A5101902043- Mitochondrial Function and Pathology
- Cancer-related Molecular Pathways
- DNA Repair Mechanisms
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Microtubule and mitosis dynamics
- Peroxisome Proliferator-Activated Receptors
- Neurological Disease Mechanisms and Treatments
- RNA Research and Splicing
- DNA and Biological Computing
- Mesenchymal stem cell research
- Steroid Chemistry and Biochemistry
- Protease and Inhibitor Mechanisms
- Cancer-related molecular mechanisms research
- Macrophage Migration Inhibitory Factor
- Advanced biosensing and bioanalysis techniques
- Cell Adhesion Molecules Research
- Autophagy in Disease and Therapy
- Cellular Automata and Applications
- Glycosylation and Glycoproteins Research
- Medicinal Plants and Bioactive Compounds
- Immune cells in cancer
- Cancer, Hypoxia, and Metabolism
- Enzyme Production and Characterization
- Enzyme Structure and Function
Beijing Institute of Genomics
2015-2024
Chinese Academy of Sciences
2015-2024
Shanghai Center For Bioinformation Technology
2024
Guangxi Academy of Agricultural Science
2023
Biotechnology Research Institute
2023
University of Chinese Academy of Sciences
2017
Hubei University of Technology
2015
Hebei North University
2015
PRKN/parkin activation through phosphorylation of its ubiquitin and ubiquitin-like domain by PINK1 is critical in mitophagy induction for eliminating the damaged mitochondria. Deubiquitinating enzymes (DUBs) functionally reversing PRKN ubiquitination are controlling magnitude PRKN-mediated process. However, potential DUBs that directly target antagonize pro-mitophagy effect remains to be identified characterized. Here, we demonstrated USP33/VDU1 localized at outer membrane mitochondria...
Xeroderma pigmentosum group D (XPD/ERCC2) encodes an ATP-dependent helicase that plays essential roles in both transcription and nucleotide excision repair of nuclear DNA, however, whether or not XPD exerts similar functions mitochondria remains elusive. In this study, we provide the first evidence is localized inner membrane mitochondria, cells under oxidative stress showed enhanced recruitment into mitochondrial compartment. Furthermore, reactive oxygen species production levels...
Human RecQL4 helicase plays critical roles in the maintenance of genomic stability. Mutations results three clinically related autosomal recessive disorders: Rothmund-Thomson syndrome (RTS), RAPADILINO, and Baller-Gerold syndrome. In addition to several premature aging features, RTS patients are characterized by aneuploidy involving either loss or gain a single chromosome. Chromosome mosaicism isochromosomes chromosomes 2, 7, 8 have been reported RecQL4-deficient patients, but precise role...
Abstract Ubiquitination-dependent DNA damage response (DDR) signals play a critical role in the cellular choice of repair pathways. Human helicase RecQL4 participates replication and repair, loss is associated with autosomal recessive genetic disorders characterized by genomic instability features. In an earlier study, was isolated as stable complex that contained two ubiquitin ligases N-end rule (UBR1 UBR2). However, it unknown whether or not ubiquitination status for its function. Here, we...
Abstract DNA storage is a new digital data technology based on specific encoding and decoding methods between 0 1 binary codes of A-T-C-G quaternary DNAs, which expected to develop into major form in the future due its advantages (such as high density, long time, low energy consumption, convenience for carrying, concealed transportation multiple encryptions). In this review, we mainly summarize recent research advances four main technology: direct mapping method early-stage, fountain code...
RNA binding protein RBM10 participates in various metabolism, and its decreased expression or loss of function by mutation has been identified many human cancers. However, how dysregulation contributes to cancer pathogenesis remains be determined. Here, we found that was breast tumors, patients with low presented poorer survival rates. depletion cells significantly promotes the cellular proliferation migration. We further demonstrated forms a triple complex YBX1 phosphatase 1B (PPM1B), which...
Vascular endothelial injury caused by post-hemorrhagic shock mesenteric lymph (PHSML) return is an important manifestation during refractory hemorrhagic shock. By using human umbilical vein cells (HUVECs) and transcriptome analysis, the present study sought to investigate molecular mechanism underlying adverse effect of PHSML on vascular endothelium. was collected from male rats underwent following resuscitation, while normal (NML) harvested sham rats. HUVECs were incubated with culture...
MMS19 localizes to the cytoplasmic and nuclear compartments involved in transcription nucleotide excision repair (NER). However, whether mitochondria, where it plays a role maintaining mitochondrial genome stability, remains unknown. In this study, we provide first evidence that is localized inner membrane of mitochondria participates mtDNA oxidative damage repair. knockdown led dysfunctions including decreased copy number, diminished capacity, elevated levels common deletion after stress....
ABSTRACT The de‐ubiquitinase USP33 has been shown to possess either tumour‐promoting or inhibitory effect on human cancer cells. However, all these findings are mainly based in vitro cell culture models, and the vivo evidence, which is more plausible digest functional role of carcinogenic process, still lacking. Here, we demonstrate that modulates DNA damage responses including cycle arrest apoptosis induction through associating with p53. It directly interacts p53 mediate its...
Since the first case of kidney transplant succeeded in 1954, technology is relatively mature renal transplant. After operation one year and five years survival rate can reach more than 90% 80%, respectively. But it still has to face rejection. The traditional way apply immune inhibitors prevent side effects brought by immunosuppressant seriously affects patients’ quality life. So induction tolerance become a research hotspot. Vascularized bone marrow transplantation (VBMT) developed on...
As a city's landmark building, railway passenger stations are often large in space, crowded and extremely comfortable, which resulted high energy consumption.Taking Taiyuan south station as case, this paper introduced the green energy-saving design of adaptable climate through natural ventilation, palisade structure, shading lighting, ventilation heating,and so on.Meanwhile, effects were verified by simulation experimental test.
DSPAα1 is a potent rude thrombolytic protein with high medicative value. has two natural N-glycan sites (N153Q-S154-S155, N398Q-K399-T400) that may lead to immune responses when administered in vivo. We aimed study the effect of its N-glycosylation on vitro and vivo by mutating these sites. In this experiment, four single mutants one double mutant were predicted expressed Pichia pastoris. When N398Q-K399-T400 site was mutated, fibrinolytic activity reduced 75%. N153Q-S154-S155 inactivated as...
前期研究表明，MgCl2胁迫不但可以提高TGase的产量，还会影响微生物次级代谢产物合成的一个重要调节因子LuxR家族蛋白的表达.为了研究LuxR家族蛋白对茂原链霉菌(Streptomyces mobaraensis) TGase生物合成以及菌体生长的调控作用，利用pKC1139为载体，构建luxR基因阻断质粒，通过PEG介导的质粒转化将其转入S. mobaraensis原生质体中，利用单交换同源重组法将整个重组质粒插入到茂原链霉菌基因组中构建S. mobaraensis luxR家族蛋白基因阻断突变菌.利用菌体干重法对比突变菌和野生菌菌体生长变化，利用比色法研究两株菌TGase生物合成的变化，找到LuxR家族蛋白基因表达与TGase生物合成之间的关系.结果发现，本研究采用单交换同源重组的方法可以成功地构建出S. luxR基因阻断突变菌株.通过对比野生菌与阻断菌株生长和TGase活力的变化发现阻断luxR基因会导致菌体生长受阻，TGase合成延迟，并且表达量显著下降.这些研究结果表明本文研究的luxR基因可能是S. mobaraensis合成TGase必不可少的正向调控因子.