Sri Lankans have relied on traditional medicine to meet their primary health needs since the beginning of time. This island is blessed with an enormous number medicinal plants, which play a very important role in medicine. However, lengthy documented history still composed are not scientifically proven mentioned abilities or activities. To obtain sound information from this medicine, extraction biologically active compounds these plants important. Also, maintain accuracy results obtained...

Plants are basically biosynthetic factories that contain various pathways produce phytochemicals with the help of different parts cell while specialized cells or tissues hoard and use them as needed. Human population has used this to their advantage for centuries. Aims: This review article aims provide a comprehensive guide qualitative phytochemical tests, focusing on principles, procedures, interpretation results. Scope Review: covers tests major groups, including alkaloids, flavonoids,...

Aims: Determination of antibacterial efficacy aqueous, methanol, dichloromethane, and hexane extracts from Jeffreycia zeylanica leaves against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa.
 Methodology: Completely ballooned J. were gathered, air-dried, milled into fine powder. Then macerated in all four selected solvents for about 1-2 weeks, the obtained by vacuum evaporation under reduced pressure. Antibacterial activity was performed aureus (ATCC25923), coli (ATCC...

Binding configuration of a de novo stapled peptide on SARS-CoV-2 spike protein, as predicted by molecular simulation. Stapled residues enhance stability while interacting engage key amino acids the protein receptor-binding domain.

The ClpB-DnaK bichaperone system reactivates aggregated cellular proteins and is essential for survival of bacteria, fungi, protozoa, plants under stress. AAA+ ATPase ClpB a promising target the development antimicrobials because loss its activity detrimental many pathogens no apparent orthologs are found in metazoans. We investigated presence several compounds that were previously described as inhibitor leads human p97, an antitumor target. discovered N

Mirabilis jalapa Linn. (Nyctaginaceae), commonly referred to as the Four O'clock plant (Sinhala name: Hendirikka), is a popular ornamental grown for beauty of its flowers and used in folklore remedies. This treat variety disease conditions around world. It widely abdominal colic, aphrodisiac, boils, diarrhea, inflammations, genitourinary disorders, muscular pain, other issues by people from various countries. contains several phytochemical compounds isolated parts, such alkaloids,...

Introduction: The term “cardiovascular diseases” (CVDs) refers to any disease affecting the heart or blood vessels. CVDs are most common cause of death worldwide and responsible reason for 10% deaths in early 20th century. These can emerge as myocardial infarction ischemic disease, stroke, congestive failure. For diagnosing CVDs, physical, radiological investigations, laboratory investigations cardiac enzymes lipid profile used. In clinical practices, cardiovascular risk prediction models...

Atherosclerotic coronary artery disease is a significant global health threat, impacting millions annually. Over time, plaque buildup narrows the arteries, reducing blood flow to heart muscle and resulting in myocardial ischemia. Timely diagnosis intervention are crucial for restoring preventing infarction. Given limited availability of screening diagnostic tests, early ischemia remains challenging. While cardiac troponin considered gold standard detecting injury, its effectiveness...

Aims: To evaluate antibacterial activity of aqueous, methanol, dichloromethane, and hexane extracts Artocarpus nobilis Thw. leaves stem bark against Escherichia coli Staphylococcus aureus using agar well diffusion method.
 Methodology: Matured fully expanded parts were collected, air-dried, grounded. The extraction was obtained a decoction method. Antibacterial performed (ATCC® 25923TM) 25922TM) method gentamicin used as positive control. whole experiment done in triplicates the...

Aims To investigate the in vitro anti-inflammatory activity of aqueous, methanol, dichloromethane (DCM), and hexane extracts Jeffreycia zeylanica leaves extracts. In protein (egg albumin) denaturation method human red blood cell (HRBC) membrane stabilization methods were used to evaluate leaf extracts.
 Methodology Plant collected, washed, air dried, obtained crude plant material. Cold maceration was extract materials. Aqueous, DCM, as solvents for maceration. Diclofenac sodium positive...

The genus Jeffreycia consists of about 109 species to have medicinal properties. zeylanica (Family: Asteraceae) is commonly referred as “Heen-botiya, Papula or Pupula in Sinhala and Kappilay Kuppilay Tamil”. It an endemic herbaceous plant Sri Lanka. Synonyms are Cacalia Kuntze, Eupatorium zeylanicum L., Gymnanthemum (L.), Vernonia (L.). This can create secondary metabolites that may substantial bioactivity. Extracts from leaves, stems aerial parts rich alkaloids, flavonoids, steroids,...

Clitoria ternatea commonly known as Butterfly pea is a standard Ayurvedic medicinal plant used in many parts of south Asian countries. Traditional plants are great alternative to find new treatments and for the development novel antimicrobials combat diseases. In Ayurveda traditional folk medicine several countries, decoction extracts made from C. recommended be various medical treatments. claimed possess antibacterial, antiviral, antifungal properties, which had been supported validated by...

Aims: To investigate in vitro anti-inflammatory activity of aqueous, methanol, dichloromethane, and hexane extracts Artocarpus nobilis Thw. leaves stem bark using heat-induced protein denaturation test (egg albumin denaturation).
 Methodology: About 500 g each matured, fully expanded were collected, washed air-dried. Leaves parts grounded to obtain a fine powder material. The extractions obtained the decoction extraction method. Anti-inflammatory was evaluated egg Diclofenac sodium used...

Objective: To assess the antihyaluronidase activity of methanolic leaf extract Indigofera tinctoria L (I. tinctoria) (family: Fabaceae/Leguminosae) and stem bark Stereospermum suaveolens DC (S. suaveolens) Bignoniaceae) in vitro with a view to develop an antiaging skin formulation. Materials Methods: The different concentrations (0.19 mg/mL, 0.38 0.75 1.5 3.0 mg/mL) I. tinctoria, S. suaveolens, reference drug epigallocatechin gallate (EGCG) (12.5 μg/mL, 25 50 100 200 μg/mL) were determined...

31Aug 2016 IN VITRO ANTIBACTERIAL ACTIVITY OF HEXANE, CHLOROFORM AND METHANOLIC EXTRACTS DIFFERENT PARTS ACRONYCHIA PEDUNCULATA GROWN SRI LANKA. R.D. Nimantha Karunathilaka , Athige Rajith Niloshan Silva Chathuranga Bharathee Ranaweera D.M.R.K. Dissanayake N.R.M. Nelumdeniya Ranjith Pathirana and W. D. Ratnasooriya. Department of Nursing Midwifery, Faculty Allied Health Sciences, General Sir John Kotelawala Defence University, Werahere, Sri Lanka Basic Werahera, Lanka. Medical Laboratory...

Ischemic heart disease (IHD) remains a leading cause of morbidity and mortality globally. In recent years research has increasingly focused on the interplay between white blood cells development progression IHD. Neutrophils, lymphocytes, Monocytes play an important role in immune system were found to have dual roles both protective mechanisms harmful effects. Neutrophils are traditionally viewed as first responders tissue injury. Their rapid infiltration aids clearance cellular debris...

Anaplasma phagocytophilum is an intracellular tick-transmitted bacterial pathogen that infects neutrophils in mammals and causes granulocytic anaplasmosis. In this study, we investigated the molecular chaperones ClpB DnaK from A. phagocytophilum. Escherichia coli, cooperates with its co-chaperones DnaJ GrpE ATP-dependent reactivation of aggregated proteins. Since not produced metazoans, it a promising target for developing antimicrobial therapies, which generates interest studies on...