A5042745265- Drug Transport and Resistance Mechanisms
- Plant Reproductive Biology
- Cholesterol and Lipid Metabolism
- RNA and protein synthesis mechanisms
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Plant-Microbe Interactions and Immunity
- Plant Parasitism and Resistance
- Digestive system and related health
- Lipid metabolism and biosynthesis
Institute of Bioorganic Chemistry, Polish Academy of Sciences
2020-2024
Adam Mickiewicz University in Poznań
2020-2023
The plant hormone auxin must be transported throughout plants in a cell-to-cell manner to affect its various physiological functions. ABCB transporters are critical for this polar distribution, but the regulatory mechanisms controlling their function is not fully understood. transport activity of ABCB1 was suggested regulated by physical interaction with FKBP42/Twisted Dwarf1 (TWD1), peptidylprolyl cis-trans isomerase (PPIase), all attempts demonstrate such PPIase TWD1 have failed so far. By...
ABCG46 of the legume Medicago truncatula is an ABC-type transporter responsible for highly selective translocation phenylpropanoids, 4-coumarate, and liquiritigenin, over plasma membrane. To investigate molecular determinants observed substrate selectivity, we applied a combination phylogenetic biochemical analyses, AlphaFold2 structure prediction, dynamics simulations, mutagenesis. We discovered unusually narrow transient access path to central cavity MtABCG46 that constitutes initial...
Abstract For G-type ATP-binding cassette (ABC) transporters, a hydrophobic “di-leucine motif” as part of extracellular gate has been described to separate large substrate-binding cavity from smaller upper and proposed act valve controlling drug extrusion. Here, we show that an L704F mutation in the Arabidopsis ABCG36/PDR8/PEN3 uncouples export auxin precursor indole-3-butyric acid (IBA) defense compound camalexin (CLX). Molecular dynamics simulations reveal increase free energy pulling...
Abstract Auxin transport activity of ABCB1 was suggested to be regulated by physical interaction with the FKBP42/Twisted Dwarf1 (TWD1), a bona fide peptidylprolyl cis-trans isomerase (PPIase), but all attempts demonstrate such PPIase on TWD1 have failed so far. By using structure-based approach we identified series surface-exposed proline residues in C-terminal nucleotide binding fold and linker Arabidopsis that do not alter protein stability or location its catalytic activity. P1.008...
Abstract Based on its proposed substrate preferences, the ABC transporter, ABCG36/PDR8/PEN3, from model plant Arabidopsis stands at cross-road between growth and defence. Recently, ABCG36 was shown to export a few indolic compounds, including auxin precursor, indole-3-butyric acid (IBA), be implicated in of major phytoalexin Arabidopsis, camalexin, although clear-cut proof camalexin transport activity is still lacking. Here we provide strong evidence that catalyses direct, ATP-dependent over...
ABCG46 of the legume Medicago truncatula is an ABC-type transporter responsible for highly selective translocation phenylpropanoids, 4-coumarate and liquiritigenin, over plasma membrane. To investigate molecular determinants observed substrate selectivity, we applied a combination phylogenetic biochemical analyses, AlphaFold2 structure prediction, dynamics simulations, mutagenesis. We discovered unusually narrow transient access path to central cavity MtABCG46 that constitutes initial filter...