A5069215664- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- DNA Repair Mechanisms
- Mitochondrial Function and Pathology
- Antibiotic Resistance in Bacteria
- Analytical Chemistry and Sensors
- RNA Interference and Gene Delivery
- Electrochemical sensors and biosensors
- Advanced Chemical Sensor Technologies
University of Toronto
2014-2020
University at Buffalo, State University of New York
2012-2017
California State University, Fullerton
2015
North Dakota State University
2006
The faithful segregation, or "partition," of many low-copy number bacterial plasmids is driven by plasmid-encoded ATPases that are represented the P1 plasmid ParA protein. binds to nucleoid via an ATP-dependent nonspecific DNA (nsDNA)-binding activity, which essential for partition. also has a site-specific DNA-binding activity
The ATP-bound form of the Escherichia coli DnaA protein binds 'DnaA boxes' present in origin replication (oriC) and operator sites several genes, including dnaA, to co-ordinate their transcription with initiation replication. Hda protein, together β sliding clamp, stimulates ATPase activity via a process termed regulatory inactivation (RIDA), regulate DNA Here, we used mutant dnaN159 strain, which expresses β159 clamp gain insight into how actions are co-ordinated Elevated expression impeded...
Summary The ATP‐bound form of the Escherichia coli DnaA replication initiator protein remodels chromosomal origin replication, oriC , to load replicative helicase. primary mechanism for regulating activity involves Hda and β clamp proteins, which act together dramatically stimulate intrinsic DNA‐dependent ATPase via a process termed Regulatory Inactivation DnaA. In addition hyperinitiation, strains lacking hda function also exhibit cold sensitive growth at 30°C. Strains impaired other...