms reveal stunning complexity in light-induced behaviors and comparative processing.
An easy to synthesize amphiphilic dye is developed whose sensing behavior in surfactant assemblies can be modulated through surface-charge, micropolarity, and local pH, etc. Thus, the micelle-bound probe shows remarkable ion-dependent bathochromic shifts charge-transfer band, enabling simultaneous ratiometric detection of four different metal ions, such as Cu2+, Ni2+, Hg2+, Zn2+, at parts per billion (ppb) level water. This indeed a striking observation since naked-eye multiple ions...
Animal light sensing and processing are intimately connected to the design of sensory apparatus, with eye-design a subject longstanding interest. Understanding animal response stimuli can elucidate nature neural-processing encoded within photosensory system, tuned specific eye-design. Planarians show exquisite photosensitivity remarkably diverse light-induced behaviors. Further, they bilaterally-symmetric possess prototypic cup-shaped eyes, considered an important landmark in eye evolution....
Membrane-driven self-assembly of an amphiphilic pyrene–terpyridine probe efficiently reports on vesicle–micelle transition through ratiometric changes.
Dyes with environment-sensitive fluorescence have proven useful to study the spatiotemporal dynamics of protein activity in living cells. When attached proteins, their can reflect conformational changes, post-translational modifications, or interactions. However, utility such dye-protein conjugates has been limited because it is difficult load them into They usually must be introduced using techniques that perturb cell physiology, limit throughput, generate fluorescent vesicles (e.g.,...
Environment-sensitive molecular probes offer the potential for a comprehensive mapping of complex cellular milieu. We present here radically new strategy multiplexing highly sensitive, spectrally tuned fluorescent dyes sensing microenvironment. To achieve this multicolor, ratiometric imaging, we first developed series tunable rotors mitochondrial with emission wavelengths spanning visible spectrum. These fluorogenic merocyanine are all sensitive to solvent viscosity despite distinctive...
Monitoring dynamic changes in mitochondrial pH live cells using pH-sensitive fluorescent probe.
Excitation-dependent tuning of the emission behavior fluorescent organic nanoparticles (FONs) with two simple luminescent pyrenyl-pyridyl conjugates as model systems is demonstrated. In case compound a flexible bis-picolyl moiety, simultaneous presence multiple ground-state species distinct absorption and characteristics can be observed. The relative ratios these easily modulated, it possible to selectively stimulate any one them individually by choosing an appropriate excitation channel....
FMRP is an RNA-binding protein that known to localize in the cytoplasm and nucleus. Here, we have identified interaction of with a specific set C/D box snoRNAs guide 2'O methylations ribosomal RNA (rRNA) on defined sites, this modification regulates rRNA folding assembly ribosomes. methylation partial several sites human embryonic stem cells, which results ribosomes differential patterns. FMRP-snoRNA affects these absence FMRP, pattern significantly altered. We found recognizes carrying...
The continued resurgence of the COVID-19 pandemic with multiple variants underlines need for diagnostics that are adaptable to virus. We have developed toehold RNA-based sensors across SARS-CoV-2 genome direct and ultrasensitive detection virus its prominent variants. Here, isothermal amplification a fragment RNA coupled activation our biosensors leads conformational switch in sensor. This translation reporter protein, example, LacZ or nano-lantern is easily detected using...
We present a simple but unique, new probe 1-pyrenyl-methyl-bis(2-picolyl) amine (Pybpa) that selectively senses interfacially bound Zn2+ ions while being completely silent towards in bulk water.
The ability to respond light has profoundly shaped life. Animals with eyes overwhelmingly rely on their visual circuits for mediating light-induced coordinated movements. Building previously reported behaviors, we report the discovery of an organized, eye-independent (extraocular), body-wide photosensory framework that allows even a head-removed animal move like intact animal. Despite possessing sensitive cerebral and centralized brain controls most planarians show acute, ultraviolet-A...
We report on the ability of a new fluorescent probe, 4‐(2‐pyren‐1‐yl‐vinyl) pyridine, 1, to respond micelles and phospholipid vesicles different surface charge. The probe gets incorporated into micellar membranous assemblies shows large red‐shift in fluorescence emission maxima especially when charge organized media is anionic. effect photo‐excitation very clear pronounced as it can be easily visualized. sample color upon changes from blue orange/red once experiences negatively charged...
Euchromatic histone methyltransferases (EHMTs), members of the KMT1 family, methylate and non-histone proteins. Here, we uncover a novel role for EHMTs in regulating heterochromatin anchorage to nuclear periphery (NP) via methylation. We show that stabilize LaminB1 (LMNB1), which associates with H3K9me2-marked peripheral heterochromatin. Loss LMNB1 methylation or abrogates at NP further demonstrate loss induces many hallmarks aging including global reduction H3K27methyl marks altered...
Preservation of a small population cancer stem cells (CSCs) within heterogeneous carcinoma serves as paradigm to understand how select in tissue maintain their undifferentiated status. In both embryogenesis and cancer, Snail has been correlated with stemness, but the molecular underpinning this phenomenon remains largely ill-defined. models cutaneous squamous cell (cSCC), we discovered non-epithelial-mesenchymal transition function for transcription factor maintaining stemness epidermal...
Extracellular matrix (ECM) is an important component of stem cell niche. Remodeling ECM mediated by regulators, such as metalloproteinases (MMPs) plays a vital role in function. However, the mechanisms that modulate function regulators niche are understudied. Here, we explored transcription factor (TF) ETS-1, which expressed cathepsin-positive population, regulating expression regulator, mt-mmpA, thereby modulating basement membrane thickness. In planarians, around gut/inner parenchyma...
Cell behavior is controlled through spatio-temporally localized protein activity. Despite unique and often contradictory roles played by Src-family-kinases (SFKs) in regulating cell physiology, activity patterns of individual SFKs have remained elusive. Here, we report a biosensor for specifically visualizing active conformation SFK-Fyn live cells. We deployed combinatorial library screening to isolate binding-protein (F29) targeting activated Fyn. Nuclear-magnetic-resonance (NMR) analysis...