A5102027541- Immune Cell Function and Interaction
- Hematopoietic Stem Cell Transplantation
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Reproductive System and Pregnancy
- T-cell and B-cell Immunology
- Antimicrobial Peptides and Activities
- Prenatal Screening and Diagnostics
- Chemokine receptors and signaling
- Mesenchymal stem cell research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Pediatric health and respiratory diseases
- Immune Response and Inflammation
- Endometriosis Research and Treatment
- Antifungal resistance and susceptibility
- Genetic Syndromes and Imprinting
- Nitric Oxide and Endothelin Effects
- Cancer, Hypoxia, and Metabolism
- Adrenal and Paraganglionic Tumors
- S100 Proteins and Annexins
- IL-33, ST2, and ILC Pathways
- Tumors and Oncological Cases
- Glioma Diagnosis and Treatment
- Pregnancy and preeclampsia studies
- Neuroblastoma Research and Treatments
Cellular Therapeutics (United Kingdom)
2025
National Institutes of Health
2009-2024
National Heart Lung and Blood Institute
2024
National Center for Advancing Translational Sciences
2023
National Institute of Environmental Health Sciences
2022
Beijing Hospital of Traditional Chinese Medicine
2020
National Institute of Allergy and Infectious Diseases
2009-2018
Heat Biologics (United States)
2016-2017
We sought to define protective mechanisms of immunity Staphylococcus aureus and Candida albicans bloodstream infections in mice immunized with the recombinant N-terminus Als3p (rAls3p-N) vaccine plus aluminum hydroxide (Al(OH3) adjuvant, or adjuvant controls. Deficiency IFN-γ but not IL-17A enhanced susceptibility control both infections. However, vaccine-induced against required CD4+ T-cell-derived IL-17A, functional phagocytic effectors. Vaccination primed Th1, Th17, Th1/17 lymphocytes,...
Abstract It was reported that host defense against pulmonary Klebsiella pneumoniae infection requires IL-22, which proposed to be of T cell origin. Supporting a role for we found Il22−/− mice had decreased survival compared with wild-type after intratracheal K. pneumoniae. Surprisingly, however, Rag2−/− did not differ from in or levels IL-22 the lungs postinfection In contrast, pneumoniae–infected Rag2−/−Il2rg−/− failed produce IL-22. These data suggested possible NK cells other innate...
Abstract Hematopoietic chimerism is known to promote donor-specific organ allograft tolerance; however, clinical translation has been impeded by the requirement for toxic immunosuppression and large doses of donor bone marrow (BM) cells. Here, we investigated in mice whether durable might be enhanced pre-treatment recipient with liposomal clodronate, a macrophage depleting agent, goal vacating BM niches preferential reoccupation hematopoietic stem cells (HSC). We found that clodronate...
NADPH oxidases (NOX's), and the reactive oxygen species (ROS) they produce, play an important role in host defense, thyroid hormone synthesis, apoptosis, gene regulation, angiogenesis other processes. However, overproduction of ROS by these enzymes is associated with cardiovascular disease, fibrosis, traumatic brain injury (TBI) diseases. Structural similarities between NOX's have complicated development specific inhibitors. Here, we report NCATS-SM7270, a small molecule optimized from...
Abstract Uterine dysfunctions lead to fertility disorders and pregnancy complications. Normal uterine functions at depend on crosstalk among multiple cell types in microenvironments. Here, we performed the spatial transcriptomics single-cell RNA-seq assays determine local gene expression profiles embryo implantation site of mouse uterus day 7.5 (D7.5). The transcriptomic annotation identified 11 domains distinct signatures, including a mesometrial myometrium, an anti-mesometrial decidua...
Abstract Non-myeloablative hematopoietic cell transplantation (HCT) is a curative option for individuals with sickle disease (SCD). Our traditional goal this approach has been to achieve state of mixed donor/recipient chimerism. Recently, we reported an increased risk hematologic malignancies (HMs) in adults SCD following graft failure or To evaluate the origin HMs, performed chimerism analyses 5 patients who developed HMs after non-myeloablative HCT. DNA was extracted from sorted peripheral...
Invariant natural killer T cells (NKTs) have intrinsic anti-tumor properties that make them promising candidates for chimeric antigen receptor (CAR)-based immunotherapies. Transgenic cytokine expression has been shown to enhance the potency of cellular immunotherapies, and we hypothesized co-expressing IL-18 alone or with IL-15 would boost CAR-NKT therapeutic potential. To test this hypothesis, generated retroviral constructs expressing and/or inducible caspase 9 (iC9) safety switch...
Expression of the chemokine receptor CXCR4 by many cancers correlates with aggressive clinical behavior. As part initial studies in a project whose goal was to quantify expression on non-invasively, we examined cancer samples immunohistochemistry using validated anti-CXCR4 antibody. Among solid tumors, found significant percentages major types kidney, lung, and pancreatic adenocarcinomas, and, notably, metastases clear cell renal carcinoma squamous lung. We particularly high adrenocortical...
Abstract Allogeneic hematopoietic cell transplantation (allo-HCT) offers a curative option for patients with certain non-malignant hematological diseases. High-dose post-transplant cyclophosphamide (PT-Cy) (200 mg/kg) and sirolimus (3 mg/kg), (HiC) synergistically induce stable mixed chimerism. Further, cytotoxic T lymphocyte-associated antigen-4 immunoglobulin (CTLA4-Ig), also known as Abatacept (Aba), promote immune tolerance allograft survival. Here, in major histocompatibility complex...
Abstract CD1d-reactive Va24-invariant NKT cells (NKTs) control tumor growth via yet poorly understood interactions with tumor-associated macrophages (TAMs) and other myeloid cells. TAMs comprise M1- M2-like subsets, but only CD163high are associated poor outcome in neuroblastoma (NB) tumors. Here, we demonstrate that NKTs selectively target contact-dependent independent mechanisms. Upon direct contact antigen-pulsed M1 or M2 macrophages, killed the latter. Although killing was strictly...
Abstract The breakthrough discoveries of checkpoint inhibitors in the field tumor immunology have driven clinical success immunotherapies for cancer, despite their beneficial efficacy only a small portion patients. This is due part to immuno-evasive mechanisms and inability immune system recognize antigens as foreign. As therapeutic approach effectively present these order elicit an anti-tumor response, we previously designed characterized allogenic, gp96-Ig secreting, cell-based vaccine...
Abstract The expression of the program death-1 (PD-1) receptor, a member CD28 costimulatory receptor family, is induced by T cell activation. It still controversial whether PD-1 plays an inhibitory role or act as stimulatory factor in yet to be determined if contributes age-related defect CTL response virus. We hypothesized that initiation stage, activation, and upregulated on activated cells delivers signal cells. In present study, we measured aged mice after adenovirus (Ad) infection...
Abstract Background: To explore Bushen Huoxue Decoction (BHD) mechanism in recurrent spontaneous abortion (RSA). Methods: We predicted and screened the action targets of four Traditional Chinese Medicine representatives by Systems Pharmacology Database Analysis Platform, searched disease RSA through Drugbank, DisGeNET, TTD databases. According to obtained drug targets, "drug target-disease target" interaction network was further analyzed constructed. The core target BHD for treating were...