Bacterial infections present an enormous problem causing human suffering and cost burdens to healthcare systems worldwide. Here we novel tunable antibacterial coatings which completely inhibit bacterial colonization by Staphylococcus epidermidis but allow normal adhesion spreading of osteoblastic cells. The are based on amine plasma polymer films loaded with silver nanoparticles. method preparation allows flexible control over the amount nanoparticles rate release ions.

This paper presents a novel and facile method for the generation of efficient antibacterial coatings which can be applied to practically any type substrate. Silver nanoparticles were stabilized with an adsorbed surface layer polyvinyl sulphonate (PVS). steric provided excellent colloidal stability, preventing aggregation over periods months. PVS-coated silver bound onto amine-containing surfaces, here produced by deposition allylamine plasma polymer thin film various substrates. SEM imaging...

A crude extract from the Australian desert plant Eremophila neglecta has recently been shown to possess antibacterial activity in a survey of candidate plants that may bear novel antimicrobial compounds. Bioassay-directed fractionation Et2O E. using broth microdilution assay led isolation three new serrulatane-type diterpenoids, 2,19-diacetoxy-8-hydroxyserrulat-14-ene (2), 8,19-dihydroxyserrulat-14-ene (3), and 8-hydroxyserrulat-14-en-19-oic acid (4), known o-naphthoquinone commonly referred...

To determine the antibacterial spectrum and cytotoxic activities of serrulatane compounds from Australian plant Eremophila neglecta.Antimicrobial 8,19-dihydroxyserrulat-14-ene (1) 8-hydroxyserrulat-14-en-19-oic acid (2) were tested against Gram-negative Gram-positive bacteria including human veterinary pathogens some multidrug-resistant isolates. Minimum inhibitory concentrations (MICs) minimum bactericidal (MBCs) determined by broth microdilution assay. Both exhibited activity all test...

Abstract Plant species of the genus Eremophila (Myoporaceae) are native to Australia and known produce a diverse range unusual secondary compounds. The purpose this research was examine antimicrobial activity 72 most which had not been subject any previous pharmacological testing. Organic extracts were screened for against Gram‐positive Gram‐negative bacteria yeasts medical importance. Extracts number showed selective with MICs active in 16 62 μg/ml Streptococcus species, 250 standard...

Ten new branched-chain fatty acid (BCFA) dimers with a substituted cyclohexene structure, five monomers, and two known (2E,4Z,6E)-5-(acetoxymethyl)tetradeca-2,4,6-trienoic its 5-hydroxymethyl analogue, were identified in the leaf extract of Eremophila oppositifolia subsp. angustifolia using combination HPLC-PDA-HRMS-SPE-NMR analysis semipreparative-scale HPLC. The could be classified as three types Diels-Alder reaction products formed between monomers at different sites unsaturation...

The purpose of this study was to assess the biofilm-removing efficacy and inflammatory activity a serrulatane diterpenoid, 8-hydroxyserrulat-14-en-19-oic acid (1), isolated from Australian medicinal plant Eremophila neglecta. Biofilm breakup compound 1 on established Staphylococcus epidermidis aureus biofilms compared antiseptic chlorhexidine antibiotic levofloxacin. In time-course study, deposited onto polypropylene mesh mimic wound dressing tested for biofilm removal. ex-vivo cytotoxicity...

SUMMARY Eremophila is the largest genus in plant tribe Myoporeae (Scrophulariaceae) and exhibits incredible morphological diversity across Australian continent. The Aboriginal Peoples recognize many species as important sources of traditional medicine, most frequently used parts being leaves. Recent phylogenetic studies have revealed complex evolutionary relationships between related genera tribe. Unique structurally diverse metabolites, particularly diterpenoids, are also a feature plants...

Australian Aboriginal people have a long history of relying on plants for the treatment various ailments and illnesses. Our ongoing collaborative research project initiated by Chuulangun Corporation (Cape York, Australia) has recently focussed revealing whether Kuuku I’yu plant medicines possess anticancer-related activities chemistry responsible this. Here, we present results from study Litsea glutinosa, used traditionally gastrointestinal disorders. Four known aporphine alkaloids...

Plants in the Australian genus Eremophila (Scrophulariaceae) have attracted considerable recent attention for their antimicrobial compounds, which possess a wide range of chemical structures. As they are typically associated with oily-waxy resin layer covering leaves and green branchlets, lucida is prominent among species containing pronounced sticky layer, this was considered interest assessing its antibacterial constituents. The n-hexane fraction crude acetone extract exhibited activity...

Extracts of Eremophila phyllopoda subsp. showed α-glucosidase and PTP1B inhibitory activity with IC50 values 19.6 13.6 μg/mL, respectively. High-resolution α-glucosidase/PTP1B/radical scavenging profiling was performed to establish a triple high-resolution inhibition profile that allowed direct pinpointing the constituents responsible for one or more observed bioactivities. Subsequent targeted isolation purification by analytical-scale HPLC led identification 21 previously undescribed...

The Australian plant Acacia ligulata has a number of traditional food and medicinal uses by Aboriginal people, although no bioactive compounds have previously been isolated from this species. Bioassay-guided fractionation an ethanolic extract the mature pods A. led to isolation two new echinocystic acid triterpenoid saponins, ligulatasides A (1) B (2), which differ in fine structure their glycan substituents. Their structures were elucidated on basis 1D 2D NMR, GC-MS, LC-MS/MS, saccharide...

Eremophila (Scrophulariaceae) is a genus of Australian desert plants, which have been used by Aboriginal people for various medicinal purposes. Crude extracts the leaf resin glabra (R.Br.) Ostenf. showed α-glucosidase and protein tyrosine phosphatase 1B (PTP1B) inhibitory activity with IC50 values 19.3 ± 1.2 μg/mL 11.8 2.1 μg/mL, respectively. Dual α-glucosidase/PTP1B high-resolution inhibition profiling combined HPLC-PDA-HRMS NMR were to isolate identify compounds providing these...

In this study, an extract of the leaves Eremophila clarkei Oldfield & F.Muell. showed protein tyrosine phosphatase 1B (PTP1B) inhibitory activity with IC50 value 33.0 μg/mL. The was therefore investigated by high-resolution PTP1B inhibition profiling to pinpoint constituents responsible for activity. Subsequent isolation and purification using analytical-scale HPLC led identification eight previously undescribed decipiene diterpenoids, eremoclarkanes A-H, as well eremoclarkic acid, a...

Crude extract of Eremophila spathulata leaves was investigated by semi-preparative scale high-performance liquid chromatography (HPLC), analytical HPLC, and hyphenated chromatography-photodiode array-high-resolution mass spectrometry-nuclear magnetic resonance (HPLC-PDA-HRMS-SPE-NMR), which afforded seven previously unreported caryophyllane sesquiterpenoids. Semi-preparative separation the crude (1R*,4R*,9S*,E)-8-formyl-11,11-dimethylbicyclo[7.2.0]undec-7-ene-4-carboxylic acid (5)...

Hydrophilically substituted diterpenes of the structural class serrulatanes have attracted attention as novel antibacterial compounds that are effective even against multidrug-resistant Staphylococcus aureus, a key bacterium involved in human infections. The mechanism action has, however, not been established yet. Available data on structure–activity relationships suggest aromatic hydroxy group is essential for activity, and strongest activity has found naphthyl compounds. In this context,...

Acacia ligulata A.Cunn. ex Benth. (Fabaceae: Mimosoideae) is a native Australian plant used traditionally by Aboriginal groups. This study was undertaken to investigate the bioactivity of A. extracts and evaluate their chemical composition. Potential antibacterial, cytotoxic enzyme inhibitory effects relevant traditional medicinal food uses species were examined LC-MS/MS performed Antibacterial activity observed for bark leaf with an MIC extract 62.5 μg/mL against Streptococcus pyogenes. Pod...

Previously undescribed eremane, viscidane, and isozizaene diterpenoids, eremorigidanes A-F, along with six known O-methylated flavonoids three triterpenoids were isolated identified from the leaves of Eremophila rigida Chinnock by combined use high-resolution PTP1B inhibition profiling, semipreparative- analytical-scale HPLC separations, HPLC-PDA-HRMS analysis, NMR spectroscopy. The absolute configuration unreported diterpenoids determined comparison their experimental calculated ECD spectra...

Abstract Eremophila is the largest genus in plant tribe Myoporeae (Scrophulariaceae) and exhibits incredible morphological diversity across Australian continent. The Aboriginal Peoples recognize many species as important sources of traditional medicine, most frequently used parts being leaves. Recent phylogenetic studies have revealed complex evolutionary relationships between related genera tribe. Unique structurally diverse metabolites, particularly diterpenoids, are also a feature plants...