Adrenomedullin (AM) is an important regulatory peptide involved in both physiological and pathological states. We have previously demonstrated the existence of a specific AM-binding protein (AMBP-1) human plasma. In present study, we developed nonradioactive ligand blotting assay, which, together with high pressure liquid chromatography/SDS-polyacrylamide gel electrophoresis purification techniques, allowed us to isolate AMBP-1 homogeneity. The purified was identified as complement factor H....

The effects of nutrition on plasma concentrations insulin-like growth factor-I (IGF-I) were characterized in steers under basal conditions and following single i.m. injection bovine hormone (bGH, .1 mg/kg BW). Nutritional IGF-I studied three trials. In all trials individually fed penned Angus or Hereford x (280 kg). the first trial, two diets (LPLE1: 8% CP 1.96 Mcal ME/kg, 4.5 kg.hd-1.d-1; MPHE1: 11% CP, 2.67 6.5 kg.hd-1.d-1) (n = 5/diet). Plasma averaged 74 (LPLE1) 152 (MPHE1) ng/ml (P less...

Adrenomedullin (AM), a recently discovered hypotensive peptide, is expressed in the endocrine pancreas of different species, as demonstrated by immunocytochemistry. Electron microscopic studies with double immunogold showed colocalization AM and pancreatic polypeptide. A homogeneous expression receptor was found throughout islet using situ hybridization. Six insulin- producing cell lines have been analyzed reverse transcription-PCR both its receptor. Two experimental models used to study...

To test adrenal responsiveness of beef cattle to stress, plasma norepinephrine, epinephrine, and cortisol responses Angus x Hereford weaning were measured in blood samples taken from 1) eight cows their 4- 6-mo-old calves before after separation and, the following day, being reunited 2) an equal number control animals. After they reunited, dams returned original pen adjacent pen. Experimental trials conducted every 2 wk included two cow/calf pairs per trial. Norepinephrine concentrations...

Endotoxin, a potent stimulator of the immune system and an important mediator in pathophysiology septic shock, has been shown to alter release certain hormones following its systemic administration. The purpose this study was determine effects endotoxin on pituitary hormone secretion both vivo vitro sheep, with emphasis placed growth (GH) release. Endotoxin (400 ng/kg i.v.) increased plasma GH, adrenocorticotropic (ACTH), cortisol prolactin, while it decreased luteinizing (LH) pulse...

Beyond their nutritional effect, short-chain fatty acids, especially butyrate, modulate cell differentiation, proliferation, motility, and in particular, they induce cycle arrest apoptosis. A bovine kidney epithelial line (Madin-Darby kidney; MDBK) was used to investigate the regulatory apoptotic effects of butyrate. Butyrate not only induced apoptosis but also at G1/S boundary M/G2 MDBK cells (P < 0.01). The responses were concentration-dependent (r2 = 0.9482, P <0.001). In examining...

Frequently, peptide hormones circulate in plasma associated with specific binding proteins that modify the clearance and biochemical activities of peptide. Our experimental approach was to use 125I-ligand blotting procedures probe for presence adrenomedullin (AM) (AMBPs). Plasma from chick, calf, dog, goat, guinea pig, human, mouse, rabbit sheep blood were separated electrophoretically 10% nonreducing SDS-polyacrylamide gels transferred nitrocellulose. Nonspecific tracer blocked on...

The goal of this research was to determine whether directing expression an insulin-like growth factor I (IGF-I) transgene specifically striated muscle would alter the characteristics in swine. Transgenic pigs were produced with a fusion gene composed avian skeletal alpha-actin regulatory sequences and cDNA encoding human IGF-I. Six founder transgenic mated nontransgenic produce 11 litters G1 sibling control progeny. Birth weight, weaning proportion pig survival did not differ between pigs....

Abstract Endotoxemia and sepsis cause severe shifts in metabolism towards catabolic events. The objective of the research was to determine whether endotoxin administration changes plasma concentrations IGF-I IGF-binding protein-2 (IGFBP-2) Angus × Hereford steers. In Experiment 1, mean feed intake endotoxin-treated steers ( n =6) decreased 60% within first 24 h after averaged, for duration 96 test period, an 35·5% lower than ad libitum recorded prior endotoxin. Plasma averaged 182 ng/ml...

Abstract The present study defined the effects of GH administration on components nitric oxide (NO)-generating cascade to account for observed increases in NO production and protein nitration after an immune challenge. Calves were assigned groups with or without treatment (100 μg GH/kg body weight placebo im, daily 12 d) low-level endotoxin [lipopolysaccharide (LPS), 2.5 μg/kg, placebo, iv]. Plasma was obtained estimation changes as [NO2− + NO3−] (NOx). Transcutaneous liver biopsies...

Effects of dietary protein level with and without L-arginine (Arg) infusion on plasma tumor necrosis factor-β (TNF-β) response to endotoxin (lipopolysaccharide [LPS]) as well concentration urine output nitrite nitrate (NOx), the stable end products nitric oxide radical (NO), were studied in beef heifers (275-310 kg body wt). The animals fed low- (LP; 7.96%) or high- (HP; 13.94%) diets for 10 days before LPS administration (Escherichia coli; 0.2 μg/kg, iv). L-Arginine saline (0.5 g/kg wt) was...

A generalized increase in liver protein tyrosine nitration (3′-nitrotyrosine, 3′-NT) occurs after GH injection a time frame consistent with observed acute hyporesponsiveness. Here we investigated whether the GH-associated process might be targeted to 1007Y-1008Y-phosphorylation epitope of Janus kinase (JAK)-2 because its homology defined peptide motif. Using antibodies developed 3′NT-substituted analog 1007Y-1008Y-JAK2 site (nitro-JAK2), demonstrated rapid membrane-associated nitro-JAK2 GH....

Twenty crossbred beef steers (initial BW 182 ± 1.8 kg) were used in a 2 × factorial to determine the effects of implantation with Synovex-S® (SYN; 20 mg 17-β estradiol benzoate + 200 progesterone, ear implant) and administration recombinant bovine growth hormone (Somavubove® SbV, .1 mg·kgBW−1·d−1, i.m.) on morphology six muscles (longissimus, psoas major, supraspinatus, triceps brachii, semitendinosus, rectus femoris) growing steers. Implantation SYN decreased percentage distribution FOG...