A5080001646- Bone health and treatments
- Mesenchymal stem cell research
- Bone Metabolism and Diseases
- Sarcoma Diagnosis and Treatment
- RNA Interference and Gene Delivery
- Bone Tissue Engineering Materials
- Cell death mechanisms and regulation
- Cancer Cells and Metastasis
- Cell Adhesion Molecules Research
- Multiple Myeloma Research and Treatments
- Cytokine Signaling Pathways and Interactions
- interferon and immune responses
- Orthopedic Infections and Treatments
- Virus-based gene therapy research
- 3D Printing in Biomedical Research
- Ubiquitin and proteasome pathways
- Bone fractures and treatments
- Orthopaedic implants and arthroplasty
- NF-κB Signaling Pathways
- Connective tissue disorders research
- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- Periodontal Regeneration and Treatments
- Cancer-related Molecular Pathways
- Radiopharmaceutical Chemistry and Applications
Nantes Université
2013-2025
Inserm
2014-2025
Centre National de la Recherche Scientifique
1996-2021
Cancéropôle Grand Ouest
2021
La Ligue Contre le Cancer
2013-2016
Centre de Chirurgie Plastique
2016
PhysioPathologie des Adaptations Nutritionnelles
2012
Institut National de Recherche en Santé Publique
2007-2011
The Enteric Nervous System in Gut and Brain Disorders
2011
IMT Atlantique
2007
Pancreatic tumors are the gastrointestinal cancer with worst prognosis in humans and a survival rate of 5% at 5 years. Nowadays, no chemotherapy has demonstrated efficacy terms for this cancer. Previous study focused on development new therapy by non thermal plasma showed significant effects tumor growth colorectal carcinoma glioblastoma. To allow targeted treatment, fibered (Plasma Gun) was developed its evaluation performed an orthotopic mouse model human pancreatic using MIA PaCa2-luc...
Abstract Targeting the ubiquitin-proteasome pathway has emerged as a potent anticancer strategy. Bortezomib, specific proteasome inhibitor, been approved for treatment of relapsed or refractory multiple myeloma. Multiple myeloma cell survival is highly dependent on Mcl-1 antiapoptotic molecules. In recent study, inhibitors induced accumulation that slowed down their proapoptotic effects. Consequently, we investigated role Bcl-2 family members in bortezomib-induced apoptosis. We found...
Abstract Osteosarcoma is the most frequent primary bone tumor that develops mainly in young, median age of diagnosis being 18 years. Despite improvement osteosarcoma treatment, survival rate only 30% at 5 years for patients with pulmonary metastases diagnosis. This warrants exploration new therapeutic options, and among them, osteoprotegerin (OPG), a naturally occurring protein inhibits resorption, very promising blocking vicious cycle between resorption proliferation takes place during...
Human mesenchymal stem cells (hMSC) have immunomodulative properties and, associated with calcium phosphate (CaP) ceramics, induce bone tissue repair. However, the mechanisms of osteoinduction by hMSC CaP are not clearly established, in particular role osteoclasts and macrophages. Biphasic (BCP) particles were implanted or without paratibial muscles nude mice. increased osteoblastic gene expression at 1 week, presence macrophages 2 4 weeks, osteoclastogenesis 8 osteogenesis weeks....
Deletion of the 17p13 chromosomal region [del(17p)] is associated with a poor outcome in multiple myeloma. Most studies have targeted TP53 gene for deletion analyses, although no study showed that this target. In order to address issue, we sequenced 92 patients myeloma at diagnosis, 54 del(17p) and 38 lacking del(17p). At least one mutation was found 20 patients, all them presenting The analysis location virtually occurred highly conserved domains involved DNA-protein interactions....
Titanium implants are widely used in orthopaedic and dental surgery.Surface properties play a major role cell tissue interactions.The adhesion differentiation of mesenchymal stem cells were studied as function nanostructures.Titanium surfaces with nanopores 30, 150 300 nm diameter prepared by physical vapour deposition.PCR arrays indicated that the expression integrins was modulated nanopore size.Human Mesenchymal Stem Cells (hMSCs) exhibited more branched morphology on Ti30 than other...
Interleukin (IL)-38 is a newly characterised cytokine that belongs to the IL-1 family. This expressed in rheumatoid arthritis (RA) synovial tissue and IL-38 deficient mice have exacerbated arthritis. Here, we analysed effect of overexpression joints arthritic mice, human macrophages fibroblasts vitro.Articular injections an adeno-associated virus (AAV) 2/8 encoding were performed collagen-induced (CIA), K/BxN serum transfer-induced (STIA) antigen-induced (AIA) mice. The was evaluated through...
Repair of large bone defects remains a significant clinical challenge. Bone marrow stromal cells (BMSCs), subset which is known as marrow-derived mesenchymal stem cells, show therapeutic potential for regeneration. However, their isolation, expansion and implantation will need to be conducted under good manufacturing practices (GMP) at separate locations. An investigation mimics this scenario where shall regenerated required before trials can initiated. Seven batches 100 million human...
Osteoclasts are bone-resorptive cells that differentiate from hematopoietic precursors upon receptor activator of nuclear factor κB ligand (RANKL) activation. Previous studies demonstrated IL-6 indirectly stimulates osteoclastogenesis through the production RANKL by osteoblasts. However, few data described direct effect on osteoclasts. To investigate this effect, we used several models: murine RAW264.7 cells, mouse bone marrow, and human blood monocytes. In three models used, addition...
Background Osteosarcoma is the most common malignant primary bone tumour in young adult treated by neo adjuvant chemotherapy, surgical tumor removal and multidrug chemotherapy. For correction of soft tissue defect consecutive to surgery and/or treatment, autologous fat graft has been proposed plastic reconstructive surgery. Principal Findings We report here a case late local recurrence osteosarcoma which occurred 13 years after initial pathology 18 months lipofilling procedure. Because such...
Abstract The possibility of using adipose tissue-derived stromal cells (ATSC) as alternatives to bone marrow-derived (BMSC) for repair has garnered interest due the accessibility, high cell yield, and rapid in vitro expansion ATSC. For clinical relevance, their forming potential comparison BMSC must be proven. Distinct differences between ATSC have been observed osteogenic vivo is not clear date. aim current study was compare osteogenesis human xenofree-expanded an ectopic nude mouse model...
We aimed to study the interactions between human mesenchymal stem cells and bone integration of nanostructured titanium implants.Nanopores 20, 30 50 nm were prepared by anodization at 5, 10 20 V in a mixture fluorhydric acetic acid. Ti nanostructures promoted early osteoblastic gene differentiation without osteogenic supplements. The osseointegration control implants was compared implantation rat tibias for 1 3 weeks.The significantly accelerated apposition bonding strength vivo correlation...
Mesenchymal stem cells (MSCs) and pericyte progenitors (PPs) are both perivascular with similar multipotential properties regardless of tissue origin. We compared the phenotype function 2 cell types derived from same bone-marrow samples but expanded in their respective media – conditions (endothelial growth medium [EGM-2]) for PPs standard (mesenchymal [MSM]) MSCs. After 3 weeks culture, whatever expansion medium, all showed characteristics (MSC markers adipo-osteo-chondroblastic...
Abstract Gastric cancer (GC) is the third cause of cancer-related mortality worldwide and often diagnosed at advanced stages disease. This makes development more comprehensive models efficient treatments crucial. One option based on repurposing already marketed drugs as adjuvants to chemotherapy. Accordingly, we have previously developed combination docetaxel cholesterol-lowering drug, lovastatin, a powerful trigger HGT-1 human GC cells’ apoptosis using 2D cultures. Because 3D models, known...
A major limitation of the 2D culture systems is that they fail to recapitulate in vivo 3D cellular microenvironment whereby cell-cell and cell-extracellular matrix (ECM) interactions occur. In this paper, a biomaterial scaffold mimics structure collagen fibers was produced by jet-spraying. This micro-fiber polycaprolactone (PCL) evaluated for human bone marrow mesenchymal stromal cells (MSCs) comparison with commercially available electrospun scaffold. The jet-sprayed scaffolds had larger...