A5004995588- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- Plant and Biological Electrophysiology Studies
- Mass Spectrometry Techniques and Applications
- Circadian rhythm and melatonin
- Molecular Communication and Nanonetworks
- Neural dynamics and brain function
Nagoya Institute of Technology
2020-2024
Abstract Channelrhodopsins (ChRs) are light-gated ion channels extensively applied as optogenetics tools for manipulating neuronal activity. All currently known ChRs comprise a large cytoplasmic domain, whose function is elusive. Here, we report the cation channel properties of KnChR, one photoreceptors from filamentous terrestrial alga Klebsormidium nitens , and demonstrate that domain KnChR modulates properties. constituted 7-transmembrane forming pore, followed by C-terminus moiety...
Abstract Channelrhodopsins (ChRs) are light-gated ion channels and invaluable tools for optogenetic applications. Recent developments in multicolor optogenetics, which different neurons controlled by multiple colors of light simultaneously, have increased the demand ChR mutants with more distant absorption wavelengths. Here we report 2.9 Å-resolution cryo-electron microscopy structure a from Klebsormidium nitens (KnChR), is one most blue-shifted ChRs. The elucidates 6- s-cis configuration...
Abstract Channelrhodopsins are a family of microbial rhodopsins that function as light-gated ion channel. We report the molecular properties novel channelrhodopsin KnRh3 from an evolutionary important filamentous terrestrial alga Klebsormidium nitens. is constituted 7-transmembrane domain, followed by long C-terminus moiety encodes peptidoglycan binding domain (FimV). When functionally expressed in mammalian cells, showed light-induced cation channel currents. The maximum action spectrum...